LOCUS       sequence-74678-e         822 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  genomic 12Pk epitope tagging in S. pombe.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 822)
  AUTHORS   Gadaleta MC, Iwasaki O, Noguchi C, Noma K, Noguchi E
  TITLE     New vectors for epitope tagging and gene disruption in 
            Schizosaccharomyces pombe.
  JOURNAL   Biotechniques. 2013 Nov;55(5):257-63. doi: 10.2144/000114100.
  PUBMED    24215641
REFERENCE   2  (bases 1 to 822)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Biotechniques"; date: "2013-11"; volume: "55"; issue: "5"; pages: 
            "257-63"
COMMENT     Sequence Label: pFA6a-12Pk-hphMX6
FEATURES             Location/Qualifiers
     source          1..822
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(69..668)
                     /codon_start=1
                     /product="12 tandem copies of the Pk (V5) epitope"
                     /label=12Pk tag
                     /translation="GKPIPNPLLGLDSTSGGKPIPNPLLGLDSTLDGGGKPIPNPLLGL
                     DSTSGGKPIPNPLLGLDSTLDGGGKPIPNPLLGLDSTSGGKPIPNPLLGLDSTLDGGGK
                     PIPNPLLGLDSTSGGKPIPNPLLGLDSTLDGGGKPIPNPLLGLDSTSGGKPIPNPLLGL
                     DSTLDGGGKPIPNPLLGLDSTSGGKPIPNPLLGLDST"
     promoter        complement(741..759)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(742..759)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 ttatttaata ataaaaatca taaatcataa gaaattcgct tatttagaag tggcgcgccc
       61 tactcgagtg tagaatcaag acccagcaga gggttaggga taggcttacc tccggatgta
      121 gaatcaagac ccagcagagg gttagggata ggcttacctc caccgtcgag tgtagaatca
      181 agacccagca gagggttagg gataggctta cctccggatg tagaatcaag acccagcaga
      241 gggttaggga taggcttacc tccaccgtcg agtgtagaat caagacccag cagagggtta
      301 gggataggct tacctccgga tgtagaatca agacccagca gagggttagg gataggctta
      361 cctccaccgt cgagtgtaga atcaagaccc agcagagggt tagggatagg cttacctccg
      421 gatgtagaat caagacccag cagagggtta gggataggct tacctccacc gtcgagtgta
      481 gaatcaagac ccagcagagg gttagggata ggcttacctc cggatgtaga atcaagaccc
      541 agcagagggt tagggatagg cttacctcca ccgtcgagtg tagaatcaag acccagcaga
      601 gggttaggga taggcttacc tccggatgta gaatcaagac ccagcagagg gttagggata
      661 ggcttacctc caccgtcgag gttaattaac ccggggatcc gtcgacctgc agcgtacgaa
      721 gcttcagctg gcggccgcgt tctatagtgt cacctaaatc gtatgtgtat gatacataag
      781 gttatgtatt aattgtagcc gcgttctaac gacaatatgt cc
//
