LOCUS       sequence-74741-e         986 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 986)
  AUTHORS   Stanton BC, Nielsen AA, Tamsir A, Clancy K, Peterson T, Voigt CA
  TITLE     Genomic mining of prokaryotic repressors for orthogonal logic gates.
  JOURNAL   Nat Chem Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411.
  PUBMED    24316737
REFERENCE   2  (bases 1 to 986)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411."
COMMENT     Sequence Label: pRF-SmcR
FEATURES             Location/Qualifiers
     source          1..986
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             39..50
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     terminator      complement(145..172)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      complement(188..259)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     RBS             907..918
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     protein_bind    935..954
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
ORIGIN
        1 ccggtgaaca gctcctcgcc cttgctcacc atctagtatt tctcctcttt ctctagtagc
       61 tagcatttta cgcagattta tcaataatgt caattggtaa cgaatcagac aattgacggc
      121 tcgttccacc gtacgtcgta taaacgcaga aaggcccacc cgaaggtgag ccagtgtgac
      181 tctagtagag agcgttcacc gacaaacaac agataaaacg aaaggcccag tctttcgact
      241 gagcctttcg ttttatttga tgcctggggg tatgggccct agttattcgt gttcgcgttt
      301 atagatacac agcatatcca gatagctgct aaccagtttg ctcagttctg cggtgttatt
      361 ggtgcgattt gcctgaacaa acaggctata gcaaatacca tgaaacaggt ttgccagatc
      421 ttccggatta tgctgatcgc aaacttcgcc acgttcaatt gctttgataa acatgttctg
      481 aaccagcagc tgattggtac gattggtggt aacaaacaga ggccaaactt catcacgggt
      541 gcttgctgac cattcaaacc aaactttcag ccaatgatta tcctgaacca ccagttcaat
      601 catggcgttg gtaatattgg caatgttttc tttggcatgc agatccagat cgatattatc
      661 gctcaggaaa ttgctaaact ggcgaacaac atgattcaga acttcatcaa ccagatcttc
      721 acgggtcgga aaatagttaa acacggttgc aacgctaacc tgtgcaattt ctgcaatatc
      781 tgcatgacca ccacgaccaa taccacgacg tgcaaaaact tccagggcaa tttccatcag
      841 ctgctgttta cgtttcagcg gactcagacg ggtacgcgga cgttttgcaa tgctatccat
      901 ctagtatttc tcctctttca aacatagtcc atagaattgt gagcgctcac aattccacac
      961 attatacgag ccgatgatta attgtc
//
