LOCUS       sequence-74615-e         896 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  cDNA of Akt1 with myristyolation signal and point mutation from S to
            A at position 473 in cloning vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 896)
  AUTHORS   Aoki M, Batista O, Bellacosa A, Tsichlis P, Vogt PK
  TITLE     The akt kinase: molecular determinants of oncogenicity.
  JOURNAL   Proc Natl Acad Sci U S A. 1998 Dec 8;95(25):14950-5.
  PUBMED    9843996
REFERENCE   2  (bases 1 to 896)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "1998-12-8"; volume: "95(25)"; pages: 
            "14950-5"
COMMENT     Sequence Label: pBSFI-myr-akt-S473A
FEATURES             Location/Qualifiers
     source          1..896
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             81..101
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="MGSSKSK"
ORIGIN
        1 aaagggaaca aaagctggag ctggccatta cggccgcggc cgcctctaga ggatctggta
       61 ccacgcgtat cgataagctt atggggagca gcaagagcaa gcccaagtct agaatgaacg
      121 acgtagccat tgtgaaggag ggctggctgc acaaacgagg ggaatatatt aaaacctggc
      181 ggccacgcta cttcctcctc aagaacgatg gcacctttat tggctacaag gaacggcctc
      241 aggatgtgga tcagcgagag tccccactca acaacttctc agtggcacaa tgccagctga
      301 tgaagacaga gcggccaagg cccaacacct ttatcatccg ctgcctgcag tggaccacag
      361 tcattgagcg caccttccat gtggaaacgc ctgaggagcg ggaagaatgg gccaccgcca
      421 ttcagactgt ggccgatgga ctcaagaggc aggaagaaga gacgatggac ttccgatcag
      481 gctcacccag tgacaactca ggggctgaag agatggaggt gtccctggcc aagcccaagc
      541 accgtgtgac catgaacgag tttgagtacc tgaaactact gggcaagggc acctttggga
      601 aagtgattct ggtgaaagag aaggccacag gccgctacta tgccatgaag atcctcaaga
      661 aggaggtcat cgtcgccaag gatgaggttg cccacacgct tactgagaac cgtgtcctgc
      721 agaactctag gcatcccttc cttacggccc tcaagtactc attccagacc cacgaccgcc
      781 tctgctttgt catggagtat gccaacgggg gcgagctctt cttccacctg tctcgagagc
      841 gcgtgttctc cgaggaccgg gcccgcttct atggtgcgga gattgtgtct gccctg
//
