LOCUS       sequence-74667-e         993 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  gene deletion in S. pombe.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 993)
  AUTHORS   Gadaleta MC, Iwasaki O, Noguchi C, Noma K, Noguchi E
  TITLE     New vectors for epitope tagging and gene disruption in 
            Schizosaccharomyces pombe.
  JOURNAL   Biotechniques. 2013 Nov;55(5):257-63. doi: 10.2144/000114100.
  PUBMED    24215641
REFERENCE   2  (bases 1 to 993)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Biotechniques"; date: "2013-11"; volume: "55"; issue: "5"; pages: 
            "257-63"
COMMENT     Sequence Label: pFA6a-ura4MX6
FEATURES             Location/Qualifiers
     source          1..993
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        59..77
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     59..76
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 tattgtcgtt agaacgcggc tacaattaat acataacctt atgtatcata cacatacgat
       61 ttaggtgaca ctatagaacg cggccgccag ctgaagcttc gtacgctgca ggtcgacgga
      121 tccccgggtt aattaaggcg cgccagatct aagcttgtga tattgacgaa actttttgac
      181 atctaattta ttctgttcca acaccaatgt ttataaccaa gttttatctt gtttgtctac
      241 atggtatttt acattcatct acatacatct ttcattggct ttgtacatag ttatcattac
      301 aagtctaaaa aaattcactc ttttcttatt caatgtcaat ccaagagaaa agattgtggt
      361 aatgttgtag gagcatgttt aataaattac tatagcaaat tactttttat tcccaaggtg
      421 tttatctata atagttaata ttttagtcgc tacataaaat tttaccaaag agtacttgta
      481 tactaattct aaatgccttc tgacataaaa cgcctaggaa aacaaacgca aacaaggcat
      541 cgactttttc aataaccaac caaaaaaatt ttacattagt ctttttttaa tgctgagaaa
      601 gtctttgctg atatgccttc caaccagctt ctctatatct cttggcttcg acaacaggat
      661 tacgaccagc tccatagact ccacgaccaa caatgatgat atcgctaccg cagtttacaa
      721 tcacttcttc aggagtacga tattgctgtc ccagcccgtc tcctttaaca tccaagccga
      781 taccagggga catagttatg tagtcgcttt gaaggttagg aaatcgacga ccagctataa
      841 agccaaagca aaaatcggta tgcttctcaa accattctaa ggttttctct gtgtaggaac
      901 cagtagccaa agagcctttg gaagacattt cagccaaaag caagagacca cgtcccaaag
      961 gtaaaccaac ttctttgagg ccttgtataa tac
//
