LOCUS       sequence-74586-e         847 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid for expression of chiRNA under the control of the Drosophila
            snRNA:U6:96Ab promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 847)
  AUTHORS   Gratz SJ, Cummings AM, Nguyen JN, Hamm DC, Donohue LK, Harrison MM, 
            Wildonger J, O'Connor-Giles KM
  TITLE     Genome engineering of Drosophila with the CRISPR RNA-guided Cas9 
            nuclease.
  JOURNAL   Genetics. 2013 May 24.
  PUBMED    23709638
REFERENCE   2  (bases 1 to 847)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2013 May 24."
COMMENT     Sequence Label: pU6-BbsI-chiRNA
FEATURES             Location/Qualifiers
     source          1..847
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     53..69
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     53..69
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     misc_RNA        complement(98..173)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(192..591)
                     /label=dU6-2 promoter
                     /note="RNA polymerase III promoter for Drosophila U6-2 
                     snRNA (Das et al., 1987)"
     primer_bind     complement(603..619)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(604..620)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(644..663)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(645..663)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(670..687)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(670..686)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(679..701)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 taaccctcac taaagggaac aaaagctgga gctccaccgc ggtggcggcc gctctagaac
       61 tagtggatcc cccgggctgc aggaattcga taaaaaagca ccgactcggt gccacttttt
      121 caagttgata acggactagc cttattttaa cttgctattt ctagctctaa aacaggtctt
      181 ctcgaagacc cgaagtattg aggaaaacat acctatataa atgatcaaca tcaggaaaga
      241 gcagttgaga attataagaa ttggcaaatg gtcttaagaa ccctctgctt aagattttca
      301 aaatttcctt tagaatcaaa gtgtcctatt gttcgtttgt tgaaaacaca gttcgattta
      361 ttgactataa taaattgata gttttaaata tagaggcacg actaagagag tttggttttg
      421 ttttggttag ggaccaaaaa aagtatacat aacgaataaa aaaggattta agtgcaaatg
      481 gtaaaaaagt gtcgagtttt tcttgagttg attgtgctgt aatgagactc tgcattcggc
      541 atttgactcg gcttttccta ctcgtgccgt atttcaggct gcaagtcgaa catcaagctt
      601 atcgataccg tcgacctcga gggggggccc ggtacccaat tcgccctata gtgagtcgta
      661 ttacaattca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca
      721 acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg
      781 caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatgggacg cgccctgtag
      841 cggcgca
//
