LOCUS       sequence-74731-e         838 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian bicistronic expression of human Sox2 and EGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 838)
  TITLE     McKinnon Lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 838)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLP-hSox2-ires-gfp
FEATURES             Location/Qualifiers
     source          1..838
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(70..103)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 gctggtttag tgaaccgtca gatccgctag cgctaccgga ctcagatctc gagctcaagc
       61 ttcgaattca taacttcgta tagcatacat tatacgaagt tatcagtcga cggtaccgga
      121 catatgcccg ggaattcggc acgaggcccg ggccccccaa agtcccggcc gggccgaggg
      181 tcggcggccg ccggcgggcc gggcccgcgc acagcgcccg catgtacaac atgatggaga
      241 cggagctgaa gccgccgggc ccgcagcaaa cttcgggggg cggcggcggc aactccaccg
      301 cggcggcggc cggcggcaac cagaaaaaca gcccggaccg cgtcaagcgg cccatgaatg
      361 ccttcatggt gtggtcccgc gggcagcggc gcaagatggc ccaggagaac cccaagatgc
      421 acaactcgga gatcagcaag cgcctgggcg ccgagtggaa acttttgtcg gagacggaga
      481 agcggccgtt catcgacgag gctaagcggc tgcgagcgct gcacatgaag gagcacccgg
      541 attataaata ccggccccgg cggaaaacca agacgctcat gaagaaggat aagtacacgc
      601 tgcccggcgg gctgctggcc cccggcggca atagcatggc gagcggggtc ggggtgggcg
      661 ccggcctggg cgcgggcgtg aaccagcgca tggacagtta cgcgcacatg aacggctgga
      721 gcaacggcag ctacagcatg atgcaggacc agctgggcta cccgcagcac ccgggcctca
      781 atgcgcacgg cgcagcgcag atgcagccca tgcaccgcta cgacgtgagc gccctgca
//
