LOCUS       sequence-74660-e        9612 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9612)
  AUTHORS   Cai D, Cohen KB, Luo T, Lichtman JW, Sanes JR
  TITLE     Improved tools for the Brainbow toolbox.
  JOURNAL   Nat Methods. 2013 May 5;10(6):540-7. doi: 10.1038/nmeth.2450. Epub 
            2013 May 5.
  PUBMED    23817127
REFERENCE   2  (bases 1 to 9612)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2450"; journalName: "Nat Methods"; date: "2013-05-5- 
            5"; volume: "10"; issue: "6"; pages: "540-7"
COMMENT     Sequence Label: pCAG-Autobow
FEATURES             Location/Qualifiers
     source          1..9612
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        4..383
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        386..661
                     /label=chicken beta-actin promoter
     intron          662..1670
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1594..1616
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1678..1697
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    1737..1761
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1810..1843)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(1852..1885)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    complement(1894..1927)
                     /label=loxN
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AGGTATAC) (Shaw et al., 2021). loxN sites are 
                     compatible with each other, but incompatible with loxP or 
                     lox2272 sites."
     intron          2764..2829
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     polyA_signal    3184..3239
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     protein_bind    complement(3561..3594)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     regulatory      3604..3613
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3610..3612,3613..3615,3616..4329)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3916..3935)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4263..4284
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    4452..4573
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(4590..4623)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     CDS             4638..5342
                     /codon_start=1
                     /product="Phialidium yellow fluorescent proteim"
                     /label=PhiYFP
                     /note="mammalian codon-optimized"
                     /translation="MSSGALLFHGKIPYVVEMEGNVDGHTFSIRGKGYGDASVGKVDAQ
                     FICTTGDVPVPWSTLVTTLTYGAQCFAKYGPELKDFYKSCMPDGYVQERTITFEGDGNF
                     KTRAEVTFENGSVYNRVKLNGQGFKKDGHVLGKNLEFNFTPHCLYIWGDQANHGLKSAF
                     KICHEITGSKGDFIVADHTQMNTPIGGGPVHVPEYHHMSYHVKLSKDVTDHRDNMSLKE
                     TVRAVDCRKTYL"
     polyA_signal    5525..5646
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(5683..5716)
                     /label=loxN
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AGGTATAC) (Shaw et al., 2021). loxN sites are 
                     compatible with each other, but incompatible with loxP or 
                     lox2272 sites."
     regulatory      5732..5741
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5738..6430
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     AVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRADMAL
                     KLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARY
                     CDLPSKLGH"
     protein_bind    6449..6482
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     protein_bind    complement(6521..6545)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    6701..6756
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(7115..7131)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7115..7131)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7128..7150)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7139..7155
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7163..7169,7170..7187,7188..7193))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7208..7229
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        7288..7483
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      7334..7469
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    7489..7623
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7691..7708)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7862..8450)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7942..7961)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8621..9412,9413..9481))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9244..9263
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9482..9586)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atgggtcgag gtgagcccca cgttctgctt cactctcccc
      421 atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca
      481 gcgatggggg cggggggggg gggggcgcgc gccaggcggg gcggggcggg gcgaggggcg
      541 gggcggggcg aggcggagag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt
      601 tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc
      661 gggagtcgct gcgttgcctt cgccccgtgc cccgctccgc gccgcctcgc gccgcccgcc
      721 ccggctctga ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc
      781 gggctgtaat tagcgcttgg tttaatgacg gctcgtttct tttctgtggc tgcgtgaaag
      841 ccttaaaggg ctccgggagg gccctttgtg cgggggggag cggctcgggg ggtgcgtgcg
      901 tgtgtgtgtg cgtggggagc gccgcgtgcg gcccgcgctg cccggcggct gtgagcgctg
      961 cgggcgcggc gcggggcttt gtgcgctccg cgtgtgcgcg aggggagcgc ggccgggggc
     1021 ggtgccccgc ggtgcggggg ggctgcgagg ggaacaaagg ctgcgtgcgg ggtgtgtgcg
     1081 tgggggggtg agcagggggt gtgggcgcgg cggtcgggct gtaacccccc cctgcacccc
     1141 cctccccgag ttgctgagca cggcccggct tcgggtgcgg ggctccgtgc ggggcgtggc
     1201 gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg cggggcgggg
     1261 ccgcctcggg ccggggaggg ctcgggggag gggcgcggcg gccccggagc gccggcggct
     1321 gtcgaggcgc ggcgagccgc agccattgcc ttttatggta atcgtgcgag agggcgcagg
     1381 gacttccttt gtcccaaatc tggcggagcc gaaatctggg aggcgccgcc gcaccccctc
     1441 tagcgggcgc gggcgaagcg gtgcggcgcc ggcaggaagg aaatgggcgg ggagggcctt
     1501 cgtgcgtcgc cgcgccgccg tccccttctc catctccagc ctcggggctg ccgcaggggg
     1561 acggctgcct tcggggggga cggggcaggg cggggttcgg cttctggcgt gtgaccggcg
     1621 gctctagagc ctctgctaac catgttcatg ccttcttctt tttcctacag ctcctgggca
     1681 acgtgctggt tattgtgctg tctcatcatt ttggcaaaga attcctcgag gatatcacaa
     1741 gtttgtacaa aaaagcaggc tttaaaggaa cgcacgtaac tataacggtc ctaaggtagc
     1801 gaacctagga taacttcgta tagcatacat tatacgaagt tatcggcgcg cataacttcg
     1861 tataggatac tttatacgaa gttatcaagc gctataactt cgtatagtat accttatacg
     1921 aagttatctg atgcaatggc caatttactg accgtacacc aaaatttgcc tgcattaccg
     1981 gtcgatgcaa cgagtgatga ggttcgcaag aacctgatgg acatgttcag ggatcgccag
     2041 gcgttttctg agcatacctg gaaaatgctt ctgtccgttt gccggtcgtg ggcggcatgg
     2101 tgcaagttga ataaccggaa atggtttccc gcagaacctg aagatgttcg cgattatctt
     2161 ctatatcttc aggcgcgcgg tctggcagta aaaactatcc agcaacattt gggccagcta
     2221 aacatgcttc atcgtcggtc cgggctgcca cgaccaagtg acagcaatgc tgtttcactg
     2281 gttatgcggc ggatccgaaa agaaaacgtt gatgccggtg aacgtgcaaa acaggctcta
     2341 gcgttcgaac gcactgattt cgaccaggtt cgttcactca tggaaaatag cgatcgctgc
     2401 caggatatac gtaatctggc atttctgggg attgcttata acaccctgtt acgtatagcc
     2461 gaaattgcca ggatcagggt taaagatatc tcacgtactg acggtgggag aatgttaatc
     2521 catattggca gaacgaaaac gctggttagc accgcaggtg tagagaaggc acttagcctg
     2581 ggggtaacta aactggtcga gcgatggatt tccgtctctg gtgtagctga tgatccgaat
     2641 aactacctgt tttgccgggt cagaaaaaat ggtgttgccg cgccatctgc caccagccag
     2701 ctatcaactc gcgccctgga agggattttt gaagcaactc atcgattgat ttacggcgct
     2761 aaggtaaata taaaattttt aagtgtataa tgtgttaaac tactgattct aattgtttgt
     2821 gtattttagg atgactctgg tcagagatac ctggcctggt ctggacacag tgcccgtgtc
     2881 ggagccgcgc gagatatggc ccgcgctgga gtttcaatac cggagatcat gcaagctggt
     2941 ggctggacca atgtaaatat tgtcatgaac tatatccgta acctggatag tgaaacaggg
     3001 gcaatggtgc gcctgctgga agatggcgat taagcggccg cactcctcag gtgcaggctg
     3061 cctatcagaa ggtggtggct ggtgtggcca atgccctggc tcacaaatac cactgagatc
     3121 tttttccctc tgccaaaaat tatggggaca tcatgaagcc ccttgagcat ctgacttctg
     3181 gctaataaag gaaatttatt ttcattgcaa tagtgtgttg gaattttttg tgtctctcac
     3241 tcggaaggac atatgggagg gcaaatcatt taaaacatca gaatgagtat ttggtttaga
     3301 gtttggcaac atatgcccat atgctggctg ccatgaacaa aggttggcta taaagaggtc
     3361 atcagtatat gaaacagccc cctgctgtcc attccttatt ccatagaaaa gccttgactt
     3421 gaggttagat tttttttata ttttgttttg tgttattttt ttctttaaca tccctaaaat
     3481 tttccttaca tgttttacta gccagatttt tcctcctctc ctgactactc ccagtcatag
     3541 ctgtccctct tctcgctagc ataacttcgt atagcataca ttatacgaag ttatctgaac
     3601 gttgccacca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc
     3661 gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat
     3721 gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc
     3781 tggcccaccc tcgtgaccac cctgacctgg ggcgtgcagt gcttcgcccg ctaccccgac
     3841 cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc
     3901 accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc
     3961 gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc
     4021 ctggggcaca agctggagta caacgccatc agcgacaacg tctatatcac cgccgacaag
     4081 cagaagaacg gcatcaaggc caacttcaag atccgccaca acatcgagga cggcagcgtg
     4141 cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc
     4201 gacaaccact acctgagcac ccagtccaag ctgagcaaag accccaacga gaagcgcgat
     4261 cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg
     4321 tacaagtaaa gcggccgcga ctctagatca taatcagcca taccacattt gtagaggttt
     4381 tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa
     4441 ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     4501 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     4561 tcaatgtatc ttaaggcgtg tttaaacata taacttcgta taggatactt tatacgaagt
     4621 tatcgcgtac ggccaccatg agcagcggcg ccctgctgtt ccacggcaag atcccctacg
     4681 tggtggagat ggagggcaat gtggatggcc acaccttcag catccgcggc aagggctacg
     4741 gcgatgccag cgtgggcaag gtggatgccc agttcatctg caccaccggc gatgtgcccg
     4801 tgccctggag caccctggtg accaccctga cctacggcgc ccagtgcttc gccaagtacg
     4861 gccccgagct gaaggatttc tacaagagct gcatgcccga tggctacgtg caggagcgca
     4921 ccatcacctt cgagggtgat ggcaatttca agacccgcgc cgaggtgacc ttcgagaatg
     4981 gcagcgtgta caatcgcgtg aagctgaatg gccagggctt caagaaggat ggccacgtgc
     5041 tgggcaagaa tctggagttc aatttcaccc cccactgcct gtacatctgg ggcgatcagg
     5101 ccaatcacgg cctgaagagc gccttcaaga tctgccacga gatcaccggc agcaagggcg
     5161 atttcatcgt ggccgatcac acccagatga atacccccat cggcggcggc cccgtgcacg
     5221 tgcccgagta ccaccacatg agctaccacg tgaagctgag caaggatgtg accgatcacc
     5281 gcgataatat gagcctgaag gagaccgtgc gcgccgtgga ttgccgcaag acctacctgt
     5341 gaagcttaat tagctgagct tggactcctg ttgatagatc cagtaatgac ctcagaactc
     5401 catctggccg cgactctaga tcataatcag ccataccaca tttgtagagg ttttacttgc
     5461 tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt
     5521 tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     5581 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     5641 atcttaaggc gtgaagagct tcataatctt cgcgttcttc atataacttc gtatagtata
     5701 ccttatacga agttatcaag tatccagtac tgccaccatg gtgagcgagc tgattaagga
     5761 gaacatgcac atgaagctgt acatggaggg caccgtgaac aaccaccact tcaagtgcac
     5821 atccgagggc gaaggcaagc cctacgaggg cacccagacc atgagaatca aggcggtcga
     5881 gggcggccct ctccccttcg ccttcgacat cctggctacc agcttcatgt acggcagcaa
     5941 aaccttcatc aaccacaccc agggcatccc cgacttcttt aagcagtcct tccccgaggg
     6001 cttcacatgg gagagagtca ccacatacga agacgggggc gtgctgaccg ctacccagga
     6061 caccagcctc caggacggct gcctcatcta caacgtcaag atcagagggg tgaacttccc
     6121 atccaacggc cctgtgatgc agaagaaaac actcggctgg gaggcctcca ccgagaccct
     6181 gtaccccgct gacggcggcc tggaaggcag agccgacatg gccctgaagc tcgtgggcgg
     6241 gggccacctg atctgcaact tgaagaccac atacagatcc aagaaacccg ctaagaacct
     6301 caagatgccc ggcgtctact atgtggacag aagactggaa agaatcaagg aggccgacaa
     6361 agagacctac gtcgagcagc acgaggtggc tgtggccaga tactgcgacc tccctagcaa
     6421 actggggcac aagcttaatt aaactagtga agttcctatt ctctagaaag tataggaact
     6481 tccgtaacta taacggtcct aaggtagcga atgcatctag acccagcttt cttgtacaaa
     6541 gtggtgattc gcggccgcac tcctcaggtg caggctgcct atcagaaggt ggtggctggt
     6601 gtggccaatg ccctggctca caaataccac tgagatcttt ttccctctgc caaaaattat
     6661 ggggacatca tgaagcccct tgagcatctg acttctggct aataaaggaa atttattttc
     6721 attgcaatag tgtgttggaa ttttttgtgt ctctcactcg gaaggacata tgggagggca
     6781 aatcatttaa aacatcagaa tgagtatttg gtttagagtt tggcaacata tgccatatgc
     6841 tggctgccat gaacaaaggt ggctataaag aggtcatcag tatatgaaac agccccctgc
     6901 tgtccattcc ttattccata gaaaagcctt gacttgaggt tagatttttt ttatattttg
     6961 ttttgtgtta tttttttctt taacatccct aaaattttcc ttacatgttt tactagccag
     7021 atttttcctc ctctcctgac tactcccagt catagctgtc cctcttctct tatgaagatc
     7081 cctcgacctg cagcccaagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
     7141 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
     7201 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
     7261 cgggaaacct gtcgtgccag cggatccgca tctcaattag tcagcaacca tagtcccgcc
     7321 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
     7381 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca
     7441 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctaa cttgtttatt
     7501 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     7561 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg
     7621 atccgctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
     7681 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     7741 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     7801 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     7861 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     7921 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     7981 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     8041 gcgtggcgct ttctcaatgc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     8101 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     8161 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     8221 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     8281 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta
     8341 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     8401 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     8461 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     8521 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     8581 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     8641 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     8701 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     8761 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     8821 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     8881 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     8941 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     9001 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     9061 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     9121 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     9181 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     9241 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     9301 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     9361 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     9421 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     9481 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     9541 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     9601 aagtgccacc tg
//
