LOCUS       sequence-74590-e         972 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Donor plasmid for homologous recombination that expresses dsRED 
            constitutively using the Aedes aegypti polyubiquitin (PUb) 
            promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 972)
  TITLE     Aedes homologous recombination donor plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 972)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: PSL1180polyUBdsRED
FEATURES             Location/Qualifiers
     source          1..972
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    82..203
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(93..112)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     147..166
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(386..409)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(584..603)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     801..821
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
ORIGIN
        1 ggtgcacggc ccacgtggcc actagtactt ctcgagctct gtacatgtcc gcggtcgcga
       61 cgtacgcgta tcgataagct ttaagataca ttgatgagtt tggacaaacc acaactagaa
      121 tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca
      181 ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc
      241 agggggaggt gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt ggtatggctg
      301 attatgatct agagtcgcgg ccgctacagg aacaggtggt ggcggccctc ggtgcgctcg
      361 tactgctcca cgatggtgta gtcctcgttg tgggaggtga tgtccagctt ggagtccacg
      421 tagtagtagc cgggcagctg cacgggcttc ttggccatgt agatggactt gaactccacc
      481 aggtagtggc cgccgtcctt cagcttcagg gccttgtgga tctcgccctt cagcacgccg
      541 tcgcgggggt acaggcgctc ggtggaggcc tcccagccca tggtcttctt ctgcattacg
      601 gggccgtcgg aggggaagtt cacgccgatg aacttcacct tgtagatgaa gcagccgtcc
      661 tgcagggagg agtcctgggt cacggtcacc acgccgccgt cctcgaagtt catcacgcgc
      721 tcccacttga agccctcggg gaaggacagc ttcttgtagt cggggatgtc ggcggggtgc
      781 ttcacgtaca ccttggagcc gtactggaac tggggggaca ggatgtccca ggcgaagggc
      841 agggggccgc ccttggtcac cttcagcttc acggtgttgt ggccctcgta ggggcggccc
      901 tcgccctcgc cctcgatctc gaactcgtgg ccgttcacgg tgccctccat gcgcaccttg
      961 aagcgcatga ac
//
