LOCUS       sequence-74700-e         195 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  BIOFAB reporter plasmid for measuring M13_central_T_linker_rrnD_T1 
            termination efficiency.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 195)
  AUTHORS   Mutalik VK, Guimaraes JC, Cambray G, Lam C, Christoffersen MJ, Mai 
            QA, Tran AB, Paull M, Keasling JD, Arkin AP, Endy D
  TITLE     Precise and reliable gene expression via standard transcription and 
            translation initiation elements.
  JOURNAL   Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 
            2013 Mar 10.
  PUBMED    23474465
REFERENCE   2  (bases 1 to 195)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.2404"; journalName: "Nat Methods"; date: "2013-04"; 
            volume: "10"; issue: "4"; pages: "354-60"
COMMENT     Sequence Label: pFAB1902
FEATURES             Location/Qualifiers
     source          1..195
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      16..64
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
ORIGIN
        1 aaagcaagct gataaaccga tacaattaaa ggctcctttt ggagcctttt tttttggaga
       61 ttttcaacat gaaaaaatta ttatttgatg atcagatagc ggcggggaac tgccagacat
      121 caaataaaac aaaaggctca gtcggaagac tgggcctttt gttttatctg ttgtttgtcg
      181 gtgaacactc tcccg
//
