LOCUS       sequence-74666-e       13275 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13275)
  AUTHORS   Cai D, Cohen KB, Luo T, Lichtman JW, Sanes JR
  TITLE     Improved tools for the Brainbow toolbox.
  JOURNAL   Nat Methods. 2013 May 5;10(6):540-7. doi: 10.1038/nmeth.2450. Epub 
            2013 May 5.
  PUBMED    23817127
REFERENCE   2  (bases 1 to 13275)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2450"; journalName: "Nat Methods"; date: "2013-05-5- 
            5"; volume: "10"; issue: "6"; pages: "540-7"
COMMENT     Sequence Label: pThy1-Flpbow1
FEATURES             Location/Qualifiers
     source          1..13275
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(10..28)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        96..200
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(201..269,270..1061)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(419..438)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1232..1820
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1721..1740
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1974..1991
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6281..6305
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    6312..6345
                     /label=FRT3
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision with other FRT3 sites
                     (Bosch et al., 2015)"
     protein_bind    6354..6387
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     protein_bind    6396..6429
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     regulatory      6434..6443
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6440..7870
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTA
                     KLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     LVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     QALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRH
                     HLFLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPY
                     EGTQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVM
                     NFEDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGV
                     LKGEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYE
                     RSEGRHHLFLYGMDELYK"
     polyA_signal    7992..8113
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    8128..8161
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     CDS             8175..8879
                     /codon_start=1
                     /product="Phialidium yellow fluorescent proteim"
                     /label=PhiYFP
                     /note="mammalian codon-optimized"
                     /translation="MSSGALLFHGKIPYVVEMEGNVDGHTFSIRGKGYGDASVGKVDAQ
                     FICTTGDVPVPWSTLVTTLTYGAQCFAKYGPELKDFYKSCMPDGYVQERTITFEGDGNF
                     KTRAEVTFENGSVYNRVKLNGQGFKKDGHVLGKNLEFNFTPHCLYIWGDQANHGLKSAF
                     KICHEITGSKGDFIVADHTQMNTPIGGGPVHVPEYHHMSYHVKLSKDVTDHRDNMSLKE
                     TVRAVDCRKTYL"
     polyA_signal    9062..9183
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    9211..9244
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     regulatory      9252..9261
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(9258..9260,9261..9263,9264..9977)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(9303..9324)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(9564..9583)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     9911..9932
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    10099..10220
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(10274..10298)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(13106..13125)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     13225..13247
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
      181 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
      241 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
      301 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
      361 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
      421 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
      481 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
      541 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
      601 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
      661 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
      721 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
      781 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
      841 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
      901 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
      961 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     1021 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     1081 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     1141 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     1201 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     1261 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     1321 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc
     1381 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     1441 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     1501 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     1561 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     1621 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     1681 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     1741 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     1801 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1861 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1921 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1981 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     2041 cgattcatta atgcaggatc gcggccgcga tccccgggcg agctcgaatt agttacgcta
     2101 gggataacag ggtaatatag aaaattcaga gaccgggaac caaactagcc tttaaaaaac
     2161 ataagtacag gagccagcaa gatggctcag tgggtaaagg tgcctaccag caagcctgac
     2221 agcctgagtt cagtccccac gaactacgtg gtaggagagg accaaccaac tctggaaatc
     2281 tgttctgcaa acacatgctc acacacacac acacaaatag tataaacaat tttaaatttc
     2341 atttaaaaat aatttgtaaa caaaatcatt agcacaggtt ttagaaagag cctcttggtg
     2401 acatcaagtt gatgctgtag atggggtatc attcctgagg acccaaaacc gggtctcagc
     2461 ctttccccat tctgagagtt ctctcttttc tcagccacta gctgaagagt agagtggctc
     2521 agcactgggc tcttgagttc ccaagtccta caactggtca gcctgactac taaccagcca
     2581 tgaagaaaca aggagtggat gggctgagtc tgctgggatg ggagtggagt tagtaagtgg
     2641 ccatggatgt aatgacccca gcaatgctgg ctagaaggca tgcctccttt ccttgtctgg
     2701 agacggaacg ggatcatctt gtactcacag aagggagaac attctagctg gttgggccaa
     2761 aatgtgcaag ttcacctgga ggtggtggtg catgctttta actccagtac tcaggaggca
     2821 gggccaggtg gatctctgtg agttcaagac cagcctgcac tatggagaga gttttgggac
     2881 agccagagtt acacagaaaa atcctggtgg aaaatctgaa agaaagagag aaagaaagaa
     2941 agaaagaaag aaagaaagaa agaaagaaag aaagaaagaa agaaagaaag aaaggaagaa
     3001 agaaagaaag agtggcaggc aggcaggcag gaggaaggaa ggaaggaagg aaggaaggaa
     3061 ggaaggaagg aaggaaggaa ggaaggaagg aaggaaaata ggtgcgactt caagatccgg
     3121 agttacaagc agaatgcact gtttccctaa cagggccaag tgttttgagt aactgaaggt
     3181 gggcatgatg cctgggaagc agaaacaagc caggcagatg caccccttgc cttgcttccg
     3241 aagggctgca gtagcatgga aaacatggaa aacaaccaat ccattccctt tgctgatata
     3301 acaggctcca aagccaaaac ctgtcactgg aggctcaaga gcagatctcc agccaagagg
     3361 caaaggaatg ggggaagctg gagggcctcc ctctggttat ccaggcttct gaaggttcaa
     3421 gcaaagaaag ggttacaacc ttaaaaggag agcgtcccgg ggtatgggta gaagactgct
     3481 ccaccccgac ccccagggtc cctaaccgtc ttttccctgg gcgagtcagc ccaatcacag
     3541 gactgagagt gcctctttag tagcagcaag ccacttcgga cacccaaatg gaacacctcc
     3601 agtcagccct cgccgaccac cccaccccct ccatcctttt ccctcagcct ccgattggct
     3661 gaatctagag tccctccctg ctcccccctc tctccccacc cctggtaaaa ctgcgggctt
     3721 caggctgggt gcagcaactg gaggcgttgg cgaccaggag gaggctgcag ctaggggagt
     3781 ccaggtgaga gcaggccgac gggagggacc cgcacatgca aggaccgccg cagggcgagg
     3841 atgcaagcct tccccagcta cagttttggg aaaggatacc agggcgctcc tatatggggg
     3901 cgcgggaacc tggggaaaga aggtgctccc aggtcgaggt gggagaggaa ggcagtgcgg
     3961 ggtcacgggc tttctccctg ctaacggacg ctttcgaaga gtgggtgccg gagagaacca
     4021 tgaggaagga catcaaggac agcctttggt ccccaagctc aaatcgcttt agtggtgcga
     4081 atagagggag gaggtgggtg gcaaactgga gggagtcccc agcggtgacc tcgtggctgg
     4141 ctggtgcggg gcaccaggta agaaacgcaa tgttgcggga ggggactggg tggcaggcgc
     4201 gggggagggg aaagctagaa aggatgcgag ggagcggagg ggggagggag cgggggaatc
     4261 tcaactggta gaggaaagtt aaaatgagga aatagcatca gggtggggtt agccaagccg
     4321 ggcctcaggg aaagggcgca aagtttgtct gggtgtgggc ttaggtgggc tgggtatgag
     4381 attcggggcg ccgaaaacac tgctgcgcct ctgccaaatc acgctacccc tgtatctagt
     4441 tctgctaggc ttctccagcc ccagccccaa ttcttttctc agtgtcccct tccctcccct
     4501 gaatctcaag cccacactcc ctcctccata acccactgtt atcaaatcta agtcatttgc
     4561 cacccaacaa ccatcaggag gcggaagcag acgggaggag tttgagatca acttgggcta
     4621 catcacgagt tccaagctca ccaaggcttc ttaaggagac cttgtctcta aaattaatta
     4681 attaattaat taatagtccc ctttctctgc cacagaacct tgggatctgg ctcctggtcg
     4741 cagctccccc caccccaggc tgacattcac tgccatagcc catccggaaa tcctagtcta
     4801 ttccccatgg atcttgaact gcagagagaa tggcagagtg gcccgccctg tgcaaaggat
     4861 gttcctagcc taggtggagc tcgcgaactc ggagactgtg cctctcttgg gcaaggacag
     4921 gctagacagc ctgccggtgt tgagctaggg cactgtgggg aaggcagaga acctgtgcag
     4981 ggcacgcaat gaacacagga ccagaaaact gcagccctag gaacactcaa gagctggcca
     5041 tttgcaagca tctctggcct ccgtgcttct cactcatgtc ccatgtctta tacaggcctc
     5101 tgtggcacct cgcttgcctg atctcatccc tagccgttaa gctttctgca tgacttatca
     5161 cttggggcat aatgctggat acctaccatt ttcttagacc ccatcaaaat cctatttgag
     5221 tgtacggttc ggagaactcc ttatttatcc ggtaaatgtc ttttactctg ctctcaggga
     5281 gctgaggcag gacattcctg agatacattg ggagaggaat acagtttcaa taaaataata
     5341 ggttgggtgg aggtacatgc ctataatgcc accactcagg aaatggtggc agcttcgtga
     5401 gtttgaggcc aacccaagaa acatagtgaa accctgtcag taaataagta agcaagtatt
     5461 tgagtatcta ctatatgcta gggctgacct ggacattagg ggtcatcttc tgaacaaact
     5521 agtgcttgag ggaggtattt ggggtttttg tttgtttaat ggatctgaat gagttccaga
     5581 gactggctac acagcgatat gactgagctt aacaccccta aagcatacag tcagaccaat
     5641 tagacaataa aaggtatgta tagcttacca aataaaaaaa ttgtattttc aagagagtgt
     5701 ctgtctgtgt agccctggct gttcttgaac tcactctgta gaccaggctg gcctggaaat
     5761 ccatctgcct ctgcctctct gcctctctgc ctctctgcct ctctctctgc ctctctctgc
     5821 ctctctctgc ccctctctgc ccctctctgc ccctctctgc cgccctctgc cttttgccct
     5881 ctgccctctg ttctctggcc tctgccctct ccctgccttc tccctctgcc tctgcctctt
     5941 gagtgctgga atcaaaggtg tgagctctat aggtcttaag ttccagaaga aacgtaatga
     6001 agtcacccag caggaggtgc tcagggacag caagacacac acacccagga cactaggctc
     6061 ccacttcctt ggctttctct gagtggcaaa ggaccttagg cagtgtcact ccctaagaga
     6121 aggggataaa gagaggggct gaggtattca tcatgtgctc cgtggatctc aagccctcaa
     6181 ggtaaatggg gacccacctg tcctaccagc tggctgacct gtagctttcc ccaccacaga
     6241 atccaagtcg gaactcttgg cacctagagg atctcgaatc acaagtttgt acaaaaaagc
     6301 aggctcctag ggaagttcct attcttcaaa tagtatagga acttccggcg cgcgaagttc
     6361 ctattctcta gaaggtatag gaacttcagc gctaggaagt tcctattctc taaaaagtat
     6421 aggaacttct atcgccacca tggtgagcaa gggcgaggag gtcatcaaag agttcatgcg
     6481 cttcaaggtg cgcatggagg gctccatgaa cggccacgag ttcgagatcg agggcgaggg
     6541 cgagggccgc ccctacgagg gcacccagac cgccaagctg aaggtgacca agggcggccc
     6601 cctgcccttc gcctgggaca tcctgtcccc ccagttcatg tacggctcca aggcgtacgt
     6661 gaagcacccc gccgacatcc ccgattacaa gaagctgtcc ttccccgagg gcttcaagtg
     6721 ggagcgcgtg atgaacttcg aggacggcgg tctggtgacc gtgacccagg actcctccct
     6781 gcaggacggc acgctgatct acaaggtgaa gatgcgcggc accaacttcc cccccgacgg
     6841 ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc accgagcgcc tgtacccccg
     6901 cgacggcgtg ctgaagggcg agatccacca ggccctgaag ctgaaggacg gcggccacta
     6961 cctggtggag ttcaagacca tctacatggc caagaagccc gtgcaactgc ccggctacta
     7021 ctacgtggac accaagctgg acatcacctc ccacaacgag gactacacca tcgtggaaca
     7081 gtacgagcgc tccgagggcc gccaccacct gttcctgggg catggcaccg gcagcaccgg
     7141 cagcggcagc tccggcaccg cctcctccga ggacaacaac atggccgtca tcaaagagtt
     7201 catgcgcttc aaggtgcgca tggagggctc catgaacggc cacgagttcg agatcgaggg
     7261 cgagggcgag ggccgcccct acgagggcac ccagaccgcc aagctgaagg tgaccaaggg
     7321 cggccccctg cccttcgcct gggacatcct gtccccccag ttcatgtacg gctccaaggc
     7381 gtacgtgaag caccccgccg acatccccga ttacaagaag ctgtccttcc ccgagggctt
     7441 caagtgggag cgcgtgatga acttcgagga cggcggtctg gtgaccgtga cccaggactc
     7501 ctccctgcag gacggcacgc tgatctacaa ggtgaagatg cgcggcacca acttcccccc
     7561 cgacggcccc gtaatgcaga agaagaccat gggctgggag gcctccaccg agcgcctgta
     7621 cccccgcgac ggcgtgctga agggcgagat ccaccaggcc ctgaagctga aggacggcgg
     7681 ccactacctg gtggagttca agaccatcta catggccaag aagcccgtgc aactgcccgg
     7741 ctactactac gtggacacca agctggacat cacctcccac aacgaggact acaccatcgt
     7801 ggaacagtac gagcgctccg agggccgcca ccacctgttc ctgtacggca tggacgagct
     7861 gtacaagtaa gcggccgcga ctctagatca taatcagcca taccacattt gtagaggttt
     7921 tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa
     7981 ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     8041 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     8101 tcaatgtatc ttaaggcgtg tttaaacgaa gttcctattc tctagaaggt ataggaactt
     8161 cccgtacggc caccatgagc agcggcgccc tgctgttcca cggcaagatc ccctacgtgg
     8221 tggagatgga gggcaatgtg gatggccaca ccttcagcat ccgcggcaag ggctacggcg
     8281 atgccagcgt gggcaaggtg gatgcccagt tcatctgcac caccggcgat gtgcccgtgc
     8341 cctggagcac cctggtgacc accctgacct acggcgccca gtgcttcgcc aagtacggcc
     8401 ccgagctgaa ggatttctac aagagctgca tgcccgatgg ctacgtgcag gagcgcacca
     8461 tcaccttcga gggtgatggc aatttcaaga cccgcgccga ggtgaccttc gagaatggca
     8521 gcgtgtacaa tcgcgtgaag ctgaatggcc agggcttcaa gaaggatggc cacgtgctgg
     8581 gcaagaatct ggagttcaat ttcacccccc actgcctgta catctggggc gatcaggcca
     8641 atcacggcct gaagagcgcc ttcaagatct gccacgagat caccggcagc aagggcgatt
     8701 tcatcgtggc cgatcacacc cagatgaata cccccatcgg cggcggcccc gtgcacgtgc
     8761 ccgagtacca ccacatgagc taccacgtga agctgagcaa ggatgtgacc gatcaccgcg
     8821 ataatatgag cctgaaggag accgtgcgcg ccgtggattg ccgcaagacc tacctgtgaa
     8881 gcttaattag ctgagcttgg actcctgttg atagatccag taatgacctc agaactccat
     8941 ctggccgcga ctctagatca taatcagcca taccacattt gtagaggttt tacttgcttt
     9001 aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt
     9061 taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     9121 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     9181 ttaaggcgtg aagagcttca taatcttcag gaagttccta ttctctaaaa agtataggaa
     9241 cttccagtac tgccaccatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca
     9301 tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg
     9361 agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc
     9421 ccgtgccctg gcccaccctc gtgaccaccc tgacctgggg cgtgcagtgc ttcgcccgct
     9481 accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc
     9541 aggagcgcac catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt
     9601 tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg
     9661 gcaacatcct ggggcacaag ctggagtaca acgccatcag cgacaacgtc tatatcaccg
     9721 ccgacaagca gaagaacggc atcaaggcca acttcaagat ccgccacaac atcgaggacg
     9781 gcagcgtgca gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc
     9841 tgctgcccga caaccactac ctgagcaccc agtccaagct gagcaaagac cccaacgaga
     9901 agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg
     9961 acgagctgta caagtaagcg gccgcgactc tagatcataa tcagccatac cacatttgta
    10021 gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg
    10081 aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat
    10141 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
    10201 aaactcatca atgtatctta aggcgtacta gttgacgtaa ctataacggt cctaaggtag
    10261 cgaatgcatc tagacccagc tttcttgtac aaagtggtga ttcgaggtcc ttcctctgca
    10321 gaggtcttgc ttctcccggt cagctgactc cctccccaag tccttcaaat atctcagaac
    10381 atggggagaa acggggacct tgtccctcct aaggaacccc agtgctgcat gccatcatcc
    10441 cccccaccct cgcccccacc cccgccactt ctccctccat gcataccact agctgtcatt
    10501 ttgtactctg tatttattct agggctgctt ctgattattt agtttgttct ttccctggag
    10561 acctgttaga acataagggc gtatggtggg taggggaggc aggatatcag tcccctgggg
    10621 cgagttcctc cctgccaacc aagccagatg cctgaaagag atatggatga gggaagttgg
    10681 actgtgcctg tacctggtac agtcatactc tgttgaaaga atcatcgggg aggggggggg
    10741 gctcaagagg ggagagctct ctgagccttt gtggaccatc caatgaggat gagggcttag
    10801 attctaccag gtcattctca gccaccacac acaagcgctc tgccatcact gaagaagccc
    10861 cctagggctc ttgggccagg gcacactcag taaagatgca ggttcagtca gggaatgatg
    10921 gggaaagggg taggaggtgg gggagggatc accccctcct ctaaaacacg agcctgctgt
    10981 ctccaaaggc ctctgcctgt agtgagggtg gcagaagaag acaaggagcc agaactctga
    11041 ctccaggatc taagtccgtg caggaagggg atcctagaac catctggttg gacccagctt
    11101 accaagggag agcctttatt ccttctttcc cttgcccctc tgtgccagcc cctcttgctg
    11161 tccctgatcc cccagacagc gagagtcttg caacctgcct cttccaagac ctcctaatct
    11221 caggggcagg cggtggagtg agatccggcg tgcacacttt ttggaagata gctttcccaa
    11281 ggatcctctc ccccactggc agctctgcct gtcccatcac catgtataat accaccactg
    11341 ctacagcatc tcaccgagga aagaaaactg cacaataaaa ccaagcctct ggagtgtgtc
    11401 ctggtgtctg tctcttctgt gtcctggcgt ctgtctcttc tgtgttcttc caaggtcaga
    11461 aacaaaaacc acacacttca acctggatgg ctcggctgag cacttctgtg tgcagaaggt
    11521 ccaaccagac tctggggtac cccggccctc cctattccct tgcctcctgt ctcccgcttt
    11581 ttatagctcc ctatgctggg cttctctgga gagtgaaatc tttgcccaaa tcaatgcgca
    11641 ttctctctgc tgagtcatct ggcgacagca gttgagttca cccgccaaca catgggccca
    11701 gctatgtagc cgaaccctgg ctctggaagt gccagggact ttgtgcataa gtatgtacca
    11761 tgcccttttt tcacagtcct agctctgcag aagtgcagcc tgaaggcctg tctgctgaga
    11821 ggacatgccc tggagccctg aaacaggcac agtgggagga ggaacggagg atgacaggca
    11881 tcaggccctc agtccaaaag caaccacttg agaatgggct ggagtacgaa acatggggtc
    11941 ccgtccctgg atccctcctc aaagagtaat aagtaaaata taaacaggta ccccaggccg
    12001 ttctgggttt gggttgtaat gggatccatt tgcagagaac tattgagaca gcccagccgt
    12061 actgtgacag gcaatgtggg ggaggaggtt gaatcacttg gtatttagca tgaatagaat
    12121 aattccctga acatttttct taaacatcca tatctaaatt accaccactc gctcccagtc
    12181 ttcctgcctt tgcgccagcc tcctgtctgg ccatgcctga agaaggctgg agaagccacc
    12241 cacctcaggc catgacactg ccagccactt ggcaggtgca gccaaacctg agctgtccca
    12301 gaaagggaca ttctcaagac ccaggcaccc tgatcagcac tgacttggag ctacaagtgt
    12361 catgccagaa aagtctctaa gaaaaccttt tcagggaaaa gggggtgact caacaccggg
    12421 caagtttggg aagccccacc cttcgagtga tggaagagca gataggaagc ctcagaagag
    12481 agacaccggc acccaggtaa cgttcctcat gtggtctctg tcacacggtc gactctctta
    12541 aggtagctcg accgtgctct tccctggaca tctccgtgac cacactctca gttcttaggg
    12601 agatgcgggt gctctctgag gctatctcag agttgcagat tctgaggcct agagtgacta
    12661 cagtcagcct aggaagccac agaggactgt ggaccaggag ggcagaagag gagaagggaa
    12721 gaaaaaccat cagataggac ttgcaatgaa actaacccaa gacaatcata atgcagacag
    12781 gaatgttaaa ggcgttcagc agctggccat gacacccatc tgtccctctg gccaagtcag
    12841 caagcctgga agacctggga ctcctgccca tatgtcctaa gctccccacc cacccactcg
    12901 ttcactgtcc ttattctctc tctaccttca gccacttagt ttcctacctt aagtcctaga
    12961 attgatcctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca
    13021 gcctgaatgg cgaatggcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt
    13081 cacaccgcat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagc
    13141 cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg
    13201 cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat
    13261 caccgaaacg cgcga
//
