LOCUS       sequence-74733-e         945 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian bicistronic expression of mouse Oct3/4 and EGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 945)
  TITLE     McKinnon Lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 945)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLP-mOct4-ires-gfp
FEATURES             Location/Qualifiers
     source          1..945
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(68..101)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 ctggtttagt gaccgtcaga tccgctagcg ctaccggact cagatctcga gctcaagctt
       61 cgaattcata acttcgtata gcatacatta tacgaagtta tcagtcgacg gtaccggaca
      121 tatgcccggg tcgacaagag gtaatccacc cccggctcgg ggtgcccacc ttccccatgg
      181 ctggacacct ggcttcagac ttcgccttct cacccccacc aggtgggggt gatgggtcag
      241 cagggctgga gccgggctgg gtggatcctc gaacctggct aagcttccaa gggcctccag
      301 gtgggcctgg aatcggacca ggctcagagg tattggggat ctccccatgt ccgcccgcat
      361 acgagttctg cggagggatg gcatactgtg gacctcaggt tggactgggc ctagtccccc
      421 aagttggcgt ggagactttg cagcctgagg gccaggcagg agcacgagtg gaaagcaact
      481 cagagggaac ctcctctgag ccctgtgccg accgccccaa tgccgtgaag ttggagaagg
      541 tggaaccaac tcccgaggag tcccaggaca tgaaagccct gcagaaggag ctagaacagt
      601 ttgccaagct gctgaagcag aagaggatca ccttggggta cacccaggcc gacgtggggc
      661 tcaccctggg cgttctcttt ggaaaggtgt tcagccagac caccatctgt cgcttcgagg
      721 ccttgcagct cagccttaag aacatgtgta agctgcggcc cctgctggag aagtgggtgg
      781 aggaagccga caacaatgag aaccttcagg agatatgcaa atcggagacc ctggtgcagg
      841 cccggaagag aaagcgaact agcattgaga accgtgtgag gtggagtctg gagaccatgt
      901 ttctgaagtg cccgaagccc tccctacagc agatcactca catcg
//
