LOCUS       sequence-74705-e         841 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 841)
  AUTHORS   Osterwalder M, Galli A, Rosen B, Skarnes WC, Zeller R, Lopez-Rios J.
  TITLE     Dual RMCE for efficient re-engineering of mouse mutant alleles
  JOURNAL   Nat Methods. 2010 Oct 17
  PUBMED    20953177
REFERENCE   2  (bases 1 to 841)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2010 Oct 17"
COMMENT     Sequence Label: pDIRE
FEATURES             Location/Qualifiers
     source          1..841
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        8..26
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     54..70
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     55..71
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        96..595
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(131..154)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     528..547
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     regulatory      605..614
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             617..637
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 cagctcgaat taaccctcac taaagggaac aaaagctggt accgggcccc ccctcgaggt
       61 cgacggtatc gataagcttg atatcgaatt ctaccgggta ggggaggcgc ttttcccaag
      121 gcagtctgga gcatgcgctt tagcagcccc gctgggcact tggcgctaca caagtggcct
      181 ctggcctcgc acacattcca catccaccgg taggcgccaa ccggctccgt tctttggtgg
      241 ccccttcgcg ccaccttcta ctcctcccct agtcaggaag ttcccccccg ccccgcagct
      301 cgcgtcgtgc aggacgtgac aaatggaagt agcacgtctc actagtctcg tgcagatgga
      361 cagcaccgct gagcaatgga agcgggtagg cctttggggc agcggccaat agcagctttg
      421 ctccttcgct ttctgggctc agaggctggg aaggggtggg tccgggggcg ggctcagggg
      481 cgggctcagg ggcggggcgg gcgcccgaag gtcctccgga ggcccggcat tctgcacgct
      541 tcaaaagcgc acgtctgccg cgctgttctc ctcttcctca tctccgggcc tttcgaccat
      601 cgccgccacc atggctccta agaagaagag gaaggtgatg agccagttcg acatcctgtg
      661 caagaccccc cccaaggtgc tggtgcggca gttcgtggag agattcgaga ggcccagcgg
      721 cgagaagatc gccagctgtg ccgccgagct gacctacctg tgctggatga tcacccacaa
      781 cggcaccgcc atcaagaggg ccaccttcat gagctacaac accatcatca gcaacagcct
      841 g
//
