LOCUS       sequence-74748-e         950 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  donor vector for cloning human Akt1 with 6xHN tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 950)
  TITLE     McKinnon Lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 950)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pDNR-hAkt1
FEATURES             Location/Qualifiers
     source          1..950
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      55..149
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(185..220)
                     /codon_start=1
                     /product="6x(His-Asn) affinity tag"
                     /label=6xHN
                     /translation="HNHNHNHNHNHN"
ORIGIN
        1 acagtactgc gatgagtggc agggcggggc gtaatttgat atcgagctcg cttggactcc
       61 tgttgataga tccagtaatg acctcagaac tccatctgga tttgttcaga acgctcggtt
      121 gccgccgggc gttttttatt ggtgagaatc caagcactag tcatgaccag gatctcctag
      181 gctagttgtg attatgatta tgattatgat tatgattatg accacttacc tgggcccgcg
      241 cgccaaacga atggtctaga aagcttctcg agcggatccc gccgcctggg gccgtgccgc
      301 tggccgagta ggagaactgg gggaagtggg gcctgcgctc gctgtccaca cactccatgc
      361 tgtcatcttg gtcaggtggt gtgatggtga tcatctgggc cgtgaactcc tcatcaaaat
      421 acctggtgtc agtctccgac gtgacctggg gcttgaaggg tgggctgagc ttcttctcgt
      481 acacgtgctg ccacacgata ccggcaaaga agcgatgctg catgatctcc ttggcgtcct
      541 cggagccccc gccaagcctc tgcttggggt ccttcttgag cagccctgaa agcaaggact
      601 tggcctcggg accaagcgtg cgcgggaagc ggatctcctc catgaggatg agctcaaaaa
      661 gcttctcatg gtcctggttg tagaagggca ggcgaccgca catcatctcg tacatgacca
      721 cgcccagccc ccaccagtcc actgcacggc cgtagtcatt gtcctccagc acctcggggg
      781 ccaggtactc aggtgtgccg caaaaggtct tcatggtggc accgtccttg atcccctcct
      841 tgcacagccc gaagtctgtg atcttaatgt gcccgtcctt gtccagcatg aggttctcca
      901 gcttgaggtc ccggtacacc acgttcttct ccgagtgcag gtagtccagg
//
