LOCUS       sequence-74620-e         899 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  cDNA of Akt1 with myristyolation signal and point mutation from T to
            A at position 308 and S to A at position 473 in cloning vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 899)
  AUTHORS   Aoki M, Batista O, Bellacosa A, Tsichlis P, Vogt PK
  TITLE     The akt kinase: molecular determinants of oncogenicity.
  JOURNAL   Proc Natl Acad Sci U S A. 1998 Dec 8;95(25):14950-5.
  PUBMED    9843996
REFERENCE   2  (bases 1 to 899)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "1998-12-8"; volume: "95(25)"; pages: 
            "14950-5"
COMMENT     Sequence Label: pBSFI-myr-akt-T308A/S473A
FEATURES             Location/Qualifiers
     source          1..899
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             78..98
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="MGSSKSK"
ORIGIN
        1 gggaacaaaa gctggagctg gccattacgg ccgcggccgc ctctagagga tctggtacca
       61 cgcgtatcga taagcttatg gggagcagca agagcaagcc caagtctaga atgaacgacg
      121 tagccattgt gaaggagggc tggctgcaca aacgagggga atatattaaa acctggcggc
      181 cacgctactt cctcctcaag aacgatggca cctttattgg ctacaaggaa cggcctcagg
      241 atgtggatca gcgagagtcc ccactcaaca acttctcagt ggcacaatgc cagctgatga
      301 agacagagcg gccaaggccc aacaccttta tcatccgctg cctgcagtgg accacagtca
      361 ttgagcgcac cttccatgtg gaaacgcctg aggagcggga agaatgggcc accgccattc
      421 agactgtggc cgatggactc aagaggcagg aagaagagac gatggacttc cgatcaggct
      481 cacccagtga caactcaggg gctgaagaga tggaggtgtc cctggccaag cccaagcacc
      541 gtgtgaccat gaacgagttt gagtacctga aactactggg caagggcacc tttgggaaag
      601 tgattctggt gaaagagaag gccacaggcc gctactatgc catgaagatc ctcaagaagg
      661 aggtcatcgt cgccaaggat gaggttgccc acacgcttac tgagaaccgt gtcctgcaga
      721 actctaggca tcccttcctt acggccctca agtactcatt ccagacccac gaccgcctct
      781 gctttgtcat ggagtatgcc aacgggggcg agctcttctt ccacctgtct cgagagcgcg
      841 tgttctccga ggaccgggcc cgcttctatg gtgcggagat tgtgtctgcc ctggactac
//
