LOCUS       sequence-74576-e         970 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 970)
  AUTHORS   Schaffer U, Schlosser A, Muller KM, Schafer A, Katava N, Baumeister 
            R, Schulze E
  TITLE     SnAvi--a new tandem tag for high-affinity protein-complex 
            purification.
  JOURNAL   Nucleic Acids Res. 2010 Apr;38(6):e91. doi: 10.1093/nar/gkp1178. 
            Epub 2010 Jan 4.
  PUBMED    20047968
REFERENCE   2  (bases 1 to 970)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2010-04"; volume: "38(6):e91. doi"; pages: " 
            10.1093/nar/gkp1178. Epub 2010 Jan 4"
COMMENT     Sequence Label: pBY2807
FEATURES             Location/Qualifiers
     source          1..970
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(108..232)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             327..347
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             354..374
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             join(393..395,396..398,399..911)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIE"
     primer_bind     complement(438..459)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(699..718)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 ggctgatctc agccaccgcg aaaatgacat caaaaacgcc attaacctga tgttctgggg
       61 aatataaatg tcaggctccc ttatacacag ccagtctgca ggtcgaccat agtgactgga
      121 tatgttgtgt tttacagtat tatgtagtct gttttttatg caaaatctaa tttaatatat
      181 tgatatttat atcattttac gtttctcgtt cagctttctt gtacaaagtg gtcgtcccac
      241 cggttactac catggctcca cgaccatcca acaaacgtct ccagcagtcc ggtatgccac
      301 gaccctccaa caagcgtctt caacaggaga acctttactt tcaaggtcaa ttggaaaatc
      361 tctatttcca gggaccacca gcgccaccac agatggtgag caagggcgag gagctgttca
      421 ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg
      481 tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca
      541 ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc
      601 agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc
      661 ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc
      721 gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg
      781 acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca
      841 acgtctatat catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc
      901 acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg
      961 gcgacggccc
//
