LOCUS       sequence-74740-e         900 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  mammalian bicistronic expression of mouse Olig2 and EGFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 900)
  TITLE     McKinnon Lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 900)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLP-mOlig2-ires-gfp
FEATURES             Location/Qualifiers
     source          1..900
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(71..104)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 agctggttta gtgaaccgtc agatccgcta gcgctaccgg actcagatct cgagctcaag
       61 cttcgaattc ataacttcgt atagcataca ttatacgaag ttatcagtcg acggtaccgg
      121 acatatgccc gggaattcgg cacgaggcca gcggaacccc gaaaggtgtg gatgcttatt
      181 acagaccgag ccaacaccag cgcccagagc caggttctcc tccgcagcga gcacctcaaa
      241 tctaattcac attcggaagg ttgaaaaaag aaggatcatt cgagagctta gatcatccct
      301 ggggccatgg actcggacgc cagcctggtg tctagtcgcc catcgtcccc agaacccgat
      361 gatctttttc tgcccgcccg gagcaagggg ggaagcagca gcggcttcac aggagggact
      421 gtgtcctcgt ccacgccgag cgactgcccg ccagagctga gctccgagct acgaggggcc
      481 atgggcgcgt caggcgcgca tcccggggac aaactgggcg gcggtggctt caagtcatct
      541 tcctccagca cctcctcgtc cacgtcctcg gcggccacgt cttccaccaa gaaagacaag
      601 aagcagatga ctgagcccga gctgcagcag ctgcgcctga agatcaacag ccgcgaacgc
      661 aagcgcatgc acgacctcaa catcgccatg gacggcctgc gggaggtcat gccttacgcg
      721 cacgggccgt cggtgcgcaa gctctccaag atcgccacgc tgctgctggc gcgaaactac
      781 atcctcatgc tcaccaactc gctggaggag atgaagcgac tggtgagcga gatctacggg
      841 ggtcaccacg ccggcttcca cccatcggcc tgtggcgggc tggcacactc ggcgcctcta
//
