LOCUS       sequence-74724-e        8122 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expresses Membrane tagged G-CaMP3 in cells expressing Cre.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8122)
  AUTHORS   Vrontou S, Wong AM, Rau KK, Koerber HR, Anderson DJ
  TITLE     Genetic identification of C fibres that detect massage-like stroking
            of hairy skin in vivo.
  JOURNAL   Nature. 2013 Jan 31;493(7434):669-73. doi: 10.1038/nature11810.
  PUBMED    23364746
REFERENCE   2  (bases 1 to 8122)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2013-01-31- 31"; volume: "493"; issue: "7434"; 
            pages: "669-73"
COMMENT     Sequence Label: AAV-CMV-LOXP-stop-LOXP-mG-CaMP3.0
FEATURES             Location/Qualifiers
     source          1..8122
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   4..133
                     /label=AAV2 ITR
     enhancer        212..591
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        592..795
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     746..766
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     792..816
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          930..1405
                     /label=beta-globin intron
                     /note="internally truncated intron from human beta-globin"
     protein_bind    complement(1460..1493)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             1501..2295
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             1501..2295
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1555..1574)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     2165..2184
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(2483..2502)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    2527..2574
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     protein_bind    complement(2737..2770)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     regulatory      2799..2808
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2829..2837,2838..2855,2856..2858,2859..2891,2892..2894,
                     2895..2918,2919..2945,2946..3002,3003..3008,3009..3278,
                     3279..3296,3297..3731,3732..3734,3735..3737,3738..4181)
                     /codon_start=1
                     /product="improved fluorescent protein-based calcium sensor
                     (Chen et al., 2013)"
                     /label=GCaMP6f
                     /translation="MGSHHHHHHGMASMTGGQQMGRDLYDDDDKDLATMVDSSRRKWNK
                     TGHAVRAIGRLSSLENVYIKADKQKNGIKANFKIRHNIEDGGVQLAYHYQQNTPIGDGP
                     VLLPDNHYLSVQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGGTGGSMVSKGEE
                     LFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLT
                     YGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFKDDGNYKTRAEVKFEGDTLVNRIE
                     LKGIDFKEDGNILGHKLEYNTRDQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRS
                     LGQNPTEAELQDMINEVDADGDGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGN
                     GYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK"
     CDS             join(2829..2837,2838..2855,2856..2858,2859..2891,2892..2894,
                     2895..2918,2919..2945,2946..3002,3003..3008,3009..3278,
                     3279..3296,3297..3731,3732..3734,3735..3737,3738..4181)
                     /codon_start=1
                     /product="improved fluorescent protein-based calcium sensor
                     (Chen et al., 2013)"
                     /label=GCaMP6s
                     /translation="MGSHHHHHHGMASMTGGQQMGRDLYDDDDKDLATMVDSSRRKWNK
                     TGHAVRAIGRLSSLENVYIKADKQKNGIKANFKIRHNIEDGGVQLAYHYQQNTPIGDGP
                     VLLPDNHYLSVQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGGTGGSMVSKGEE
                     LFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLT
                     YGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFKDDGNYKTRAEVKFEGDTLVNRIE
                     LKGIDFKEDGNILGHKLEYNTRDQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRS
                     LGQNPTEAELQDMINEVDADGDGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGN
                     GYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK"
     regulatory      2925..2934
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     3215..3236
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(3342..3363)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3603..3622)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     rep_origin      4905..5418
                     /direction=RIGHT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4992..5011)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5202..5223
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5900..5919)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6019..6041
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6079..6097)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6165..6269
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6270..6338,6339..7130)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6488..6507)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7301..7889
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7790..7809
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cagctgcgcg ctcgctcgct cactgaggcc gcccgggcaa agcccgggcg tcgggcgacc
       61 tttggtcgcc cggcctcagt gagcgagcga gcgcgcagag agggagtggc caactccatc
      121 actaggggtt ccttgtagtt aatgattaac ccgccatgct acttatctac gtacatttat
      181 attggctcat gtccaacatt accgccatgt tgacattgat tattgactag ttattaatag
      241 taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt
      301 acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg
      361 acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat
      421 ttacggtaaa ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct
      481 attgacgtca atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg
      541 gactttccta cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg
      601 ttttggcagt acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc
      661 caccccattg acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa
      721 tgtcgtaaca actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc
      781 tatataagca gagctcgttt agtgaaccgt cagatcgcct ggagacgcca tccacgctgt
      841 tttgacctcc atagaagaca ccgggaccga tccagcctcc cctcgaagct ttacatgtgg
      901 taccgagctc ggatcctgag aacttcaggg tgagtctatg ggacccttga tgttttcttt
      961 ccccttcttt tctatggtta agttcatgtc ataggaaggg gagaagtaac agggtacaca
     1021 tattgaccaa atcagggtaa ttttgcattt gtaattttaa aaaatgcttt cttcttttaa
     1081 tatacttttt tgtttatctt atttctaata ctttccctaa tctctttctt tcagggcaat
     1141 aatgatacaa tgtatcatgc ctctttgcac cattctaaag aataacagtg ataatttctg
     1201 ggttaaggca atagcaatat ttctgcatat aaatatttct gcatataaat tgtaactgat
     1261 gtaagaggtt tcatattgct aatagcagct acaatccagc taccattctg cttttatttt
     1321 atggttggga taaggctgga ttattctgag tccaagctag gcccttttgc taatcatgtt
     1381 catacctctt atcttcctcc cacagctcct gggcaacgtg ctggtctgtg tgctggccca
     1441 tcactttggc aaagaattca taacttcgta tagcatacat tatacgaagt tatcgccacc
     1501 atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc
     1561 ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca
     1621 gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg
     1681 caagacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg
     1741 ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag
     1801 gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg
     1861 cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc
     1921 atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa
     1981 gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgag catgcccgac
     2041 ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat
     2101 ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac
     2161 atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc
     2221 ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt
     2281 gacgagttct tctgagcggg actctggggt tcgaaatgac cgaccaagcg acgcccaacc
     2341 tgccatcacg agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg
     2401 ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg
     2461 cccaccctag ggggaggcta actgaaacac ggaaggagac aataccggaa ggaacccgcg
     2521 ctatgacggc aataaaaaga cagaataaaa cgcacggtgt tgggtcgttt gttcataaac
     2581 gcggggttcg gtcccagggc tggcactctg tcgatacccc accgagaccc cattggggcc
     2641 aatacgcccg cgtttcttcc ttttccccac cccacccccc aagttcgggt gaaggcccag
     2701 ggctcgcagc caacgtcggg gcggcaggcc ctgccaataa cttcgtatag catacattat
     2761 acgaagttat cgtcggatca gctggcgcga gctggcgcgc caccatgggt tgttgcttct
     2821 ccaagaccat gggttctcat catcatcatc atcatggtat ggctagcatg actggtggac
     2881 agcaaatggg tcgggatctg tacgacgatg acgataagga tctcgccacc atggtcgact
     2941 catcacgtcg taagtggaat aagacaggtc acgcagtcag agctataggt cggctgagct
     3001 cactcgagaa cgtctatatc aaggccgaca agcagaagaa cggcatcaag gcgaacttca
     3061 agatccgcca caacatcgag gacggcggcg tgcagctcgc ctaccactac cagcagaaca
     3121 cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc gtgcagtcca
     3181 aactttcgaa agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg
     3241 ccgccgggat cactctcggc atggacgagc tgtacaaggg cggtaccgga gggagcatgg
     3301 tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg
     3361 acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca
     3421 agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg
     3481 tgaccaccct gacctacggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc
     3541 acgacttctt caagtccgcc atgcccgaag gctacatcca ggagcgcacc atcttcttca
     3601 aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga
     3661 accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc
     3721 tggagtacaa cacgcgtgac caactgactg aagagcagat cgcagaattt aaagaggctt
     3781 tctccctatt tgacaaggac ggggatggga caataacaac caaggagctg gggacggtga
     3841 tgcggtctct ggggcagaac cccacagaag cagagctgca ggacatgatc aatgaagtag
     3901 atgccgacgg tgacggcaca atcgacttcc ctgagttcct gacaatgatg gcaagaaaaa
     3961 tgaaagacac agacagtgaa gaagaaatta gagaagcgtt ccgtgtgttt gataaggatg
     4021 gcaatggcta catcagtgca gcagagcttc gccacgtgat gacaaacctt ggagagaagt
     4081 taacagatga agaggttgat gaaatgatca gggaagcaga catcgatggg gatggtcagg
     4141 taaactacga agagtttgta caaatgatga cagcgaagtg ataaggcgcg ccagctcgcg
     4201 ccagctatca agctcgagtg ctttatttgt gaaatttgtg atactattgc tttatttgta
     4261 accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt tattgtttca
     4321 ggttcagggg gaggtgtggg aggtttttta aagggggagg gggtacgtag ataagtagca
     4381 tggcgggtta atcattaact acaaggaacc cctagtgatg gagttggcca ctccctctct
     4441 gcgcgctcgc tcgctcactg aggccgggcg accaaaggtc gcccgacgcc cgggctttgc
     4501 ccgggcggcc tcagtgagcg agcgagcgcg ccagctggcg taatagcgaa gaggcccgca
     4561 ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atggcgattc tgttgcaatg
     4621 gctggcggta atattgttct ggatattacc agcaaggccg atagtttgag ttcttctact
     4681 caggcaagtg atgttattac taatcaaaga agtattgcga caacggttaa tttgcgtgat
     4741 ggacagactc ttttactcgg tggcctcact gattataaaa acacttctca ggattctggc
     4801 gtaccgttcc tgtctaaaat ccctttaatc ggcctcctgt ttagctcccg ctctgattct
     4861 aacgaggaaa gcacgttata cgtgctcgtc aaagcaacca tagtacgcgc cctgtagcgg
     4921 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
     4981 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
     5041 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
     5101 cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac
     5161 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
     5221 tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat
     5281 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa
     5341 aatattaacg tttacaattt aaatatttgc ttatacaatc ttcctgtttt tggggctttt
     5401 ctgattatca accggggtac atatgattga catgctagtt ttacgattac cgttcatcga
     5461 ttctcttgtt tgctccagac tctcaggcaa tgacctgata gcctttgtag agacctctca
     5521 aaaatagcta ccctctccgg catgaattta tcagctagaa cggttgaata tcatattgat
     5581 ggtgatttga ctgtctccgg cctttctcac ccgtttgaat ctttacctac acattactca
     5641 ggcattgcat ttaaaatata tgagggttct aaaaattttt atccttgcgt tgaaataaag
     5701 gcttctcccg caaaagtatt acagggtcat aatgtttttg gtacaaccga tttagcttta
     5761 tgctctgagg ctttattgct taattttgct aattctttgc cttgcctgta tgatttattg
     5821 gatgttggaa tgccctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc
     5881 gcatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac
     5941 acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca
     6001 gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga
     6061 aacgcgcgag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa
     6121 taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     6181 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     6241 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     6301 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     6361 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     6421 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     6481 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
     6541 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     6601 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     6661 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     6721 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     6781 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     6841 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     6901 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     6961 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     7021 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     7081 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     7141 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     7201 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     7261 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     7321 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     7381 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     7441 atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     7501 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     7561 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     7621 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     7681 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     7741 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     7801 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     7861 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     7921 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     7981 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     8041 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg
     8101 cgcgttggcc gattcattaa tg
//
