LOCUS       sequence-74625-e       10152 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes CnfatB2, fabA, fabH, fabG, fabI in pseudo-operon 
            configuration.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10152)
  AUTHORS   Xu P, Gu Q, Wang W, Wong L, Bower AG, Collins CH, Koffas MA
  TITLE     Modular optimization of multi-gene pathways for fatty acids 
            production in E. coli.
  JOURNAL   Nat Commun. 2013;4:1409. doi: 10.1038/ncomms2425.
  PUBMED    23361000
REFERENCE   2  (bases 1 to 10152)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2013"; volume: "4:1409. doi"; pages: " 
            10.1038/ncomms2425"
COMMENT     Sequence Label: pETM6-FAS
FEATURES             Location/Qualifiers
     source          1..10152
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    3..27
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             42..64
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             1362..1406
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     promoter        1444..1462
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1463..1487
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             1502..1524
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             2063..2107
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     promoter        2145..2163
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    2164..2188
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             2203..2225
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             3199..3243
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     promoter        3281..3299
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    3300..3324
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             3339..3361
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             4116..4160
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     promoter        4198..4216
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    4217..4241
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             4256..4278
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             5087..5131
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     primer_bind     complement(5170..5188)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      5184..5231
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      5275..5730
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     5572..5593
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(join(5845..6636,6637..6705))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6468..6487
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6706..6798)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      6879..7467
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7368..7387
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7621..7638
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    7653..7795
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(7721..7740)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7881..7903
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(7897..8088)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(8660..9742)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     9704..9723
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(9743..9820)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     10026..10045
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        join(10136..10152,1..2)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ggggaattgt gagcggataa caattcccct ctagaaataa ttttgtttaa ctttaagaag
       61 gagatataca tatggttgcg tctattgccg cctcagcctt cttcccgacc ccgtcaagca
      121 gcagttcagc agcatccgct aaagcctcta aaaccattgg cgaaggcccg ggttctctgg
      181 atgttcgtgg cattgtcgca aaaccgacca gctctagtgc ggccatgcag gaaaaagtta
      241 aagtccaacc ggtgccgaaa atcaacggcg ccaaagtggg tctgaaagtt gaaacccaga
      301 aagcagatga agaatcctca ccgtcgagcg ctccgcgtac gttttataat caactgccgg
      361 actggagcgt gctgctggca gctgttacca cgattttcct ggcggccgaa aaacagtgga
      421 ccctgctgga ttggaaaccg cgtcgcccgg atatgctggc ggacgcattt ggcctgggta
      481 aaattgtcca ggatggcctg gtgttcaaac aaaacttctc catccgttca tacgaaatcg
      541 gtgcagaccg caccgcgagc atcgaaacgc tgatgaacca tctgcaggaa accgcgctga
      601 atcacgtgaa atctgcaggt ctgatgggtg atggtttcgg tgcaaccccg gaaatgagta
      661 aacgtaacct gatttgggtg gttacgaaaa tgcgtgttct gatcgaacgc tacccgagct
      721 ggggcgatgt cgtggaagtg gatacctggg tgggtccgac gggcaaaaat ggtatgcgtc
      781 gcgattggca tgtgcgtgac caccgctccg gtcagaccat tctgcgcgca acgtcagtgt
      841 gggttatgat gaacaaaaat acccgtaaac tgtccaaagt tccggaagaa gtccgcgctg
      901 aaattggtcc gtattttgtt gaacgtgcag ctatcgtcga tgaagacagt cgcaaactgc
      961 cgaaactgga tgaagacacc acggattata tcaaaaaagg cctgaccccg cgttggggtg
     1021 atctggacgt taaccagcat gtcaacaatg tgaaatacat tggctggatc ctggaatcgg
     1081 cgccgattag catcctggaa aatcacgaac tggcctctat gagtctggaa taccgtcgcg
     1141 aatgcggtcg tgattctgtg ctgcaaagtc tgaccgccgt ttcgaatgat ctgacggacg
     1201 gcctggtcga aagcggtatt gaatgccagc atctgctgca actggaatgt ggcaccgaac
     1261 tggttaaagg tcgcacggaa tggcgtccga aacacagtcc ggctctgggc aatatgggtc
     1321 cgaccccggg cggttcagcg tgaggtaccc tcgagtctgg taaagaaacc gctgctgcga
     1381 aatttgaacg ccagcacatg gactcgtcta ctaggatcga gatcgatctc gatcccgcga
     1441 aattaatacg actcactata ggggaattgt gagcggataa caattcccct ctagaaataa
     1501 ttttgtttaa ctttaagaag gagatataca tatggtagat aaacgcgaat cctatacaaa
     1561 agaagacctt cttgcctctg gtcgcggtga actgtttggc gctaaaggcc cgcaattgcc
     1621 agcaccgaac atgctgatga tggaccgtgt ggtcaaaatg accgaaacgg gtggtaactt
     1681 cgacaaaggg tatgttgaag cagaactgga tatcaatccg gatctgtggt tcttcggatg
     1741 ccactttatt ggcgatccgg ttatgccggg atgcctgggc ctggacgcaa tgtggcagct
     1801 ggtagggttc tacctcggct ggctgggcgg cgaaggtaaa ggccgcgcgc tgggcgttgg
     1861 cgaagtgaaa ttcactggtc aggtactgcc gacagcgaaa aaagtgacct accgtattca
     1921 ctttaaacgc attgttaacc gtcgtctgat tatgggcctg gcggatggcg aagtgctggt
     1981 tgatggtcgt ctgatctata ccgccagcga cctgaaagtc ggtctgttcc aggatacgtc
     2041 tgccttctga ctcgagtctg gtaaagaaac cgctgctgcg aaatttgaac gccagcacat
     2101 ggactcgtct actaggatcg agatcgatct cgatcccgcg aaattaatac gactcactat
     2161 aggggaattg tgagcggata acaattcccc tctagaaata attttgttta actttaagaa
     2221 ggagatatac atatgtacac gaagattatt ggcacgggct cctacctgcc ggaacaagtc
     2281 cgcacgaatg ctgacctgga aaaaatggtt gatacctcgg atgaatggat cgttacccgt
     2341 acgggtatcc gtgaacgcca tattgcggcc ccgaacgaaa ccgtctctac gatgggcttt
     2401 gaagcagcta cgcgcgccat cgaaatggca ggtattgaaa aagatcagat cggcctgatt
     2461 gtggttgcca ccacgagtgc tacccacgca ttcccgtccg cagcatgcca gatccaatca
     2521 atgctgggta ttaagggctg cccggctttt gatgtggcag ctgcgtgtgc aggtttcacc
     2581 tatgctctgt ctgtggcgga ccaatacgtt aaaagtggtg ccgtcaagta tgcactggtc
     2641 gtgggctcag atgtgctggc acgtacctgt gatccgacgg accgcggcac cattatcatt
     2701 tttggtgatg gtgcaggtgc agcagttctg gctgcgtcgg aagaaccggg catcattagc
     2761 acgcatctgc acgcggatgg ttcgtacggc gaactgctga ccctgccgaa cgcagaccgt
     2821 gtgaacccgg aaaatagcat ccatctgacc atggccggca atgaagtctt caaagttgcc
     2881 gtcacggaac tggcacacat tgtggatgaa accctggccg caaacaatct ggatcgttcc
     2941 cagctggact ggctggttcc gcatcaagcg aatctgcgca tcatttccgc caccgcaaaa
     3001 aagctgggca tgtcaatgga taacgttgtc gtgacgctgg accgccacgg caataccagc
     3061 gctgcgtctg tgccgtgcgc tctggatgaa gcggttcgtg acggtcgcat taaaccgggc
     3121 cagctggtcc tgctggaagc gttcggcggt ggttttacct ggggttctgc tctggtgcgt
     3181 ttttaactcg agtctggtaa agaaaccgct gctgcgaaat ttgaacgcca gcacatggac
     3241 tcgtctacta ggatcgagat cgatctcgat cccgcgaaat taatacgact cactataggg
     3301 gaattgtgag cggataacaa ttcccctcta gaaataattt tgtttaactt taagaaggag
     3361 atatacatat gaattttgaa ggaaaaatcg cactggtaac cggtgcaagc cgcggaattg
     3421 gccgcgcaat tgctgaaacg ctcgcagccc gtggcgcgaa agttattggc actgcgacca
     3481 gtgaaaatgg cgctcaggcg atcagtgatt atttaggtgc caacggcaaa ggtctgatgt
     3541 tgaatgtgac cgacccggca tctatcgaat ctgttctgga aaaaattcgc gcagaatttg
     3601 gtgaagtgga tatcctggtc aataatgccg gtatcactcg tgataacctg ttaatgcgaa
     3661 tgaaagatga agagtggaac gatattatcg aaaccaacct ttcatctgtt ttccgtctgt
     3721 caaaagcggt aatgcgcgct atgatgaaaa agcgtcatgg tcgtattatc actatcggtt
     3781 ctgtggttgg taccatggga aatggcggtc aggccaacta cgctgcggcg aaagcgggct
     3841 tgatcggctt cagtaaatca ctggcgcgcg aagttgcgtc acgcggtatt actgtaaacg
     3901 ttgttgctcc gggctttatt gaaacggaca tgacacgtgc gctgagcgat gaccagcgtg
     3961 cgggtatcct ggcgcaggtt cctgcgggtc gcctcggcgg cgcacaggaa atcgccaacg
     4021 cggttgcatt cctggcatcc gacgaagcag cttacatcac gggtgaaact ttgcatgtga
     4081 acggcgggat gtacatggtc tgactcgagt ctggtaaaga aaccgctgct gcgaaatttg
     4141 aacgccagca catggactcg tctactagga tcgagatcga tctcgatccc gcgaaattaa
     4201 tacgactcac tataggggaa ttgtgagcgg ataacaattc ccctctagaa ataattttgt
     4261 ttaactttaa gaaggagata tacatatggg ttttctttcc ggtaagcgca ttctggtaac
     4321 cggtgttgcc agcaaactat ccatcgccta cggtatcgct caggcgatgc accgcgaagg
     4381 agctgaactg gcattcacct accagaacga caaactgaaa ggccgcgtag aagaatttgc
     4441 cgctcaattg ggttctgaca tcgttctgca gtgcgatgtt gcagaagatg ccagcatcga
     4501 caccatgttc gctgaactgg ggaaagtttg gccgaaattt gacggtttcg tacactctat
     4561 tggttttgca cctggcgatc agctggatgg tgactatgtt aacgccgtta cccgtgaagg
     4621 cttcaaaatt gcccacgaca tcagctccta cagcttcgtt gcaatggcaa aagcttgccg
     4681 ctccatgctg aatccgggtt ctgccctgct gaccctttcc taccttggcg ctgagcgcgc
     4741 tatcccgaac tacaacgtta tgggtctggc aaaagcgtct ctggaagcga acgtgcgcta
     4801 tatggcgaac gcgatgggtc cggaaggtgt gcgtgttaac gccatctctg ctggtccgat
     4861 ccgtactctg gcggcctccg gtatcaaaga cttccgcaaa atgctggctc attgcgaagc
     4921 cgttaccccg attcgccgta ccgttactat tgaagatgtg ggtaactctg cggcattcct
     4981 gtgctccgat ctctctgccg gtatctccgg tgaagtggtc cacgttgacg gcggtttcag
     5041 cattgctgca atgaacgaac tcgaactgaa ataactcgag tctggtaaag aaaccgctgc
     5101 tgcgaaattt gaacgccagc acatggactc gtctactagt cgcagcttaa ttaacctaaa
     5161 ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga
     5221 ggggtttttt gctagcgaaa ggaggagtcg actatatccg gattggcgaa tgggacgcgc
     5281 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
     5341 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
     5401 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt
     5461 tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc
     5521 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct
     5581 tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga
     5641 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
     5701 attttaacaa aatattaacg tttacaattt ctggcggcac gatggcatga gattatcaaa
     5761 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat
     5821 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc
     5881 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat
     5941 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc
     6001 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc
     6061 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag
     6121 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg
     6181 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg
     6241 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag
     6301 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt
     6361 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga
     6421 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc
     6481 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc
     6541 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc
     6601 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc
     6661 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca
     6721 atcatgattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     6781 tttagaaaaa taaacaaata ggtcatgacc aaaatccctt aacgtgagtt ttcgttccac
     6841 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     6901 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     6961 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     7021 actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     7081 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     7141 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     7201 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     7261 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     7321 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     7381 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     7441 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     7501 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     7561 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
     7621 agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat
     7681 ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg
     7741 catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg
     7801 acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta
     7861 cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc
     7921 gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat
     7981 gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct
     8041 tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt
     8101 gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac
     8161 gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact
     8221 ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt
     8281 taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa
     8341 cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa
     8401 gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg
     8461 ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt
     8521 cctcaacgac aggagcacga tcatgctagt catgccccgc gcccaccgga aggagctgac
     8581 tgggttgaag gctctcaagg gcatcggtcg agatcccggt gcctaatgag tgagctaact
     8641 tacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
     8701 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gccagggtgg
     8761 tttttctttt caccagtgag acgggcaaca gctgattgcc cttcaccgcc tggccctgag
     8821 agagttgcag caagcggtcc acgctggttt gccccagcag gcgaaaatcc tgtttgatgg
     8881 tggttaacgg cgggatataa catgagctgt cttcggtatc gtcgtatccc actaccgaga
     8941 tgtccgcacc aacgcgcagc ccggactcgg taatggcgcg cattgcgccc agcgccatct
     9001 gatcgttggc aaccagcatc gcagtgggaa cgatgccctc attcagcatt tgcatggttt
     9061 gttgaaaacc ggacatggca ctccagtcgc cttcccgttc cgctatcggc tgaatttgat
     9121 tgcgagtgag atatttatgc cagccagcca gacgcagacg cgccgagaca gaacttaatg
     9181 ggcccgctaa cagcgcgatt tgctggtgac ccaatgcgac cagatgctcc acgcccagtc
     9241 gcgtaccgtc ttcatgggag aaaataatac tgttgatggg tgtctggtca gagacatcaa
     9301 gaaataacgc cggaacatta gtgcaggcag cttccacagc aatggcatcc tggtcatcca
     9361 gcggatagtt aatgatcagc ccactgacgc gttgcgcgag aagattgtgc accgccgctt
     9421 tacaggcttc gacgccgctt cgttctacca tcgacaccac cacgctggca cccagttgat
     9481 cggcgcgaga tttaatcgcc gcgacaattt gcgacggcgc gtgcagggcc agactggagg
     9541 tggcaacgcc aatcagcaac gactgtttgc ccgccagttg ttgtgccacg cggttgggaa
     9601 tgtaattcag ctccgccatc gccgcttcca ctttttcccg cgttttcgca gaaacgtggc
     9661 tggcctggtt caccacgcgg gaaacggtct gataagagac accggcatac tctgcgacat
     9721 cgtataacgt tactggtttc acattcacca ccctgaattg actctcttcc gggcgctatc
     9781 atgccatacc gcgaaaggtt ttgcgccatt cgatggtgtc cgggatctcg acgctctccc
     9841 ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc
     9901 gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct
     9961 gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc
    10021 ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg
    10081 gccacgatgc gtccggcgta gcctaggatc gagatcgatc tcgatcccgc gaaattaata
    10141 cgactcacta ta
//
