LOCUS       sequence-74727-e         902 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian expression of human Akt1 fused to EGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 902)
  TITLE     McKinnon Lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 902)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLPS-hAkt1-gfp
FEATURES             Location/Qualifiers
     source          1..902
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(31..64)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 ctggtttagt gaaccgtcag atccgctagc ataacttcgt atagcataca ttatacgaag
       61 ttatcagtcg acggtaccgg acatatgccc gggaattcag aggagcgcgt gagcgtcgcg
      121 ggagcctcgg gcaccatgag cgacgtggct attgtgaagg agggttggct gcacaaacga
      181 ggggagtaca tcaagacctg gcggccacgc tacttcctcc tcaagaatga tggcaccttc
      241 attggctaca aggagcggcc gcaggatgtg gaccaacgtg aggctcccct caacaacttc
      301 tctgtggcgc agtgccagct gatgaagacg gagcggcccc ggcccaacac cttcatcatc
      361 cgctgcctgc agtggaccac tgtcatcgaa cgcaccttcc atgtggagac tcctgaggag
      421 cgggaggagt ggacaaccgc catccagact gtggctgacg gcctcaagaa gcaggaggag
      481 gaggagatgg acttccggtc gggctcaccc agtgacaact caggggctga agagatggag
      541 gtgtccctgg ccaagcccaa gcaccgcgtg accatgaacg agtttgagta cctgaagctg
      601 ctgggcaagg gcactttcgg caaggtgatc ctggtgaagg agaaggccac aggccgctac
      661 tacgccatga agatcctcaa gaaggaagtc atcgtggcca aggacgaggt ggcccacaca
      721 ctcaccgaga accgcgtcct gcagaactcc aggcacccct tcctcacagc cctgaagtac
      781 tctttccaga cccacgaccg cctctgcttt gtcatggagt acgccaacgg gggcgagctg
      841 ttcttccacc tgtcccggga gcgtgtgttc tccgaggacc gggcccgctt ctatggcgct
      901 ga
//
