LOCUS       sequence-74592-e         931 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of a catalytically inactive, human codon-optimized 
            Cas9-BFP fusion under the control of Murine Stem Cell retroVirus LTR
            promoter for mammalian gene knockdown.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 931)
  AUTHORS   Qi LS, Larson MH, Gilbert LA, Doudna JA, Weissman JS, Arkin AP, Lim 
            WA
  TITLE     Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific 
            Control of Gene Expression.
  JOURNAL   Cell. 2013 Feb 28;152(5):1173-83. doi: 10.1016/j.cell.2013.02.022.
  PUBMED    23452860
REFERENCE   2  (bases 1 to 931)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2013.02"; journalName: "Cell"; date: "2013-02-28- 
            28"; volume: "152"; issue: "5"; pages: "1173-83"
COMMENT     Sequence Label: pdCas9::BFP-humanized
FEATURES             Location/Qualifiers
     source          1..931
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 cctcgcggat aagtttgtcg ttttcgtcgt acttggtatt catgcgtgag tcaaggattt
       61 gggcgacatg tttagtaatc tgccgagtct ccacaagttg ccgtttaatg aatcctgctt
      121 tgtccagttc gctcagtccg cccctctcag ccttagtgag attgtcgaac ttccgctgag
      181 tgatcagctt tgcgttcaga agctggcgcc aatagttctt catctttttc acgacttcct
      241 ctgagggcac gttatctgac tttccccggt tcttgtctga gcgagtcagc accttattgt
      301 caattgagtc gtctttgagg aagctctgag ggacgatggc gtccacgtcg tagtctgaga
      361 gcctgttgat atcaagctct tggtccacat acatatcgcg tccattttga aggtagtaca
      421 gatagagctt ctcattctga agctgggtgt tttccaccgg gtgctcttta aggatctgtg
      481 acccgagctc cttaatccct tcctcaattc ttttcatccg ctccctgctg ttcttctgtc
      541 ccttctgggt agtctggttt tccctggcca tctcgatgac aatgttctct ggtttatggc
      601 gacccatcac cttgaccagc tcgtccacga ctttcacggt ctggagaata cccttcttaa
      661 tagcgggtga accagccaga tttgcgatat gctcgtgcag tgagtcacct tgtccagaca
      721 cttgggcctt ctggatgtcc tccttaaagg tcagggaatc atcatggata agttgcatga
      781 agttgcgatt agcgaatccg tcgctcttaa ggaaatcaag aatggtcttt ccgctctgtt
      841 tgtcccggat tccgttgatg agtttgcggc taaggcgtcc ccatccggta tatcttctcc
      901 gtttaagttg cttcatcact ttatcgtcga a
//
