LOCUS       sequence-74715-e         976 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 976)
  AUTHORS   Stanton BC, Nielsen AA, Tamsir A, Clancy K, Peterson T, Voigt CA
  TITLE     Genomic mining of prokaryotic repressors for orthogonal logic gates.
  JOURNAL   Nat Chem Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411.
  PUBMED    24316737
REFERENCE   2  (bases 1 to 976)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411."
COMMENT     Sequence Label: pRF-McbR
FEATURES             Location/Qualifiers
     source          1..976
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             38..49
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     terminator      complement(127..154)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      complement(170..241)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     protein_bind    934..953
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
ORIGIN
        1 cggtgaacag ctcctcgccc ttgctcacca tctagtattt ctcctctttc tctagtagct
       61 agcattatat agacagatct ttctattgtc aattggtaac gaatcttcca ccgtacgtcg
      121 tataaacgca gaaaggccca cccgaaggtg agccagtgtg actctagtag agagcgttca
      181 ccgacaaaca acagataaaa cgaaaggccc agtctttcga ctgagccttt cgttttattt
      241 gatgcctggg ggtatgggcc ctagttaaat gctataatct gcaggcggtg cgctcagcag
      301 ctgacgtgcc agatcacgtg cggtttccag cggactaatg ttatgaacca gacggctacc
      361 tgccagacca ccatccagaa aaaccagcag ctgatttgcc tgggttgtac ccggataacc
      421 atttttttcg gtcagcagat cggtcagggt tttatgacac cattcacgat gttccagaac
      481 tgctgcaaca ataccttttt cgctatcggt ttccggacgc ggatattcgc ttgctgcatt
      541 ctgaaaatgg ctaccgcgaa aatctttttc cggttcttct tcgatgcact gatcaaagaa
      601 cgcaatgatt ttatcttccg gatctttcat accaacggta cgttcacgcc atgcttcacg
      661 ccacagctga tccagatttt ccagataggc aataaccagt gcatctttgc taccaaacag
      721 gctatacagg cttgcttttg caacatctgc ttcacgcaga atacgatcaa taccaatcac
      781 acgaatacct tcggtggtaa acaggttggt tgcgctatcc agcagacgct gacgcggact
      841 cggacgatta cgacgacgat ttgcaccggc actggttttg cttttaccgc ttgcgcttgc
      901 tgccatctag tatttctcct acatagtcca tagaattgtg agcgctcaca attccacaca
      961 ttatacgagc cgatga
//
