LOCUS       sequence-74772-e         935 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  donor vector for cloning human Sox2 with 6xHN tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 935)
  TITLE     McKinnon Lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 935)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pDNR-hSox2
FEATURES             Location/Qualifiers
     source          1..935
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      52..146
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(182..217)
                     /codon_start=1
                     /product="6x(His-Asn) affinity tag"
                     /label=6xHN
                     /translation="HNHNHNHNHNHN"
ORIGIN
        1 gtactgcgat gagtggcagg gcggggcgta atttgatatc gagctcgctt ggactcctgt
       61 tgatagatcc agtaatgacc tcagaactcc atctggattt gttcagaacg ctcggttgcc
      121 gccgggcgtt ttttattggt gagaatccaa gcactagtca tgaccaggat ctcctaggct
      181 agttgtgatt atgattatga ttatgattat gattatgacc acttacctgg gcccgcgcgc
      241 caaacgaatg gtctagaaag cttggcatgt gtgagagggg cagtgtgccg ttaatggccg
      301 tgccgggcac cgggccgctc tggtagtgct gggacatgtg aagtctgctg ggggcggcgg
      361 gttccggcac ctcggcgccg gggagataca tgctgatcat gtcccggagg tccccggcct
      421 ggcagggcgc cctggagtgg gaggaagagg taaccacagg ggggctggag ctggcctcgg
      481 acttgaccac cgaacccatg gagccaagag ccatgccagg ggtgccctgc tgcgagtagg
      541 acatgctgta ggtgggcgag ccgttcatgt aggtctgcga gctggtcatg gagttgtact
      601 gcagggcgct cacgtcgtag cggtgcatgg gctgcatctg cgctgcgccg tgcgcattga
      661 ggcccgggtg ctgcgggtag cccagctggt cctgcatcat gctgtagctg ccgttgctcc
      721 agccgttcat gtgcgcgtaa ctgtccatgc gctggttcac gcccgcgccc aggccggcgc
      781 ccaccccgac cccgctcgcc atgctattgc cgccgggggc cagcagcccg ccgggcagcg
      841 tgtacttatc cttcttcatg agcgtcttgg ttttccgccg gggccggtat ttataatccg
      901 ggtgctcctt catgtgcagc gctcgcagcc gctta
//
