LOCUS       sequence-74688-e         995 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of AND gate in bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 995)
  AUTHORS   Stanton BC, Nielsen AA, Tamsir A, Clancy K, Peterson T, Voigt CA
  TITLE     Genomic mining of prokaryotic repressors for orthogonal logic gates.
  JOURNAL   Nat Chem Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411.
  PUBMED    24316737
REFERENCE   2  (bases 1 to 995)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411."
COMMENT     Sequence Label: pAND
FEATURES             Location/Qualifiers
     source          1..995
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             37..48
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     terminator      complement(130..157)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      complement(173..244)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     protein_bind    904..923
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     promoter        complement(join(929..935,936..951,952..957))
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
ORIGIN
        1 ggtgaacagc tcctcgccct tgctcaccat ctagtatttc tcctctttct ctagtagcta
       61 gcattatatt gaacgtccaa tcaattgtca attggtaacg aatccctctc acccgcgctc
      121 tcctataaac gcagaaaggc ccacccgaag gtgagccagt gtgactctag tagagagcgt
      181 tcaccgacaa acaacagata aaacgaaagg cccagtcttt cgactgagcc tttcgtttta
      241 tttgatgcct gggggtatgg gccctagtta acgctgtgta cccggacaaa caccattaat
      301 cagcaggaag gtaaattctt caatatcctg ttcaacggtc agctgttcgg tcagcaggcg
      361 ataccaacaa aaaccaaaaa tcatatccag cagcagttca cgattggtat ctttcggcag
      421 ttcaccattg ctaatggcat tttcaaccag ttttttcggc atctcacgac gacgttccat
      481 aaactgatct ttcagctggg tcagggttgc agggtccagc tgtgcttctg caataacaca
      541 acgaaatgct tcaccacaaa tggtttcacg ccaaactttc cacagattac gcagcagaaa
      601 atccagatcg gctttaaagc tacccagatc cggaaattta cgcacctgtt cgctttcatt
      661 ttcatacact tcggcaatca gtgctgcttt attggtccac caacgataaa tggtcggttt
      721 gcttgcaccg gcacgacgtg caacgctttc aatgctcaga ccgctataac cacattcttt
      781 caggatttca atggtgctgg tcagaattgc tttatgggta tgcggactac gcaggctacc
      841 aatgctgcta cggctcgggg tacgtgccat ctagtatttc tccctttcaa acatagtcca
      901 tagaattgtg agcgctcaca attccacaca ttatacgagc cgatgattaa ttgtcaacaa
      961 gcgtataaac gcagaaaggc ccacccgaag gtgag
//
