LOCUS       sequence-74708-e         867 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  donor vector for cloning human Sox2 with 6xHN tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 867)
  TITLE     McKinnon Lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 867)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pDNR-hSox2
FEATURES             Location/Qualifiers
     source          1..867
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     4..21
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    complement(33..66)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 cgcagcgagt cagtgagcga ggaagcggcc gcataacttc gtatagcata cattatacga
       61 agttatcagt cgacggtacc ggacatatgc ccgggaattc ggcacgaggc ccgggccccc
      121 caaagtcccg gccgggccga gggtcggcgg ccgccggcgg gccgggcccg cgcacagcgc
      181 ccgcatgtac aacatgatgg agacggagct gaagccgccg ggcccgcagc aaacttcggg
      241 gggcggcggc ggcaactcca ccgcggcggc ggccggcggc aaccagaaaa acagcccgga
      301 ccgcgtcaag cggcccatga atgccttcat ggtgtggtcc cgcgggcagc ggcgcaagat
      361 ggcccaggag aaccccaaga tgcacaactc ggagatcagc aagcgcctgg gcgccgagtg
      421 gaaacttttg tcggagacgg agaagcggcc gttcatcgac gaggctaagc ggctgcgagc
      481 gctgcacatg aaggagcacc cggattataa ataccggccc cggcggaaaa ccaagacgct
      541 catgaagaag gataagtaca cgctgcccgg cgggctgctg gcccccggcg gcaatagcat
      601 ggcgagcggg gtcggggtgg gcgccggcct gggcgcgggc gtgaaccagc gcatggacag
      661 ttacgcgcac atgaacggct ggagcaacgg cagctacagc atgatgcagg accagctggg
      721 ctacccgcag cacccgggcc tcaatgcgca cggcgcagcg cagatgcagc ccatgcaccg
      781 ctacgacgtg agcgccctgc agtacaactc catgaccagc tcgcagacct acatgaacgg
      841 ctcgcccacc tacagcatgt cctactc
//
