LOCUS       sequence-74671-e         759 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses kinase deficient FLAG-tagged human Erk1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 759)
  TITLE     cobb lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 759)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pFLAG-CMV-hErk1(K71R)
FEATURES             Location/Qualifiers
     source          1..759
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             19..84
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
ORIGIN
        1 ccgtcagaat taaccatgga ctacaaagac catgacggtg attataaaga tcatgacatc
       61 gattacaagg atgacgatga caagcttgcg gccgcgaatt caatggcggc ggcggcggct
      121 caggggggcg ggggcgggga gccccgtaga accgaggggg tcggcccggg ggtcccgggg
      181 gaggtggaga tggtgaaggg gcagccgttc gacgtgggcc cgcgctacac gcagttgcag
      241 tacatcggcg agggcgcgta cggcatggtc agctcggcct atgaccacgt gcgcaagact
      301 cgcgtggcca tccggaagat cagccccttc gaacatcaga cctactgcca gcgcacgctc
      361 cgggagatcc agatcctgct gcgcttccgc catgagaatg tcatcggcat ccgagacatt
      421 ctgcgggcgt ccaccctgga agccatgaga gatgtctaca ttgtgcagga cctgatggag
      481 actgacctgt acaagttgct gaaaagccag cagctgagca atgaccatat ctgctacttc
      541 ctctaccaga tcctgcgggg cctcaagtac atccactccg ccaacgtgct ccaccgagat
      601 ctaaagccct ccaacctgct catcaacacc acctgcgacc ttaagatttg tgatttcggc
      661 ctggcccgga ttgccgatcc tgagcatgac cacaccggct tcctgacgga gtatgtggct
      721 acgcgctggt accgggcccc agagatcatg ctgaactcc
//
