LOCUS       sequence-74604-e         909 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  cDNA of Akt1 with myristyolation signal and point mutation from K to
            M at position 179 in cloning vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 909)
  AUTHORS   Aoki M, Batista O, Bellacosa A, Tsichlis P, Vogt PK
  TITLE     The akt kinase: molecular determinants of oncogenicity.
  JOURNAL   Proc Natl Acad Sci U S A. 1998 Dec 8;95(25):14950-5.
  PUBMED    9843996
REFERENCE   2  (bases 1 to 909)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "1998-12-8"; volume: "95(25)"; pages: 
            "14950-5"
COMMENT     Sequence Label: pBSFI-myr-akt-K179M
FEATURES             Location/Qualifiers
     source          1..909
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             79..99
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="MGSSKSK"
ORIGIN
        1 agggaacaaa agctggagct ggccattacg gccgcggccg cctctagagg atctggtacc
       61 acgcgtatcg ataagcttat ggggagcagc aagagcaagc ccaagtctag aatgaacgac
      121 gtagccattg tgaaggaggg ctggctgcac aaacgagggg aatatattaa aacctggcgg
      181 ccacgctact tcctcctcaa gaacgatggc acctttattg gctacaagga acggcctcag
      241 gatgtggatc agcgagagtc cccactcaac aacttctcag tggcacaatg ccagctgatg
      301 aagacagagc ggccaaggcc caacaccttt atcatccgct gcctgcagtg gaccacagtc
      361 attgagcgca ccttccatgt ggaaacgcct gaggagcggg aagaatgggc caccgccatt
      421 cagactgtgg ccgatggact caagaggcag gaagaagaga cgatggactt ccgatcaggc
      481 tcacccagtg acaactcagg ggctgaagag atggaggtgt ccctggccaa gcccaagcac
      541 cgtgtgacca tgaacgagtt tgagtacctg aaactactgg gcaagggcac ctttgggaaa
      601 gtgattctgg tgaaagagaa ggccacaggc cgctactatg ccatgatgat cctcaagaag
      661 gaggtcatcg tcgccaagga tgaggttgcc cacacgctta ctgagaaccg tgtcctgcag
      721 aactctaggc atcccttcct tacggccctc aagtactcat tccagaccca cgaccgcctc
      781 tgctttgtca tggagtatgc caacgggggc gagctcttct tccacctgtc tcgagagcgc
      841 gtgttctccg aggaccgggc ccgcttctat ggtgcggaga ttgtgtctgc cctggactac
      901 ttgcactcc
//
