LOCUS       sequence-74689-e         998 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of AND gate in bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 998)
  AUTHORS   Stanton BC, Nielsen AA, Tamsir A, Clancy K, Peterson T, Voigt CA
  TITLE     Genomic mining of prokaryotic repressors for orthogonal logic gates.
  JOURNAL   Nat Chem Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411.
  PUBMED    24316737
REFERENCE   2  (bases 1 to 998)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411."
COMMENT     Sequence Label: pAND
FEATURES             Location/Qualifiers
     source          1..998
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      795..866
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      882..909
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     protein_bind    940..958
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    965..983
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
ORIGIN
        1 cgctcgacgt acggtggaat ctgattcgtt accaattgac atgatacgaa acgtaccgta
       61 tcgttaaggt ggtatggaag ctatacgtta ccaattgaca gctagctcag tcctacttta
      121 gtatatagac cgtgcgatcg gtctatagct acgacttgct catttgacag aggataacta
      181 ctagtgccga aactgggtat gcagagcatt cgtcgtcgtc agctgattga tgcaaccctg
      241 gaagcaatta atgaagttgg tatgcatgat gcaaccattg cacagattgc acgtcgtgcc
      301 ggtgttagca ccggtattat tagccattat ttccgcgata aaaacggtct gctggaagca
      361 accatgcgtg atattaccag ccagctgcgt gatgcagttc tgaatcgtct gcatgcactg
      421 ccgcagggta gcgcagaaca gcgtctgcag gcaattgttg gtggtaattt tgatgaaacc
      481 caggttagca gcgcagcaat gaaagcatgg ctggcatttt gggcaagcag catgcatcag
      541 ccgatgctgt atcgtctgca gcaggttagc agtcgtcgtc tgctgagcaa tctggttagc
      601 gaatttcgtc gtgaactgcc tcgtgaacag gcacaagagg caggttatgg tctggcagca
      661 ctgattgatg gtctgtggct gcgtgcagca ctgagcggta aaccgctgga taaaacccgt
      721 gcaaatagcc tgacccgtca ttttatcacc cagcatctgc cgaccgatta atcgtaaggc
      781 gagcccccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta
      841 tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc
      901 ctttctgcgt ttatagcttt actccaccgt tggctttttt ccctatcagt gatagagatt
      961 gacatcccta tcagtgatag agataatgag cacgccat
//
