LOCUS       sequence-74704-e         824 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 824)
  AUTHORS   Osterwalder M, Galli A, Rosen B, Skarnes WC, Zeller R, Lopez-Rios J.
  TITLE     Dual RMCE for efficient re-engineering of mouse mutant alleles
  JOURNAL   Nat Methods. 2010 Oct 17
  PUBMED    20953177
REFERENCE   2  (bases 1 to 824)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2010 Oct 17"
COMMENT     Sequence Label: pDIRE
FEATURES             Location/Qualifiers
     source          1..824
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      24..33
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             36..56
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 ctccgggcct ttcgaccatc gccgccacca tggctcctaa gaagaagagg aaggtgatga
       61 gccagttcga catcctgtgc aagacccccc ccaaggtgct ggtgcggcag ttcgtggaga
      121 gattcgagag gcccagcggc gagaagatcg ccagctgtgc cgccgagctg acctacctgt
      181 gctggatgat cacccacaac ggcaccgcca tcaagagggc caccttcatg agctacaaca
      241 ccatcatcag caacagcctg agcttcgaca tcgtgaacaa gagcctgcag ttcaagtaca
      301 agacccagaa ggccaccatc ctggaggcca gcctgaagaa gctgatcccc gcctgggagt
      361 tcaccatcat cccttacaac ggccagaagc accagagcga catcaccgac atcgtgtcca
      421 gcctgcagct gcagttcgag agcagcgagg aggccgacaa gggcaacagc cacagcaaga
      481 agatgctgaa ggccctgctg tccgagggcg agagcatctg ggagatcacc gagaagatcc
      541 tgaacagctt cgagtacacc agcaggttca ccaagaccaa gaccctgtac cagttcctgt
      601 tcctggccac attcatcaac tgcggcaggt tcagcgacat caagaacgtg gaccccaaga
      661 gcttcaagct ggtgcagaac aagtacctgg gcgtgatcat tcagtgcctg gtgaccgaga
      721 ccaagacaag cgtgtccagg cacatctact ttttcagcgc cagaggcagg atcgaccccc
      781 tggtgtacct ggacgagttc ctgaggaaca gcgagcccgt gctg
//
