LOCUS       sequence-74760-e         995 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 995)
  AUTHORS   Stanton BC, Nielsen AA, Tamsir A, Clancy K, Peterson T, Voigt CA
  TITLE     Genomic mining of prokaryotic repressors for orthogonal logic gates.
  JOURNAL   Nat Chem Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411.
  PUBMED    24316737
REFERENCE   2  (bases 1 to 995)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411."
COMMENT     Sequence Label: pRF-ScbR
FEATURES             Location/Qualifiers
     source          1..995
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             39..50
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     terminator      complement(147..174)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      complement(190..261)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     protein_bind    966..985
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
ORIGIN
        1 ccggtgaaca gctcctcgcc cttgctcacc atctagtatt tctcctcttt ctctagtaaa
       61 cataccatta tagcggtatg atagctagct gtcaattggt aacgaatcag acaattgacg
      121 gctcgttcca ccgtacgtcg tataaacgca gaaaggccca cccgaaggtg agccagtgtg
      181 actctagtag agagcgttca ccgacaaaca acagataaaa cgaaaggccc agtctttcga
      241 ctgagccttt cgttttattt gatgcctggg ggtatgggcc ctagttaatc tttaccggtc
      301 ggtgccagtt ctgctgccag acgtgcacca cgttcttcgc tcagatccag tgctgccaga
      361 acgctcggaa ctgcaattgc aggcagaata tgtttctgca gcagtgcata acgatgttcc
      421 agatcctgat aatcgctaac ggtctggcta acaacctgaa tacctgcaaa ggtgcccaca
      481 tacagatctg cgctatcggt ggtaacaaca tgcggcagca gttcaccatt ttcttttgcc
      541 tgattcagca gtttcagcag ggtttcatgc caacgacgaa acggaccacg acgatccaga
      601 ccatgtgcct gctgatccat gctcagacga acaccggcac gtgcaacaac attggtacgc
      661 agacgatgac aaaacagcat acccatatca atcagttctt gcagacgcag cggctgttcc
      721 ggaactgcct gaggcggttc ttgtgcatca aaaacgccca gtgccagttc ttctttgctc
      781 tgaaagtgaa aatacagtgc acctttggta acaccggcaa ctttcagaat ttcggtaatg
      841 gttgctgcct gataaccctg tttttcaaaa acctgtgctg ctgcatccag aatggtctga
      901 cgggtacgaa ttgcacgatc ctgttttgcc atctagtact tttcctcttt aaacatagtc
      961 catagaattg tgagcgctca caattccaca catta
//
