LOCUS       sequence-74649-e         904 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  donor vector for cloning human Akt1 with 6xHN tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 904)
  TITLE     McKinnon Lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 904)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pDNR-hAkt1
FEATURES             Location/Qualifiers
     source          1..904
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     4..21
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    complement(33..66)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 cgcagcgagt cagtgagcga ggaagcggcc gcataacttc gtatagcata cattatacga
       61 agttatcagt cgacggtacc ggacatatgc ccgggaattc agaggagcgc gtgagcgtcg
      121 cgggagcctc gggcaccatg agcgacgtgg ctattgtgaa ggagggttgg ctgcacaaac
      181 gaggggagta catcaagacc tggcggccac gctacttcct cctcaagaat gatggcacct
      241 tcattggcta caaggagcgg ccgcaggatg tggaccaacg tgaggctccc ctcaacaact
      301 tctctgtggc gcagtgccag ctgatgaaga cggagcggcc ccggcccaac accttcatca
      361 tccgctgcct gcagtggacc actgtcatcg aacgcacctt ccatgtggag actcctgagg
      421 agcgggagga gtggacaacc gccatccaga ctgtggctga cggcctcaag aagcaggagg
      481 aggaggagat ggacttccgg tcgggctcac ccagtgacaa ctcaggggct gaagagatgg
      541 aggtgtccct ggccaagccc aagcaccgcg tgaccatgaa cgagtttgag tacctgaagc
      601 tgctgggcaa gggcactttc ggcaaggtga tcctggtgaa ggagaaggcc acaggccgct
      661 actacgccat gaagatcctc aagaaggaag tcatcgtggc caaggacgag gtggcccaca
      721 cactcaccga gaaccgcgtc ctgcagaact ccaggcaccc cttcctcaca gccctgaagt
      781 actctttcca gacccacgac cgcctctgct ttgtcatgga gtacgccaac gggggcgagc
      841 tgttcttcca cctgtcccgg gagcgtgtgt tctccgagga ccgggcccgc ttctatggcg
      901 ctga
//
