LOCUS       sequence-74669-e         762 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  genomic 12Pk epitope tagging in S. pombe.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 762)
  AUTHORS   Gadaleta MC, Iwasaki O, Noguchi C, Noma K, Noguchi E
  TITLE     New vectors for epitope tagging and gene disruption in 
            Schizosaccharomyces pombe.
  JOURNAL   Biotechniques. 2013 Nov;55(5):257-63. doi: 10.2144/000114100.
  PUBMED    24215641
REFERENCE   2  (bases 1 to 762)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Biotechniques"; date: "2013-11"; volume: "55"; issue: "5"; pages: 
            "257-63"
COMMENT     Sequence Label: pFA6a-12Pk-LEU2MX6
FEATURES             Location/Qualifiers
     source          1..762
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        57..75
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     57..74
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             148..747
                     /codon_start=1
                     /product="12 tandem copies of the Pk (V5) epitope"
                     /label=12Pk tag
                     /translation="GKPIPNPLLGLDSTSGGKPIPNPLLGLDSTLDGGGKPIPNPLLGL
                     DSTSGGKPIPNPLLGLDSTLDGGGKPIPNPLLGLDSTSGGKPIPNPLLGLDSTLDGGGK
                     PIPNPLLGLDSTSGGKPIPNPLLGLDSTLDGGGKPIPNPLLGLDSTSGGKPIPNPLLGL
                     DSTLDGGGKPIPNPLLGLDSTSGGKPIPNPLLGLDST"
ORIGIN
        1 ttgtcgttag aacgcggcta caattaatac ataaccttat gtatcataca catacgattt
       61 aggtgacact atagaacgcg gccgccagct gaagcttcgt acgctgcagg tcgacggatc
      121 cccgggttaa ttaacctcga cggtggaggt aagcctatcc ctaaccctct gctgggtctt
      181 gattctacat ccggaggtaa gcctatccct aaccctctgc tgggtcttga ttctacactc
      241 gacggtggag gtaagcctat ccctaaccct ctgctgggtc ttgattctac atccggaggt
      301 aagcctatcc ctaaccctct gctgggtctt gattctacac tcgacggtgg aggtaagcct
      361 atccctaacc ctctgctggg tcttgattct acatccggag gtaagcctat ccctaaccct
      421 ctgctgggtc ttgattctac actcgacggt ggaggtaagc ctatccctaa ccctctgctg
      481 ggtcttgatt ctacatccgg aggtaagcct atccctaacc ctctgctggg tcttgattct
      541 acactcgacg gtggaggtaa gcctatccct aaccctctgc tgggtcttga ttctacatcc
      601 ggaggtaagc ctatccctaa ccctctgctg ggtcttgatt ctacactcga cggtggaggt
      661 aagcctatcc ctaaccctct gctgggtctt gattctacat ccggaggtaa gcctatccct
      721 aaccctctgc tgggtcttga ttctacactc gagtagggcg cg
//
