LOCUS       sequence-74664-e       15564 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15564)
  AUTHORS   Cai D, Cohen KB, Luo T, Lichtman JW, Sanes JR
  TITLE     Improved tools for the Brainbow toolbox.
  JOURNAL   Nat Methods. 2013 May 5;10(6):540-7. doi: 10.1038/nmeth.2450. Epub 
            2013 May 5.
  PUBMED    23817127
REFERENCE   2  (bases 1 to 15564)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2450"; journalName: "Nat Methods"; date: "2013-05-5- 
            5"; volume: "10"; issue: "6"; pages: "540-7"
COMMENT     Sequence Label: pThy1-Brainbow3.2
FEATURES             Location/Qualifiers
     source          1..15564
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(10..28)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        96..200
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(201..269,270..1061)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(419..438)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1232..1820
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1721..1740
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1974..1991
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6246..6270
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(6319..6352)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(6361..6394)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    complement(6403..6436)
                     /label=loxN
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AGGTATAC) (Shaw et al., 2021). loxN sites are 
                     compatible with each other, but incompatible with loxP or 
                     lox2272 sites."
     regulatory      6445..6454
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6457..6477
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6478..7182
                     /codon_start=1
                     /product="Phialidium yellow fluorescent proteim"
                     /label=PhiYFP
                     /note="mammalian codon-optimized"
                     /translation="MSSGALLFHGKIPYVVEMEGNVDGHTFSIRGKGYGDASVGKVDAQ
                     FICTTGDVPVPWSTLVTTLTAGAQCFAKYGPELKDFYKSCMPDGYVQERTITFEGDGNF
                     KTRAEVTFENGSVYNRVKLNGQGFKKDGHVLGKNLEFNFTPHCLYIWGDQANHGLKSAF
                     KICHEITGSKGDFIVADHTQMNTPIGGGPVHVPEYHHMSYHVKLSKDVTDHRDNMSLKE
                     TVRAVDCRKTYL"
     polyA_signal    7365..7486
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(7499..7532)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     regulatory      7541..7550
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7547..8254
                     /codon_start=1
                     /product="photostable monomeric orange derivative of DsRed 
                     fluorescent protein (Shaner et al., 2008)"
                     /label=mOrange2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEENNMAIIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEG
                     FQTAKLKVTKGGPLPFAWDILSPHFTYGSKAYVKHPADIPDYFKLSFPEGFKWERVMNY
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GKIKMRLKLKDGGHYTSEVKTTYKAKKPVQLPGAYIVDIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     misc_feature    8342..8930
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(8813..8824)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    9064..9185
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(9202..9235)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     regulatory      9244..9253
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(9250..9252,9253..9255,9256..9966)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(9250..9252,9253..9255,9256..9966)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(9295..9316)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(9556..9575)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     9903..9924
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    10054..10642
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(10525..10536)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    10776..10897
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(10922..10955)
                     /label=loxN
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AGGTATAC) (Shaw et al., 2021). loxN sites are 
                     compatible with each other, but incompatible with loxP or 
                     lox2272 sites."
     regulatory      10964..10973
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             10970..11665
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     AVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRADMAL
                     KLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARY
                     CDLPSKLGHR"
     misc_feature    11753..12341
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(12224..12235)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    12475..12596
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(15395..15414)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     15514..15536
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
      181 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
      241 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
      301 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
      361 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
      421 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
      481 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
      541 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
      601 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
      661 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
      721 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
      781 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
      841 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
      901 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
      961 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     1021 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     1081 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     1141 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     1201 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     1261 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     1321 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc
     1381 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     1441 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     1501 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     1561 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     1621 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     1681 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     1741 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     1801 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1861 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1921 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1981 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     2041 cgattcatta atgcaggatc gcggccgcga tccccgggcg agctcgaatt cagagaccgg
     2101 gaaccaaact agcctttaaa aaacataagt acaggagcca gcaagatggc tcagtgggta
     2161 aaggtgccta ccagcaagcc tgacagcctg agttcagtcc ccacgaacta cgtggtagga
     2221 gaggaccaac caactctgga aatctgttct gcaaacacat gctcacacac acacacacaa
     2281 atagtataaa caattttaaa tttcatttaa aaataatttg taaacaaaat cattagcaca
     2341 ggttttagaa agagcctctt ggtgacatca agttgatgct gtagatgggg tatcattcct
     2401 gaggacccaa aaccgggtct cagcctttcc ccattctgag agttctctct tttctcagcc
     2461 actagctgaa gagtagagtg gctcagcact gggctcttga gttcccaagt cctacaactg
     2521 gtcagcctga ctactaacca gccatgaaga aacaaggagt ggatgggctg agtctgctgg
     2581 gatgggagtg gagttagtaa gtggccatgg atgtaatgac cccagcaatg ctggctagaa
     2641 ggcatgcctc ctttccttgt ctggagacgg aacgggatca tcttgtactc acagaaggga
     2701 gaacattcta gctggttggg ccaaaatgtg caagttcacc tggaggtggt ggtgcatgct
     2761 tttaactcca gtactcagga ggcagggcca ggtggatctc tgtgagttca agaccagcct
     2821 gcactatgga gagagttttg ggacagccag agttacacag aaaaatcctg gtggaaaatc
     2881 tgaaagaaag agagaaagaa agaaagaaag aaagaaagaa agaaagaaag aaagaaagaa
     2941 agaaagaaag aaagaaagga agaaagaaag aaagagtggc aggcaggcag gcaggaggaa
     3001 ggaaggaagg aaggaaggaa ggaaggaagg aaggaaggaa ggaaggaagg aaggaaggaa
     3061 aataggtgcg acttcaagat ccggagttac aagcagaatg cactgtttcc ctaacagggc
     3121 caagtgtttt gagtaactga aggtgggcat gatgcctggg aagcagaaac aagccaggca
     3181 gatgcacccc ttgccttgct tccgaagggc tgcagtagca tggaaaacat ggaaaacaac
     3241 caatccattc cctttgctga tataacaggc tccaaagcca aaacctgtca ctggaggctc
     3301 aagagcagat ctccagccaa gaggcaaagg aatgggggaa gctggagggc ctccctctgg
     3361 ttatccaggc ttctgaaggt tcaagcaaag aaagggttac aaccttaaaa ggagagcgtc
     3421 ccggggtatg ggtagaagac tgctccaccc cgacccccag ggtccctaac cgtcttttcc
     3481 ctgggcgagt cagcccaatc acaggactga gagtgcctct ttagtagcag caagccactt
     3541 cggacaccca aatggaacac ctccagtcag ccctcgccga ccaccccacc ccctccatcc
     3601 ttttccctca gcctccgatt ggctgaatct agagtccctc cctgctcccc cctctctccc
     3661 cacccctggt aaaactgcgg gcttcaggct gggtgcagca actggaggcg ttggcgacca
     3721 ggaggaggct gcagctaggg gagtccaggt gagagcaggc cgacgggagg gacccgcaca
     3781 tgcaaggacc gccgcagggc gaggatgcaa gccttcccca gctacagttt tgggaaagga
     3841 taccagggcg ctcctatatg ggggcgcggg aacctgggga aagaaggtgc tcccaggtcg
     3901 aggtgggaga ggaaggcagt gcggggtcac gggctttctc cctgctaacg gacgctttcg
     3961 aagagtgggt gccggagaga accatgagga aggacatcaa ggacagcctt tggtccccaa
     4021 gctcaaatcg ctttagtggt gcgaatagag ggaggaggtg ggtggcaaac tggagggagt
     4081 ccccagcggt gacctcgtgg ctggctggtg cggggcacca ggtaagaaac gcaatgttgc
     4141 gggaggggac tgggtggcag gcgcggggga ggggaaagct agaaaggatg cgagggagcg
     4201 gaggggggag ggagcggggg aatctcaact ggtagaggaa agttaaaatg aggaaatagc
     4261 atcagggtgg ggttagccaa gccgggcctc agggaaaggg cgcaaagttt gtctgggtgt
     4321 gggcttaggt gggctgggta tgagattcgg ggcgccgaaa acactgctgc gcctctgcca
     4381 aatcacgcta cccctgtatc tagttctgct aggcttctcc agccccagcc ccaattcttt
     4441 tctcagtgtc cccttccctc ccctgaatct caagcccaca ctccctcctc cataacccac
     4501 tgttatcaaa tctaagtcat ttgccaccca acaaccatca ggaggcggaa gcagacggga
     4561 ggagtttgag atcaacttgg gctacatcac gagttccaag ctcaccaagg cttcttaagg
     4621 agaccttgtc tctaaaatta attaattaat taattaatag tcccctttct ctgccacaga
     4681 accttgggat ctggctcctg gtcgcagctc cccccacccc aggctgacat tcactgccat
     4741 agcccatccg gaaatcctag tctattcccc atggatcttg aactgcagag agaatggcag
     4801 agtggcccgc cctgtgcaaa ggatgttcct agcctaggtg gagctcgcga actcggagac
     4861 tgtgcctctc ttgggcaagg acaggctaga cagcctgccg gtgttgagct agggcactgt
     4921 ggggaaggca gagaacctgt gcagggcacg caatgaacac aggaccagaa aactgcagcc
     4981 ctaggaacac tcaagagctg gccatttgca agcatctctg gcctccgtgc ttctcactca
     5041 tgtcccatgt cttatacagg cctctgtggc acctcgcttg cctgatctca tccctagccg
     5101 ttaagctttc tgcatgactt atcacttggg gcataatgct ggatacctac cattttctta
     5161 gaccccatca aaatcctatt tgagtgtacg gttcggagaa ctccttattt atccggtaaa
     5221 tgtcttttac tctgctctca gggagctgag gcaggacatt cctgagatac attgggagag
     5281 gaatacagtt tcaataaaat aataggttgg gtggaggtac atgcctataa tgccaccact
     5341 caggaaatgg tggcagcttc gtgagtttga ggccaaccca agaaacatag tgaaaccctg
     5401 tcagtaaata agtaagcaag tatttgagta tctactatat gctagggctg acctggacat
     5461 taggggtcat cttctgaaca aactagtgct tgagggaggt atttggggtt tttgtttgtt
     5521 taatggatct gaatgagttc cagagactgg ctacacagcg atatgactga gcttaacacc
     5581 cctaaagcat acagtcagac caattagaca ataaaaggta tgtatagctt accaaataaa
     5641 aaaattgtat tttcaagaga gtgtctgtct gtgtagccct ggctgttctt gaactcactc
     5701 tgtagaccag gctggcctgg aaatccatct gcctctgcct ctctgcctct ctgcctctct
     5761 gcctctctct ctgcctctct ctgcctctct ctgcccctct ctgcccctct ctgcccctct
     5821 ctgccgccct ctgccttttg ccctctgccc tctgttctct ggcctctgcc ctctccctgc
     5881 cttctccctc tgcctctgcc tcttgagtgc tggaatcaaa ggtgtgagct ctataggtct
     5941 taagttccag aagaaacgta atgaagtcac ccagcaggag gtgctcaggg acagcaagac
     6001 acacacaccc aggacactag gctcccactt ccttggcttt ctctgagtgg caaaggacct
     6061 taggcagtgt cactccctaa gagaagggga taaagagagg ggctgaggta ttcagtcatg
     6121 tgctccgtgg atctcaagcc ctcaaggtaa atggggaccc acctgtccta ccagctggct
     6181 gacctgtagc tttccccacc acagaatcca agtcggaact cttggcacct agaggatctc
     6241 gaatcacaag tttgtacaaa aaagcaggct ttaaaggaac gcacgtaact ataacggtcc
     6301 taaggtagcg aacctaggat aacttcgtat agcatacatt atacgaagtt atcggcgcgc
     6361 ataacttcgt ataggatact ttatacgaag ttatcaagcg ctataacttc gtatagtata
     6421 ccttatacga agttatctga tatcgccacc atggctccta agaagaagag gaaggtgatg
     6481 agcagcggcg ccctgctgtt ccacggcaag atcccctacg tggtggagat ggagggcaat
     6541 gtggatggcc acaccttcag catccgcggc aagggctacg gcgatgccag cgtgggcaag
     6601 gtggatgccc agttcatctg caccaccggc gatgtgcccg tgccctggag caccctggtg
     6661 accaccctga ccgcaggcgc ccagtgcttc gccaagtacg gccccgagct gaaggatttc
     6721 tacaagagct gcatgcccga tggctacgtg caggagcgca ccatcacctt cgagggtgat
     6781 ggcaatttca agacccgcgc cgaggtgacc ttcgagaatg gcagcgtgta caatcgcgtg
     6841 aagctgaatg gccagggctt caagaaggat ggccacgtgc tgggcaagaa tctggagttc
     6901 aatttcaccc cccactgcct gtacatctgg ggcgatcagg ccaatcacgg cctgaagagc
     6961 gccttcaaga tctgccacga gatcaccggc agcaagggcg atttcatcgt ggccgatcac
     7021 acccagatga atacccccat cggcggcggc cccgtgcacg tgcccgagta ccaccacatg
     7081 agctaccacg tgaagctgag caaggatgtg accgatcacc gcgataatat gagcctgaag
     7141 gagaccgtgc gcgccgtgga ttgccgcaag acctacctgt gaagcttaat tagctgagct
     7201 tggactcctg ttgatagatc cagtaatgac ctcagaactc catctggccg cgactctaga
     7261 tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc
     7321 tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag
     7381 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     7441 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttaaggc gtgctagcat
     7501 aacttcgtat agcatacatt atacgaagtt atctaacgtt gccaccatgg tgagcaaggg
     7561 cgaggagaat aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc gcatggaggg
     7621 ctccgtgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg
     7681 ctttcagacc gctaagctga aggtgaccaa gggtggcccc ctgcccttcg cctgggacat
     7741 cctgtcccct catttcacct acggctccaa ggcctacgtg aagcaccccg ccgacatccc
     7801 cgactacttc aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga tgaactacga
     7861 ggacggcggc gtggtgaccg tgacccagga ctcctccctg caggacggcg agttcatcta
     7921 caaggtgaag ctgcgcggca ccaacttccc ctccgacggc cccgtgatgc agaagaagac
     7981 catgggctgg gaggcctcct ccgagcggat gtaccccgag gacggtgccc tgaagggcaa
     8041 gatcaagatg aggctgaagc tgaaggacgg cggccactac acctccgagg tcaagaccac
     8101 ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac atcgtcgaca tcaagttgga
     8161 catcacctcc cacaacgagg actacaccat cgtggaacag tacgaacgcg ccgagggccg
     8221 ccactccacc ggcggcatgg acgagctgta caagagtgca cgcgtgagta agctgaaccc
     8281 tcctgatgag agtggccccg gctgcatgag ctgcaagtgt gtgctctcct gagcggccgc
     8341 taatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc
     8401 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg
     8461 tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt
     8521 gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac
     8581 tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc
     8641 tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct
     8701 gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct
     8761 cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct
     8821 caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct
     8881 tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc atcgataccg
     8941 tcgagactgc tgcaccagat cataatcagc cataccacat ttgtagaggt tttacttgct
     9001 ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt
     9061 gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     9121 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     9181 tcttaaggcg tgtttaaaca tataacttcg tataggatac tttatacgaa gttatcgcgt
     9241 acggccacca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc
     9301 gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat
     9361 gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc
     9421 tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg ctaccccgac
     9481 cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc
     9541 accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc
     9601 gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc
     9661 ctggggcaca agctggagta caactacaac agccacaacg tctatatcat ggccgacaag
     9721 cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg
     9781 cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc
     9841 gacaaccact acctgagcac ccagtccgcc ctgagcaaag accccaacga gaagcgcgat
     9901 cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg
     9961 tacaagagtg cacgcgtgag taagctgaac cctcctgatg agagtggccc cggctgcatg
    10021 agctgcaagt gtgtgctctc ctgagcggcc gctaatcaac ctctggatta caaaatttgt
    10081 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct
    10141 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat
    10201 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg
    10261 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag
    10321 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc
    10381 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg
    10441 tcggggaaat catcgtcctt tccttggctg ctcgcctgtg ttgccacctg gattctgcgc
    10501 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc
    10561 ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc
    10621 tccctttggg ccgcctcccc gcatcgatac cgtcgagact gctgcaccag atcataatca
    10681 gccataccac atttgtagag gttttacttg ctttaaaaaa cctcccacac ctccccctga
    10741 acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca gcttataatg
    10801 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
    10861 ctagttgtgg tttgtccaaa ctcatcaatg tatcttaagg cgtaggcgtg aagctcttca
    10921 tataacttcg tatagtatac cttatacgaa gttatcaagt actgccacca tggtgagcga
    10981 gctgattaag gagaacatgc acatgaagct gtacatggag ggcaccgtga acaaccacca
    11041 cttcaagtgc acatccgagg gcgaaggcaa gccctacgag ggcacccaga ccatgagaat
    11101 caaggcggtc gagggcggcc ctctcccctt cgccttcgac atcctggcta ccagcttcat
    11161 gtacggcagc aaaaccttca tcaaccacac ccagggcatc cccgacttct ttaagcagtc
    11221 cttccccgag ggcttcacat gggagagagt caccacatac gaagacgggg gcgtgctgac
    11281 cgctacccag gacaccagcc tccaggacgg ctgcctcatc tacaacgtca agatcagagg
    11341 ggtgaacttc ccatccaacg gccctgtgat gcagaagaaa acactcggct gggaggcctc
    11401 caccgagacc ctgtaccccg ctgacggcgg cctggaaggc agagccgaca tggccctgaa
    11461 gctcgtgggc gggggccacc tgatctgcaa cttgaagacc acatacagat ccaagaaacc
    11521 cgctaagaac ctcaagatgc ccggcgtcta ctatgtggac agaagactgg aaagaatcaa
    11581 ggaggccgac aaagagacct acgtcgagca gcacgaggtg gctgtggcca gatactgcga
    11641 cctccctagc aaactggggc acagaagtgc acgcgtgagt aagctgaacc ctcctgatga
    11701 gagtggcccc ggctgcatga gctgcaagtg tgtgctctcc tgagcggccg ctaatcaacc
    11761 tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac
    11821 gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt
    11881 cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt
    11941 tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg
    12001 cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac
    12061 ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac
    12121 tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt
    12181 tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc
    12241 ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg
    12301 ccctcagacg agtcggatct ccctttgggc cgcctccccg catcgatacc gtcgagactg
    12361 ctgcaccaga tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac
    12421 ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg
    12481 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
    12541 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttaaggc
    12601 gtaggcgtac tagctcgagg tccttcctct gcagaggtct tgcttctccc ggtcagctga
    12661 ctccctcccc aagtccttca aatatctcag aacatgggga gaaacgggga ccttgtccct
    12721 cctaaggaac cccagtgctg catgccatca tcccccccac cctcgccccc acccccgcca
    12781 cttctccctc catgcatacc actagctgtc attttgtact ctgtatttat tctagggctg
    12841 cttctgatta tttagtttgt tctttccctg gagacctgtt agaacataag ggcgtatggt
    12901 gggtagggga ggcaggatat cagtcccctg gggcgagttc ctccctgcca accaagccag
    12961 atgcctgaaa gagatatgga tgagggaagt tggactgtgc ctgtacctgg tacagtcata
    13021 ctctgttgaa agaatcatcg gggagggggg ggggctcaag aggggagagc tctctgagcc
    13081 tttgtggacc atccaatgag gatgagggct tagattctac caggtcattc tcagccacca
    13141 cacacaagcg ctctgccatc actgaagaag ccccctaggg ctcttgggcc agggcacact
    13201 cagtaaagat gcaggttcag tcagggaatg atggggaaag gggtaggagg tgggggaggg
    13261 atcaccccct cctctaaaac acgagcctgc tgtctccaaa ggcctctgcc tgtagtgagg
    13321 gtggcagaag aagacaagga gccagaactc tgactccagg atctaagtcc gtgcaggaag
    13381 gggatcctag aaccatctgg ttggacccag cttaccaagg gagagccttt attccttctt
    13441 tcccttgccc ctctgtgcca gcccctcttg ctgtccctga tcccccagac agcgagagtc
    13501 ttgcaacctg cctcttccaa gacctcctaa tctcaggggc aggcggtgga gtgagatccg
    13561 gcgtgcacac tttttggaag atagctttcc caaggatcct ctcccccact ggcagctctg
    13621 cctgtcccat caccatgtat aataccacca ctgctacagc atctcaccga ggaaagaaaa
    13681 ctgcacaata aaaccaagcc tctggagtgt gtcctggtgt ctgtctcttc tgtgtcctgg
    13741 cgtctgtctc ttctgtgttc ttccaaggtc agaaacaaaa accacacact tcaacctgga
    13801 tggctcggct gagcacttct gtgtgcagaa ggtccaacca gactctgggg taccccggcc
    13861 ctccctattc ccttgcctcc tgtctcccgc tttttatagc tccctatgct gggcttctct
    13921 ggagagtgaa atctttgccc aaatcaatgc gcattctctc tgctgagtca tctggcgaca
    13981 gcagttgagt tcacccgcca acacatgggc ccagctatgt agccgaaccc tggctctgga
    14041 agtgccaggg actttgtgca taagtatgta ccatgccctt ttttcacagt cctagctctg
    14101 cagaagtgca gcctgaaggc ctgtctgctg agaggacatg ccctggagcc ctgaaacagg
    14161 cacagtggga ggaggaacgg aggatgacag gcatcaggcc ctcagtccaa aagcaaccac
    14221 ttgagaatgg gctggagtac gaaacatggg gtcccgtccc tggatccctc ctcaaagagt
    14281 aataagtaaa atataaacag gtaccccagg ccgttctggg tttgggttgt aatgggatcc
    14341 atttgcagag aactattgag acagcccagc cgtactgtga caggcaatgt gggggaggag
    14401 gttgaatcac ttggtattta gcatgaatag aataattccc tgaacatttt tcttaaacat
    14461 ccatatctaa attaccacca ctcgctccca gtcttcctgc ctttgcgcca gcctcctgtc
    14521 tggccatgcc tgaagaaggc tggagaagcc acccacctca ggccatgaca ctgccagcca
    14581 cttggcaggt gcagccaaac ctgagctgtc ccagaaaggg acattctcaa gacccaggca
    14641 ccctgatcag cactgacttg gagctacaag tgtcatgcca gaaaagtctc taagaaaacc
    14701 ttttcaggga aaagggggtg actcaacacc gggcaagttt gggaagcccc acccttcgag
    14761 tgatggaaga gcagatagga agcctcagaa gagagacacc ggcacccagg taacgttcct
    14821 catgtggtct ctgtcacact aggtgctctt ccctggacat ctccgtgacc acactctcag
    14881 ttcttaggga gatgcgggtg ctctctgagg ctatctcaga gttgcagatt ctgaggccta
    14941 gagtgactac agtcagccta ggaagccaca gaggactgtg gaccaggagg gcagaagagg
    15001 agaagggaag aaaaaccatc agataggact tgcaatgaaa ctaacccaag acaatcataa
    15061 tgcagacagg aatgttaaag gcgttcagca gctggccatg acacccatct gtccctctgg
    15121 ccaagtcagc aagcctggaa gacctgggac tcctgcccat atgtcctaag ctccccaccc
    15181 acccactcgt tcactgtcct tattctctct ctaccttcag ccacttagtt tcctacctta
    15241 agtcctagaa ttgatcctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac
    15301 agttgcgcag cctgaatggc gaatggcgcc tgatgcggta ttttctcctt acgcatctgt
    15361 gcggtatttc acaccgcata tggtgcactc tcagtacaat ctgctctgat gccgcatagt
    15421 taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc
    15481 cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt
    15541 caccgtcatc accgaaacgc gcga
//
