LOCUS       sequence-74668-e         863 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  genomic 12Pk epitope tagging in S. pombe.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 863)
  AUTHORS   Gadaleta MC, Iwasaki O, Noguchi C, Noma K, Noguchi E
  TITLE     New vectors for epitope tagging and gene disruption in 
            Schizosaccharomyces pombe.
  JOURNAL   Biotechniques. 2013 Nov;55(5):257-63. doi: 10.2144/000114100.
  PUBMED    24215641
REFERENCE   2  (bases 1 to 863)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Biotechniques"; date: "2013-11"; volume: "55"; issue: "5"; pages: 
            "257-63"
COMMENT     Sequence Label: pFA6a-12Pk-LEU2MX6
FEATURES             Location/Qualifiers
     source          1..863
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(71..670)
                     /codon_start=1
                     /product="12 tandem copies of the Pk (V5) epitope"
                     /label=12Pk tag
                     /translation="GKPIPNPLLGLDSTSGGKPIPNPLLGLDSTLDGGGKPIPNPLLGL
                     DSTSGGKPIPNPLLGLDSTLDGGGKPIPNPLLGLDSTSGGKPIPNPLLGLDSTLDGGGK
                     PIPNPLLGLDSTSGGKPIPNPLLGLDSTLDGGGKPIPNPLLGLDSTSGGKPIPNPLLGL
                     DSTLDGGGKPIPNPLLGLDSTSGGKPIPNPLLGLDST"
     promoter        complement(743..761)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(744..761)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(842..861)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 acttatttaa taataaaaat cataaatcat aagaaattcg cttatttaga agtggcgcgc
       61 cctactcgag tgtagaatca agacccagca gagggttagg gataggctta cctccggatg
      121 tagaatcaag acccagcaga gggttaggga taggcttacc tccaccgtcg agtgtagaat
      181 caagacccag cagagggtta gggataggct tacctccgga tgtagaatca agacccagca
      241 gagggttagg gataggctta cctccaccgt cgagtgtaga atcaagaccc agcagagggt
      301 tagggatagg cttacctccg gatgtagaat caagacccag cagagggtta gggataggct
      361 tacctccacc gtcgagtgta gaatcaagac ccagcagagg gttagggata ggcttacctc
      421 cggatgtaga atcaagaccc agcagagggt tagggatagg cttacctcca ccgtcgagtg
      481 tagaatcaag acccagcaga gggttaggga taggcttacc tccggatgta gaatcaagac
      541 ccagcagagg gttagggata ggcttacctc caccgtcgag tgtagaatca agacccagca
      601 gagggttagg gataggctta cctccggatg tagaatcaag acccagcaga gggttaggga
      661 taggcttacc tccaccgtcg aggttaatta acccggggat ccgtcgacct gcagcgtacg
      721 aagcttcagc tggcggccgc gttctatagt gtcacctaaa tcgtatgtgt atgatacata
      781 aggttatgta ttaattgtag ccgcgttcta acgacaatat gtccatatgg tgcactctca
      841 gtacaatctg ctctgatgcc gca
//
