LOCUS       sequence-74714-e         978 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 978)
  AUTHORS   Stanton BC, Nielsen AA, Tamsir A, Clancy K, Peterson T, Voigt CA
  TITLE     Genomic mining of prokaryotic repressors for orthogonal logic gates.
  JOURNAL   Nat Chem Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411.
  PUBMED    24316737
REFERENCE   2  (bases 1 to 978)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411."
COMMENT     Sequence Label: pRF-AmtR
FEATURES             Location/Qualifiers
     source          1..978
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             38..49
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     terminator      complement(127..154)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      complement(170..241)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 cggtgaacag ctcctcgccc ttgctcacca tctagtattt ctcctctttc tctagtagct
       61 agcattatct atagatcgat agaaactgtc aattggtaac gaatcttcca ccgtacgtcg
      121 tataaacgca gaaaggccca cccgaaggtg agccagtgtg actctagtag agagcgttca
      181 ccgacaaaca acagataaaa cgaaaggccc agtctttcga ctgagccttt cgttttattt
      241 gatgcctggg ggtatgggcc ctagttattt tgcatctgcc tgtttaatca gttccagggt
      301 tttttcaaca cgatctgcag gcagcggtgc acccagaact gccaggcttg catctgccag
      361 cataattgcg gtttccggca ggctatctgc gctcagcgga ctcggaattt taccatcatt
      421 gcgacgcatt tcaataacgc tcatggtaat atgaaacggc agttctgcac gcggatcatc
      481 accaacaatt tcggttgcca gatcacgaaa aacattggtc agtgcttcac gctggctatg
      541 atattctgca aattcttcgc taccaacaat cggcagctga tacagacgac caacattcca
      601 tttggtgctc agcagcagac gaacttcgct tgcaacaatt gcccacagac gcatttccgg
      661 acctgcatcc agggtgctca gatcttctgc cagaacggtg ctcggttcaa cggtgctttt
      721 cagcagggtc agaaagattt cggttttgct cggaaaatga taatacaggc ttgcctgacg
      781 aataccaact gcatctgcaa tctgatgggt actggtggtt gcaaaaccct gacgggtaaa
      841 cagttctgcg cttgcatcca gaatttcttc acgcggattt ttacctgcac gacgcggtgc
      901 actacgacgc ggacgaccaa ctgcgcctgc catctagtat tctctctttc aaacatagtc
      961 catagaattg tgagcgct
//
