LOCUS       sequence-74754-e         999 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 999)
  AUTHORS   Stanton BC, Nielsen AA, Tamsir A, Clancy K, Peterson T, Voigt CA
  TITLE     Genomic mining of prokaryotic repressors for orthogonal logic gates.
  JOURNAL   Nat Chem Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411.
  PUBMED    24316737
REFERENCE   2  (bases 1 to 999)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411."
COMMENT     Sequence Label: pRF-PhlF
FEATURES             Location/Qualifiers
     source          1..999
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             39..50
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     terminator      complement(131..158)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      complement(174..245)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     protein_bind    905..924
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     promoter        complement(join(930..936,937..952,953..958))
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
ORIGIN
        1 ccggtgaaca gctcctcgcc cttgctcacc atctagtatt tctcctcttt ctctagtaac
       61 cttaacgata cggtacgttt cgtatcatgt caattggtaa cgaatcagat tccaccgtac
      121 gtcgtataaa cgcagaaagg cccacccgaa ggtgagccag tgtgactcta gtagagagcg
      181 ttcaccgaca aacaacagat aaaacgaaag gcccagtctt tcgactgagc ctttcgtttt
      241 atttgatgcc tgggggtatg ggccctagtt aacgctgtgt acccggacaa acaccattaa
      301 tcagcaggaa ggtaaattct tcaatatcct gttcaacggt cagctgttcg gtcagcaggc
      361 gataccaaca aaaaccaaaa atcatatcca gcagcagttc acgattggta tctttcggca
      421 gttcaccatt gctaatggca ttttcaacca gttttttcgg catctcacga cgacgttcca
      481 taaactgatc tttcagctgg gtcagggttg cagggtccag ctgtgcttct gcaataacac
      541 aacgaaatgc ttcaccacaa atggtttcac gccaaacttt ccacagatta cgcagcagaa
      601 aatccagatc ggctttaaag ctacccagat ccggaaattt acgcacctgt tcgctttcat
      661 tttcatacac ttcggcaatc agtgctgctt tattggtcca ccaacgataa atggtcggtt
      721 tgcttgcacc ggcacgacgt gcaacgcttt caatgctcag accgctataa ccacattctt
      781 tcaggatttc aatggtgctg gtcagaattg ctttatgggt atgcggacta cgcaggctac
      841 caatgctgct acggctcggg gtacgtgcca tctagtattt ctccctttca aacatagtcc
      901 atagaattgt gagcgctcac aattccacac attatacgag ccgatgatta attgtcaaca
      961 cagccaacga tcgttccgat cagaacaaga aagctatgt
//
