LOCUS       sequence-74623-e       12630 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes pgk, gapA, aceE, aceF, lpdA in pseudo-operon configuration..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12630)
  AUTHORS   Xu P, Gu Q, Wang W, Wong L, Bower AG, Collins CH, Koffas MA
  TITLE     Modular optimization of multi-gene pathways for fatty acids 
            production in E. coli.
  JOURNAL   Nat Commun. 2013;4:1409. doi: 10.1038/ncomms2425.
  PUBMED    23361000
REFERENCE   2  (bases 1 to 12630)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2013"; volume: "4:1409. doi"; pages: " 
            10.1038/ncomms2425"
COMMENT     Sequence Label: pCDM4-GLY
FEATURES             Location/Qualifiers
     source          1..12630
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     519..538
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(558..635)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     841..860
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        951..969
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    970..994
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             1009..1031
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             2215..2259
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     promoter        2297..2315
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    2316..2340
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             2355..2377
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             3393..3437
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     promoter        3475..3493
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    3494..3518
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             3533..3555
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             6243..6287
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     promoter        6325..6343
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    6344..6368
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             6383..6405
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             8318..8362
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     promoter        8400..8418
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    8419..8443
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             8458..8480
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             9925..9969
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     primer_bind     complement(10008..10026)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      10022..10069
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(10226..11017)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     promoter        complement(11018..11109)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(11157..11895)
                     /direction=LEFT
                     /label=CloDF13 ori
                     /note="Plasmids containing the CloDF13 (CDF) origin of 
                     replication can be propagated in E. coli cells that contain
                     additional plasmids with compatible origins."
     CDS             complement(join(12105..12630,1..557))
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
ORIGIN
        1 gctaacagcg cgatttgctg gtgacccaat gcgaccagat gctccacgcc cagtcgcgta
       61 ccgtcttcat gggagaaaat aatactgttg atgggtgtct ggtcagagac atcaagaaat
      121 aacgccggaa cattagtgca ggcagcttcc acagcaatgg catcctggtc atccagcgga
      181 tagttaatga tcagcccact gacgcgttgc gcgagaagat tgtgcaccgc cgctttacag
      241 gcttcgacgc cgcttcgttc taccatcgac accaccacgc tggcacccag ttgatcggcg
      301 cgagatttaa tcgccgcgac aatttgcgac ggcgcgtgca gggccagact ggaggtggca
      361 acgccaatca gcaacgactg tttgcccgcc agttgttgtg ccacgcggtt gggaatgtaa
      421 ttcagctccg ccatcgccgc ttccactttt tcccgcgttt tcgcagaaac gtggctggcc
      481 tggttcacca cgcgggaaac ggtctgataa gagacaccgg catactctgc gacatcgtat
      541 aacgttactg gtttcacatt caccaccctg aattgactct cttccgggcg ctatcatgcc
      601 ataccgcgaa aggttttgcg ccattcgatg gtgtccggga tctcgacgct ctcccttatg
      661 cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc
      721 aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac
      781 catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc
      841 ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac
      901 gatgcgtccg gcgtagccta ggatcgagat cgatctcgat cccgcgaaat taatacgact
      961 cactataggg gaattgtgag cggataacaa ttcccctcta gaaataattt tgtttaactt
     1021 taagaaggag atatacatat gtctgtaatt aagatgaccg atctggatct tgctgggaaa
     1081 cgtgtattta tccgtgcgga tctgaacgta ccagtaaaag acgggaaagt aaccagcgac
     1141 gcgcgtatcc gtgcttctct gccgaccatc gaactggccc tgaaacaagg cgcaaaagtg
     1201 atggtaactt cccacctggg tcgtcctacc gaaggcgagt acaacgaaga attctctctg
     1261 ctgccggttg ttaactacct gaaagacaaa ctgtctaacc cggttcgtct ggttaaagat
     1321 tacctcgacg gcgttgacgt tgctgaaggt gaactggttg ttctggaaaa cgttcgcttc
     1381 aacaaaggcg agaagaaaga cgacgaaacc ctgtccaaaa aatacgctgc actgtgtgac
     1441 gtgttcgtaa tggacgcatt cggtactgct caccgcgcgc aggcttctac tcacggtatc
     1501 ggtaaattcg ctgacgttgc gtgcgcaggc ccgctgctgg cagctgaact ggacgcgctg
     1561 ggtaaagcac tgaaagaacc tgctcgcccg atggtggcta tcgttggtgg ttctaaagta
     1621 tctaccaaac tgaccgttct ggactccctg tctaaaatcg ctgaccagct gattgttggt
     1681 ggtggtatcg ctaacacctt tatcgcggca caaggccacg atgtgggtaa atccctgtac
     1741 gaagctgacc tggttgacga agctaaacgt ctgctgacca cctgcaacat cccggttccg
     1801 tctgatgttc gcgtagcaac cgagttctct gaaactgcac cggctaccct gaaatctgtt
     1861 aacgatgtga aagctgacga gcagatcctg gatatcggtg atgcttccgc tcaggaactg
     1921 gctgaaatcc tgaagaatgc gaaaaccatt ctgtggaacg gtccggttgg cgtgttcgaa
     1981 ttcccgaact tccgcaaagg tactgaaatc gtggctaacg ctatcgcaga cagcgaagcg
     2041 ttctccatcg ctggcggcgg cgacactctg gcagcaatcg acctgttcgg cattgctgac
     2101 aaaatctcct acatctccac tggcggcggc gcattcctcg aattcgtgga aggtaaagta
     2161 ctgcctgcag tagcgatgct cgaagagcgc gctaagaagt aactcgagtc tggtaaagaa
     2221 accgctgctg cgaaatttga acgccagcac atggactcgt ctactaggat cgagatcgat
     2281 ctcgatcccg cgaaattaat acgactcact ataggggaat tgtgagcgga taacaattcc
     2341 cctctagaaa taattttgtt taactttaag aaggagatat acatatgact atcaaagtag
     2401 gtatcaacgg ttttggccgt atcggtcgca ttgttttccg tgctgctcag aaacgttctg
     2461 acatcgagat cgttgcaatc aacgacctgt tagacgctga ttacatggca tacatgctga
     2521 aatatgactc cactcacggc cgtttcgacg gtaccgttga agtgaaagac ggtcatctga
     2581 tcgttaacgg taaaaaaatc cgtgttaccg ctgaacgtga tccggctaac ctgaaatggg
     2641 acgaagttgg tgttgacgtt gtcgctgaag caactggtct gttcctgact gacgaaactg
     2701 ctcgtaaaca catcaccgct ggtgcgaaga aagtggttat gactggtccg tctaaagaca
     2761 acactccgat gttcgttaaa ggcgctaact tcgacaaata tgctggccag gacatcgttt
     2821 ccaacgcttc ctgcaccacc aactgcctgg ctccgctggc taaagttatc aacgataact
     2881 tcggcatcat cgaaggtctg atgaccaccg ttcacgctac taccgctact cagaaaaccg
     2941 ttgatggccc gtctcacaaa gactggcgcg gcggccgcgg cgcttcccag aacatcatcc
     3001 cgtcctctac cggtgctgct aaagctgtag gtaaagtact gccagaactg aatggcaaac
     3061 tgactggtat ggcgttccgc gttccgaccc cgaacgtatc tgtagttgac ctgaccgttc
     3121 gtctggaaaa agctgcaact tacgagcaga tcaaagctgc cgttaaagct gctgctgaag
     3181 gcgaaatgaa aggcgttctg ggctacaccg aagatgacgt agtatctacc gatttcaacg
     3241 gcgaagtttg cacttccgtg ttcgatgcta aagctggtat cgctctgaac gacaacttcg
     3301 tgaaactggt atcctggtac gacaacgaaa ccggttactc caacaaagtt ctggacctga
     3361 tcgctcacat ctccaaataa ctcgagtctg gtaaagaaac cgctgctgcg aaatttgaac
     3421 gccagcacat ggactcgtct actaggatcg agatcgatct cgatcccgcg aaattaatac
     3481 gactcactat aggggaattg tgagcggata acaattcccc tctagaaata attttgttta
     3541 actttaagaa ggagatatac ataatgtcag aacgtttccc aaatgacgtg gatccgatcg
     3601 aaactcgcga ctggctccag gcgatcgaat cggtcatccg tgaagaaggt gttgagcgtg
     3661 ctcagtatct gatcgaccaa ctgcttgctg aagcccgcaa aggcggtgta aacgtagccg
     3721 caggcacagg tatcagcaac tacatcaaca ccatccccgt tgaagaacaa ccggagtatc
     3781 cgggtaatct ggaactggaa cgccgtattc gttcagctat ccgctggaac gccatcatga
     3841 cggtgctgcg tgcgtcgaaa aaagacctcg aactgggcgg ccatatggcg tccttccagt
     3901 cttccgcaac catttatgat gtgtgcttta accacttctt ccgtgcacgc aacgagcagg
     3961 atggcggcga cctggtttac ttccagggcc acatctcccc gggcgtgtac gctcgtgctt
     4021 tcctggaagg tcgtctgact caggagcagc tggataactt ccgtcaggaa gttcacggca
     4081 atggcctctc ttcctatccg cacccgaaac tgatgccgga attctggcag ttcccgaccg
     4141 tatctatggg tctgggtccg attggtgcta tttaccaggc taaattcctg aaatatctgg
     4201 aacaccgtgg cctgaaagat acctctaaac aaaccgttta cgcgttcctc ggtgacggtg
     4261 aaatggacga accggaatcc aaaggtgcga tcaccatcgc tacccgtgaa aaactggata
     4321 acctggtctt cgttatcaac tgtaacctgc agcgtcttga cggcccggtc accggtaacg
     4381 gcaagatcat caacgaactg gaaggcatct tcgaaggtgc tggctggaac gtgatcaaag
     4441 tgatgtgggg tagccgttgg gatgaactgc tgcgtaagga taccagcggt aaactgatcc
     4501 agctgatgaa cgaaaccgtt gacggcgact accagacctt caaatcgaaa gatggtgcgt
     4561 acgttcgtga acacttcttc ggtaaatatc ctgaaaccgc agcactggtt gcagactgga
     4621 ctgacgagca gatctgggca ctgaaccgtg gtggtcacga tccgaagaaa atctacgctg
     4681 cattcaagaa agcgcaggaa accaaaggca aagcgacagt aatccttgct cataccatta
     4741 aaggttacgg catgggcgac gcggctgaag gtaaaaacat cgcgcaccag gttaagaaaa
     4801 tgaacatgga cggtgtgcgt catatccgcg accgtttcaa tgtgccggtg tctgatgcag
     4861 atatcgaaaa actgccgtac atcaccttcc cggaaggttc tgaagagcat acctatctgc
     4921 acgctcagcg tcagaaactg cacggttatc tgccaagccg tcagccgaac ttcaccgaga
     4981 agcttgagct gccgagcctg caagacttcg gcgcgctgtt ggaagagcag agcaaagaga
     5041 tctctaccac tatcgctttc gttcgtgctc tgaacgtgat gctgaagaac aagtcgatca
     5101 aagatcgtct ggtaccgatc atcgccgacg aagcgcgtac tttcggtatg gaaggtctgt
     5161 tccgtcagat tggtatttac agcccgaacg gtcagcagta caccccgcag gaccgcgagc
     5221 aggttgctta ctataaagaa gacgagaaag gtcagattct gcaggaaggg atcaacgagc
     5281 tgggcgcagg ttgttcctgg ctggcagcgg cgacctctta cagcaccaac aatctgccga
     5341 tgatcccgtt ctacatctat tactcgatgt tcggcttcca gcgtattggc gatctgtgct
     5401 gggcggctgg cgaccagcaa gcgcgtggct tcctgatcgg cggtacttcc ggtcgtacca
     5461 ccctgaacgg cgaaggtctg cagcacgaag atggtcacag ccacattcag tcgctgacta
     5521 tcccgaactg tatctcttac gacccggctt acgcttacga agttgctgtc atcatgcatg
     5581 acggtctgga gcgtatgtac ggtgaaaaac aagagaacgt ttactactac atcactacgc
     5641 tgaacgaaaa ctaccacatg ccggcaatgc cggaaggtgc tgaggaaggt atccgtaaag
     5701 gtatctacaa actcgaaact attgaaggta gcaaaggtaa agttcagctg ctcggctccg
     5761 gttctatcct gcgtcacgtc cgtgaagcag ctgagatcct ggcgaaagat tacggcgtag
     5821 gttctgacgt ttatagcgtg acctccttca ccgagctggc gcgtgatggt caggattgtg
     5881 aacgctggaa catgctgcac ccgctggaaa ctccgcgcgt tccgtatatc gctcaggtga
     5941 tgaacgacgc tccggcagtg gcatctaccg actatatgaa actgttcgct gagcaggtcc
     6001 gtacttacgt accggctgac gactaccgcg tactgggtac tgatggcttc ggtcgttccg
     6061 acagccgtga gaacctgcgt caccacttcg aagttgatgc ttcttatgtc gtggttgcgg
     6121 cgctgggcga actggctaaa cgtggcgaaa tcgataagaa agtggttgct gacgcaatcg
     6181 ccaaattcaa catcgatgca gataaagtta acccgcgtct ggcgtaatag ctcgagtctg
     6241 gtaaagaaac cgctgctgcg aaatttgaac gccagcacat ggactcgtct actaggatcg
     6301 agatcgatct cgatcccgcg aaattaatac gactcactat aggggaattg tgagcggata
     6361 acaattcccc tctagaaata attttgttta actttaagaa ggagatatac atatggctat
     6421 cgaaatcaaa gtaccggaca tcggggctga tgaagttgaa atcaccgaga tcctggtcaa
     6481 agtgggcgac aaagttgaag ccgaacagtc gctgatcacc gtagaaggcg acaaagcctc
     6541 tatggaagtt ccgtctccgc aggcgggtat cgttaaagag atcaaagtct ctgttggcga
     6601 taaaacccag accggcgcac tgattatgat tttcgattcc gccgacggtg cagcagacgc
     6661 tgcacctgct caggcagaag agaagaaaga agcagctccg gcagcagcac cagcggctgc
     6721 ggcggcaaaa gacgttaacg ttccggatat cggcagcgac gaagttgaag tgaccgaaat
     6781 cctggtgaaa gttggcgata aagttgaagc tgaacagtcg ctgatcaccg tagaaggcga
     6841 caaggcttct atggaagttc cggctccgtt tgctggcacc gtgaaagaga tcaaagtgaa
     6901 cgtgggtgac aaagtgtcta ccggctcgct gattatggtc ttcgaagtcg cgggtgaagc
     6961 aggcgcggca gctccggccg ctaaacagga agcagctccg gcagcggccc ctgcaccagc
     7021 ggctggcgtg aaagaagtta acgttccgga tatcggcggt gacgaagttg aagtgactga
     7081 agtgatggtg aaagtgggcg acaaagttgc cgctgaacag tcactgatca ccgtagaagg
     7141 cgacaaagct tctatggaag ttccggcgcc gtttgcaggc gtcgtgaagg aactgaaagt
     7201 caacgttggc gataaagtga aaactggctc gctgattatg atcttcgaag ttgaaggcgc
     7261 agcgcctgcg gcagctcctg cgaaacagga agcggcagcg ccggcaccgg cagcaaaagc
     7321 tgaagccccg gcagcagcac cagctgcgaa agcggaaggc aaatctgaat ttgctgaaaa
     7381 cgacgcttat gttcacgcga ctccgctgat ccgccgtctg gcacgcgagt ttggtgttaa
     7441 ccttgcgaaa gtgaagggca ctggccgtaa aggtcgtatc ctgcgcgaag acgttcaggc
     7501 ttacgtgaaa gaagctatca aacgtgcaga agcagctccg gcagcgactg gcggtggtat
     7561 ccctggcatg ctgccgtggc cgaaggtgga cttcagcaag tttggtgaaa tcgaagaagt
     7621 ggaactgggc cgcatccaga aaatctctgg tgcgaacctg agccgtaact gggtaatgat
     7681 cccgcatgtt actcacttcg acaaaaccga tatcaccgag ttggaagcgt tccgtaaaca
     7741 gcagaacgaa gaagcggcga aacgtaagct ggatgtgaag atcaccccgg ttgtcttcat
     7801 catgaaagcc gttgctgcag ctcttgagca gatgcctcgc ttcaatagtt cgctgtcgga
     7861 agacggtcag cgtctgaccc tgaagaaata catcaacatc ggtgtggcgg tggatacccc
     7921 gaacggtctg gttgttccgg tattcaaaga cgtcaacaag aaaggcatca tcgagctgtc
     7981 tcgcgagctg atgactattt ctaagaaagc gcgtgacggt aagctgactg cgggcgaaat
     8041 gcagggcggt tgcttcacca tctccagcat cggcggcctg ggtactaccc acttcgcgcc
     8101 gattgtgaac gcgccggaag tggctatcct cggcgtttcc aagtccgcga tggagccggt
     8161 gtggaatggt aaagagttcg tgccgcgtct gatgctgccg atttctctct ccttcgacca
     8221 ccgcgtgatc gacggtgctg atggtgcccg tttcattacc atcattaaca acacgctgtc
     8281 tgacattcgc cgtctggtga tgtaactcga gtctggtaaa gaaaccgctg ctgcgaaatt
     8341 tgaacgccag cacatggact cgtctactag gatcgagatc gatctcgatc ccgcgaaatt
     8401 aatacgactc actatagggg aattgtgagc ggataacaat tcccctctag aaataatttt
     8461 gtttaacttt aagaaggaga tatacatatg agtactgaaa tcaaaactca ggtcgtggta
     8521 cttggggcag gccccgcagg ttactccgct gccttccgtt gcgctgattt aggtctggaa
     8581 accgtaatcg tagaacgtta caacaccctt ggcggtgttt gcctgaacgt cggctgtatc
     8641 ccttctaaag cactgctgca cgtagcaaaa gttatcgaag aagccaaagc gctggctgaa
     8701 cacggtatcg tcttcggcga accgaaaacc gatatcgaca agattcgtac ctggaaagag
     8761 aaagtgatca atcagctgac cggtggtctg gctggtatgg cgaaaggccg caaagtcaaa
     8821 gtggtcaacg gtctgggtaa attcaccggg gctaacaccc tggaagttga aggtgagaac
     8881 ggcaaaaccg tgatcaactt cgacaacgcg atcattgcag cgggttctcg cccgatccaa
     8941 ctgccgttta ttccgcatga agatccgcgt atctgggact ccactgacgc gctggaactg
     9001 aaagaagtac cagaacgcct gctggtaatg ggtggcggta tcatcggtct ggaaatgggc
     9061 accgtttacc acgcgctggg ttcacagatt gacgtggttg aaatgttcga ccaggttatc
     9121 ccggcagctg acaaagacat cgttaaagtc ttcaccaagc gtatcagcaa gaaattcaac
     9181 ctgatgctgg aaaccaaagt taccgccgtt gaagcgaaag aagacggcat ttatgtgacg
     9241 atggaaggca aaaaagcacc cgctgaaccg cagcgttacg acgccgtgct ggtagcgatt
     9301 ggtcgtgtgc cgaacggtaa aaacctcgac gcaggcaaag caggcgtgga agttgacgac
     9361 cgtggtttca tccgcgttga caaacagctg cgtaccaacg taccgcacat ctttgctatc
     9421 ggcgatatcg tcggtcaacc gatgctggca cacaaaggtg ttcacgaagg tcacgttgcc
     9481 gctgaagtta tcgccggtaa gaaacactac ttcgatccga aagttatccc gtccatcgcc
     9541 tataccgaac cagaagttgc atgggtgggt ctgactgaga aagaagcgaa agagaaaggc
     9601 atcagctatg aaaccgccac cttcccgtgg gctgcttctg gtcgtgctat cgcttccgac
     9661 tgcgcagacg gtatgaccaa gctgattttc gacaaagaat ctcaccgtgt gatcggtggt
     9721 gcgattgtcg gtactaacgg cggcgagctg ctgggtgaaa tcggcctggc aatcgaaatg
     9781 ggttgtgatg ctgaagacat cgcactgacc atccacgcgc acccgactct gcacgagtct
     9841 gtgggcctgg cggcagaagt gttcgaaggt agcattaccg acctgccgaa cccgaaagcg
     9901 aagaagaagt aactcgagtc tggtaaagaa accgctgctg cgaaatttga acgccagcac
     9961 atggactcgt ctactagtcg cagcttaatt aacctaaact gctgccaccg ctgagcaata
    10021 actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tagcgaaagg
    10081 aggagtcgac actgcttccg gtagtcaata aaccggtaaa ccagcaatag acataagcgg
    10141 ctatttaacg accctgccct gaaccgacga ccgggtcatc gtggccggat cttgcggccc
    10201 ctcggcttga acgaattgtt agacattatt tgccgactac cttggtgatc tcgcctttca
    10261 cgtagtggac aaattcttcc aactgatctg cgcgcgaggc caagcgatct tcttcttgtc
    10321 caagataagc ctgtctagct tcaagtatga cgggctgata ctgggccggc aggcgctcca
    10381 ttgcccagtc ggcagcgaca tccttcggcg cgattttgcc ggttactgcg ctgtaccaaa
    10441 tgcgggacaa cgtaagcact acatttcgct catcgccagc ccagtcgggc ggcgagttcc
    10501 atagcgttaa ggtttcattt agcgcctcaa atagatcctg ttcaggaacc ggatcaaaga
    10561 gttcctccgc cgctggacct accaaggcaa cgctatgttc tcttgctttt gtcagcaaga
    10621 tagccagatc aatgtcgatc gtggctggct cgaagatacc tgcaagaatg tcattgcgct
    10681 gccattctcc aaattgcagt tcgcgcttag ctggataacg ccacggaatg atgtcgtcgt
    10741 gcacaacaat ggtgacttct acagcgcgga gaatctcgct ctctccaggg gaagccgaag
    10801 tttccaaaag gtcgttgatc aaagctcgcc gcgttgtttc atcaagcctt acggtcaccg
    10861 taaccagcaa atcaatatca ctgtgtggct tcaggccgcc atccactgcg gagccgtaca
    10921 aatgtacggc cagcaacgtc ggttcgagat ggcgctcgat gacgccaact acctctgata
    10981 gttgagtcga tacttcggcg atcaccgctt ccctcatact cttccttttt caatattatt
    11041 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
    11101 ataaacaaat agccagctca ctcggtcgct acgctccggg cgtgagactg cggcgggcgc
    11161 tgcggacaca tacaaagtta cccacagatt ccgtggataa gcaggggact aacatgtgag
    11221 gcaaaacagc agggccgcgc cggtggcgtt tttccatagg ctccgccctc ctgccagagt
    11281 tcacataaac agacgctttt ccggtgcatc tgtgggagcc gtgaggctca accatgaatc
    11341 tgacagtacg ggcgaaaccc gacaggactt aaagatcccc accgtttccg gcgggtcgct
    11401 ccctcttgcg ctctcctgtt ccgaccctgc cgtttaccgg atacctgttc cgcctttctc
    11461 ccttacggga agtgtggcgc tttctcatag ctcacacact ggtatctcgg ctcggtgtag
    11521 gtcgttcgct ccaagctggg ctgtaagcaa gaactccccg ttcagcccga ctgctgcgcc
    11581 ttatccggta actgttcact tgagtccaac ccggaaaagc acggtaaaac gccactggca
    11641 gcagccattg gtaactggga gttcgcagag gatttgttta gctaaacacg cggttgctct
    11701 tgaagtgtgc gccaaagtcc ggctacactg gaaggacaga tttggttgct gtgctctgcg
    11761 aaagccagtt accacggtta agcagttccc caactgactt aaccttcgat caaaccacct
    11821 ccccaggtgg ttttttcgtt tacagggcaa aagattacgc gcagaaaaaa aggatctcaa
    11881 gaagatcctt tgatcttttc tactgaaccg ctctagattt cagtgcaatt tatctcttca
    11941 aatgtagcac ctgaagtcag ccccatacga tataagttgt aattctcatg ttagtcatgc
    12001 cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgagatc
    12061 ccggtgccta atgagtgagc taacttacat taattgcgtt gcgctcactg cccgctttcc
    12121 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
    12181 gtttgcgtat tgggcgccag ggtggttttt cttttcacca gtgagacggg caacagctga
    12241 ttgcccttca ccgcctggcc ctgagagagt tgcagcaagc ggtccacgct ggtttgcccc
    12301 agcaggcgaa aatcctgttt gatggtggtt aacggcggga tataacatga gctgtcttcg
    12361 gtatcgtcgt atcccactac cgagatgtcc gcaccaacgc gcagcccgga ctcggtaatg
    12421 gcgcgcattg cgcccagcgc catctgatcg ttggcaacca gcatcgcagt gggaacgatg
    12481 ccctcattca gcatttgcat ggtttgttga aaaccggaca tggcactcca gtcgccttcc
    12541 cgttccgcta tcggctgaat ttgattgcga gtgagatatt tatgccagcc agccagacgc
    12601 agacgcgccg agacagaact taatgggccc
//
