LOCUS       sequence-74627-e         991 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd gen lentiviral vector for EGFP fusion; PGK driven puromycin.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 991)
  AUTHORS   Sancak Y, Peterson TR, Shaul YD, Lindquist RA, Thoreen CC, Bar-Peled
            L, Sabatini DM
  TITLE     The Rag GTPases bind raptor and mediate amino acid signaling to 
            mTORC1.
  JOURNAL   Science. 2008 Jun 13. 320(5882):1496-501.
  PUBMED    18497260
REFERENCE   2  (bases 1 to 991)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2008 Jun 13. 320(5882):1496-501."
COMMENT     Sequence Label: pLJM1-EGFP
FEATURES             Location/Qualifiers
     source          1..991
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      24..33
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(72..275)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(101..121)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     complement(564..584)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     CDS             complement(882..926)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 gaacagctcc tcgcccttgc tcaccatggt ggcgaccggt agcgctagcg gatctgacgg
       61 ttcactaaac cagctctgct tatatagacc tcccaccgta cacgcctacc gcccatttgc
      121 gtcaatgggg cggagttgtt acgacatttt ggaaagtccc gttgattttg gtgccaaaac
      181 aaactcccat tgacgtcaat ggggtggaga cttggaaatc cccgtgagtc aaaccgctat
      241 ccacgcccat tgatgtactg ccaaaaccgc atcaccatgg taatagcgat gactaatacg
      301 tagatgtact gccaagtagg aaagtcccat aaggtcatgt actgggcata atgccaggcg
      361 ggccatttac cgtcattgac gtcaataggg ggcgtacttg gcatatgata gtccatttta
      421 aaacataatt ttaaaactgc aaactaccca agaaattatt actttctacg tcacgtattt
      481 tgtactaata tctttgtgtt tacagtcaaa ttaattccaa ttatctctct aacagccttg
      541 tatcgtatat gcaaatatga aggaatcatg ggaaataggc cctcggtgaa gggggcggcc
      601 gctcgaggct agtctcgtga tcgataccgt cgagatccgt tcactaatcg aatggatctg
      661 tctctgtctc tctctccacc ttcttcttct attccttcgg gcctgtcggg tcccctcggg
      721 gttgggaggt gggtctgaaa cgataatggt gaatatccct gcctaactct attcactata
      781 gaaagtacag caaaaactat tcttaaacct accaagcctc ctactatcat tatgaataat
      841 tttatatacc acagccaatt tgttatgtta aaccaattcc acaaacttgc ccatttatct
      901 aattccaata attcttgttc attcttttct tgctggtttt gcgattcttc aattaaggag
      961 tgtattaagc ttgtgtaatt gttaatttct c
//
