LOCUS       sequence-74613-e         874 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  cDNA of Akt1 with myristyolation signal and point mutation from T to
            A at position 308 in cloning vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 874)
  AUTHORS   Aoki M, Batista O, Bellacosa A, Tsichlis P, Vogt PK
  TITLE     The akt kinase: molecular determinants of oncogenicity.
  JOURNAL   Proc Natl Acad Sci U S A. 1998 Dec 8;95(25):14950-5.
  PUBMED    9843996
REFERENCE   2  (bases 1 to 874)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "1998-12-8"; volume: "95(25)"; pages: 
            "14950-5"
COMMENT     Sequence Label: pBSFI-myr-akt-T308A
FEATURES             Location/Qualifiers
     source          1..874
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             77..97
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="MGSSKSK"
ORIGIN
        1 ggaacaaaag ctggagctgg ccattacggc cgcggccgcc tctagaggat ctggtaccac
       61 gcgtatcgat aagcttatgg ggagcagcaa gagcaagccc aagtctagaa tgaacgacgt
      121 agccattgtg aaggagggct ggctgcacaa acgaggggaa tatattaaaa cctggcggcc
      181 acgctacttc ctcctcaaga acgatggcac ctttattggc tacaaggaac ggcctcagga
      241 tgtggatcag cgagagtccc cactcaacaa cttctcagtg gcacaatgcc agctgatgaa
      301 gacagagcgg ccaaggccca acacctttat catccgctgc ctgcagtgga ccacagtcat
      361 tgagcgcacc ttccatgtgg aaacgcctga ggagcgggaa gaatgggcca ccgccattca
      421 gactgtggcc gatggactca agaggcagga agaagagacg atggacttcc gatcaggctc
      481 acccagtgac aactcagggg ctgaagagat ggaggtgtcc ctggccaagc ccaagcaccg
      541 tgtgaccatg aacgagtttg agtacctgaa actactgggc aagggcacct ttgggaaagt
      601 gattctggtg aaagagaagg ccacaggccg ctactatgcc atgaagatcc tcaagaagga
      661 ggtcatcgtc gccaaggatg aggttgccca cacgcttact gagaaccgtg tcctgcagaa
      721 ctctaggcat cccttcctta cggccctcaa gtactcattc cagacccacg accgcctctg
      781 ctttgtcatg gagtatgcca acgggggcga gctcttcttc cacctgtctc gagagcgcgt
      841 gttctccgag gaccgggccc gcttctatgg tgcg
//
