LOCUS       sequence-74599-e         872 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of human Kdm2b CxxC-PHD domain with N-His6-TEV 
            and C-Avi tags. For use in BioCAP non-methylated DNA purification. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 872)
  TITLE     Klose Lab BioCAP plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 872)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pNIC28-hKdm2b-CxxC-PHD
FEATURES             Location/Qualifiers
     source          1..872
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             4..26
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             37..54
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             79..99
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             562..606
                     /codon_start=1
                     /product="peptide tag that allows for enzymatic 
                     biotinylation"
                     /label=AviTag(TM)
                     /translation="GLNDIFEAQKIEWHE"
     CDS             672..689
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(742..760)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      756..803
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 aattttgttt aactttaaga aggagatata catatgcacc atcatcatca tcattcttct
       61 ggtgtagatc tgggtaccga gaacctgtac ttccaatcca tgaaccggac aacggcagga
      121 gctcggcggc gccggacgcg atgccgcaag tgcgaggcct gcctgcggac cgagtgcgga
      181 gagtgccact tctgcaagga catgaagaag ttcgggggcc ccgggcgcat gaagcagagc
      241 tgcatcatgc ggcagtgcat cgcgccagtg ctgccccaca ccgccgtgtg ccttgtgtgt
      301 ggcgaggcgg ggaaggaaga cacggtggaa gaggaggaag gcaagtttaa cctcatgctc
      361 atggagtgct ccatctgcaa tgaaatcatc caccctggat gccttaagat taaggagtca
      421 gagggtgtgg tcaacgacga gcttccaaac tgctgggagt gtccgaagtg taaccacgcc
      481 ggcaagaccg ggaaacaaaa gcgtggccct ggctttaagt acgcctccaa cctgcccggc
      541 tccctgctca aggagggtgg cggtctgaac gacatcttcg aggctcagaa aatcgaatgg
      601 cacgaataac agtaaaggtg gatacggatc cgaattcgag ctccgtcgac aagcttgcgg
      661 ccgcactcga gcaccaccac caccaccact gagatccggc tgctaacaaa gcccgaaagg
      721 aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta
      781 aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggattg gcgaatggga
      841 cgcgccctgt agcggcgcat taagcgcggc gg
//
