LOCUS       sequence-74773-e        8961 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  TALEColor-mCherry-Telomere.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8961)
  AUTHORS   Ma H, Reyes-Gutierrez P, Pederson T
  TITLE     Visualization of repetitive DNA sequences in human chromosomes with 
            transcription activator-like effectors.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Dec 9.
  PUBMED    24324157
REFERENCE   2  (bases 1 to 8961)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2013 Dec 9."
COMMENT     Sequence Label: pTH-TelR20-mCherry
FEATURES             Location/Qualifiers
     source          1..8961
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(43..62)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        234..613
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        614..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     768..788
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    819..837
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    840..858
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     868..892
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      1004..1013
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1013..1078
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             1085..1105
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3644..4354
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(3789..3807)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     4057..4076
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     complement(4386..4403)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4392..4616
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4662..5090
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4749..4768)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4959..4980
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5099..5119)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5104..5433
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5284..5419
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5346..5365
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5482..6507
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             5482..6507
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     polyA_signal    6637..6758
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6674..6693)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6728..6747
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6807..6823)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6807..6823)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6820..6842)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6831..6847
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6855..6861,6862..6879,6880..6885))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6900..6921
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7038..7055)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7209..7794)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7289..7308)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7965..8756,8757..8825))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8588..8607
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8826..8930)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 acggatcggg agatctcccg atcccctatg gtgcactctc agtacaatct gctctgatgc
       61 cgcatagtta agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc
      121 gagcaaaatt taagctacaa caaggcaagg cttgaccgac aattccatga agaatctgct
      181 tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc agatatacgc gttgacattg
      241 attattgact agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat
      301 ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
      361 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
      421 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta
      481 tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
      541 tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat
      601 cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga
      661 ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca
      721 aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg
      781 taggcgtgta cggtgggagg tctatataag cagagctctc cctatcagtg atagagatct
      841 ccctatcagt gatagagatc gtcgacgagc tcgtttagtg aaccgtcaga tcgcctggag
      901 acgccatcca cgctgttttg acctccatag aagacaccgg gaccgatcca gcctggcgcg
      961 ccggttctgg atccggttct caattgggtt ctaccggtta ggggccacca tggactataa
     1021 ggaccacgac ggagactaca aggatcatga tattgattac aaagacgatg acgataagat
     1081 ggccccaaag aagaagcgga aggtcggtat ccacggagtc ccagcagccg tagatttgag
     1141 aactttggga tattcacagc agcagcagga aaagatcaag cccaaagtga ggtcgacagt
     1201 cgcgcagcat cacgaagcgc tggtgggtca tgggtttaca catgcccaca tcgtagcctt
     1261 gtcgcagcac cctgcagccc ttggcacggt cgccgtcaag taccaggaca tgattgcggc
     1321 gttgccggaa gccacacatg aggcgatcgt cggtgtgggg aaacagtgga gcggagcccg
     1381 agcgcttgag gccctgttga cggtcgcggg agagctgaga gggcctcccc ttcagctgga
     1441 cacgggccag ttgctgaaga tcgcgaagcg gggaggagtc acggcggtcg aggcggtgca
     1501 cgcgtggcgc aatgcgctca cgggagcacc cctcaacctg accccagagc aggtcgtggc
     1561 aattgcgagc aacatcgggg gaaagcaggc actcgaaacc gtccagaggt tgctgcctgt
     1621 gctgtgccaa gcgcacggac ttacgccaga gcaggtcgtg gcaattgcga gcaacatcgg
     1681 gggaaagcag gcactcgaaa ccgtccagag gttgctgcct gtgctgtgcc aagcgcacgg
     1741 actaacccca gagcaggtcg tggcaattgc gagccatgac gggggaaagc aggcactcga
     1801 aaccgtccag aggttgctgc ctgtgctgtg ccaagcgcac gggttgaccc cagagcaggt
     1861 cgtggcaatt gcgagccatg acgggggaaa gcaggcactc gaaaccgtcc agaggttgct
     1921 gcctgtgctg tgccaagcgc acggcctgac cccagagcag gtcgtggcaa ttgcgagcca
     1981 tgacggggga aagcaggcac tcgaaaccgt ccagaggttg ctgcctgtgc tgtgccaagc
     2041 gcacggactg acaccagagc aggtcgtggc aattgcgagc aacggagggg gaaagcaggc
     2101 actcgaaacc gtccagaggt tgctgcctgt gctgtgccaa gcgcacggac ttacacccga
     2161 acaagtcgtg gcaattgcga gcaacatcgg gggaaagcag gcactcgaaa ccgtccagag
     2221 gttgctgcct gtgctgtgcc aagcgcacgg acttacgcca gagcaggtcg tggcaattgc
     2281 gagcaacatc gggggaaagc aggcactcga aaccgtccag aggttgctgc ctgtgctgtg
     2341 ccaagcgcac ggactaaccc cagagcaggt cgtggcaatt gcgagccatg acgggggaaa
     2401 gcaggcactc gaaaccgtcc agaggttgct gcctgtgctg tgccaagcgc acgggttgac
     2461 cccagagcag gtcgtggcaa ttgcgagcca tgacggggga aagcaggcac tcgaaaccgt
     2521 ccagaggttg ctgcctgtgc tgtgccaagc gcacggcctg accccagagc aggtcgtggc
     2581 aattgcgagc catgacgggg gaaagcaggc actcgaaacc gtccagaggt tgctgcctgt
     2641 gctgtgccaa gcgcacggac tgacaccaga gcaggtcgtg gcaattgcga gcaacggagg
     2701 gggaaagcag gcactcgaaa ccgtccagag gttgctgcct gtgctgtgcc aagcgcacgg
     2761 cctcacccca gagcaggtcg tggcaattgc gagcaacatc gggggaaagc aggcactcga
     2821 aaccgtccag aggttgctgc ctgtgctgtg ccaagcgcac ggacttacgc cagagcaggt
     2881 cgtggcaatt gcgagcaaca tcgggggaaa gcaggcactc gaaaccgtcc agaggttgct
     2941 gcctgtgctg tgccaagcgc acggactaac cccagagcag gtcgtggcaa ttgcgagcca
     3001 tgacggggga aagcaggcac tcgaaaccgt ccagaggttg ctgcctgtgc tgtgccaagc
     3061 gcacgggttg accccagagc aggtcgtggc aattgcgagc catgacgggg gaaagcaggc
     3121 actcgaaacc gtccagaggt tgctgcctgt gctgtgccaa gcgcacggcc tgaccccaga
     3181 gcaggtcgtg gcaattgcga gccatgacgg gggaaagcag gcactcgaaa ccgtccagag
     3241 gttgctgcct gtgctgtgcc aagcgcacgg actgacacca gagcaggtcg tggcaattgc
     3301 gagcaacgga gggggaaagc aggcactcga aaccgtccag aggttgctgc ctgtgctgtg
     3361 ccaagcgcac ggactcacgc ctgagcaggt agtggctatt gcatccaaca tcgggggcag
     3421 acccgcactg gagtcaatcg tggcccagct ttcgaggccg gaccccgcgc tggccgcact
     3481 cactaatgat catcttgtag cgctggcctg cctcggcgga cgacccgcct tggatgcggt
     3541 gaagaagggg ctcccgcacg cgcctgcatt gattaagcgg accaacagaa ggattcccga
     3601 gaggacatca catcgagtgg caggcctgca gggaagtgga agtatggtga gcaagggcga
     3661 ggaggataac atggccatca tcaaggagtt catgcgcttc aaggtgcaca tggagggctc
     3721 cgtgaacggc cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac
     3781 ccagaccgcc aagctgaagg tgaccaaggg tggccccctg cccttcgcct gggacatcct
     3841 gtcccctcag ttcatgtacg gctccaaggc ctacgtgaag caccccgccg acatccccga
     3901 ctacttgaag ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga
     3961 cggcggcgtg gtgaccgtga cccaggactc ctcactgcag gacggcgagt tcatctacaa
     4021 ggtgaagctg cgcggcacca acttcccctc cgacggcccc gtaatgcaga agaagaccat
     4081 gggctgggag gcctcctccg agcggatgta ccccgaggac ggcgccctga agggcgagat
     4141 caagcagagg ctgaagctga aggacggcgg ccactacgac gctgaggtca agaccaccta
     4201 caaggccaag aagcccgtgc agctgcccgg cgcctacaac gtcaacatca agttggacat
     4261 cacctcccac aacgaggact acaccatcgt ggaacagtac gaacgcgctg agggccgcca
     4321 ctccaccggc ggcatggacg agctgtacaa gtagtctaga gggcccgttt aaacccgctg
     4381 atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc
     4441 ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc
     4501 atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa
     4561 gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc
     4621 tgaggcggaa agaaccagct ggggctctag ggggtatccc cacgcgccct gtagcggcgc
     4681 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
     4741 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
     4801 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
     4861 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
     4921 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
     4981 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc
     5041 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt aattctgtgg
     5101 aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     5161 agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc cccagcaggc
     5221 agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc cctaactccg
     5281 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
     5341 ttttttattt atgcagaggc cgaggccgcc tctgcctctg agctattcca gaagtagtga
     5401 ggaggctttt ttggaggcct aggcttttgc aaaaagctcc cgggagcttg tatatccatt
     5461 ttcggatctg atcagcacgt gatgaaaaag cctgaactca ccgcgacgtc tgtcgagaag
     5521 tttctgatcg aaaagttcga cagcgtctcc gacctgatgc agctctcgga gggcgaagaa
     5581 tctcgtgctt tcagcttcga tgtaggaggg cgtggatatg tcctgcgggt aaatagctgc
     5641 gccgatggtt tctacaaaga tcgttatgtt tatcggcact ttgcatcggc cgcgctcccg
     5701 attccggaag tgcttgacat tggggaattc agcgagagcc tgacctattg catctcccgc
     5761 cgtgcacagg gtgtcacgtt gcaagacctg cctgaaaccg aactgcccgc tgttctgcag
     5821 ccggtcgcgg aggccatgga tgcgatcgct gcggccgatc ttagccagac gagcgggttc
     5881 ggcccattcg gaccgcaagg aatcggtcaa tacactacat ggcgtgattt catatgcgcg
     5941 attgctgatc cccatgtgta tcactggcaa actgtgatgg acgacaccgt cagtgcgtcc
     6001 gtcgcgcagg ctctcgatga gctgatgctt tgggccgagg actgccccga agtccggcac
     6061 ctcgtgcacg cggatttcgg ctccaacaat gtcctgacgg acaatggccg cataacagcg
     6121 gtcattgact ggagcgaggc gatgttcggg gattcccaat acgaggtcgc caacatcttc
     6181 ttctggaggc cgtggttggc ttgtatggag cagcagacgc gctacttcga gcggaggcat
     6241 ccggagcttg caggatcgcc gcggctccgg gcgtatatgc tccgcattgg tcttgaccaa
     6301 ctctatcaga gcttggttga cggcaatttc gatgatgcag cttgggcgca gggtcgatgc
     6361 gacgcaatcg tccgatccgg agccgggact gtcgggcgta cacaaatcgc ccgcagaagc
     6421 gcggccgtct ggaccgatgg ctgtgtagaa gtactcgccg atagtggaaa ccgacgcccc
     6481 agcactcgtc cgagggcaaa ggaatagcac gtgctacgag atttcgattc caccgccgcc
     6541 ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag
     6601 cgcggggatc tcatgctgga gttcttcgcc caccccaact tgtttattgc agcttataat
     6661 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
     6721 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat accgtcgacc
     6781 tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
     6841 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
     6901 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
     6961 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
     7021 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     7081 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     7141 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     7201 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     7261 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     7321 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     7381 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     7441 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     7501 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     7561 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     7621 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     7681 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     7741 agcggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     7801 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     7861 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     7921 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     7981 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     8041 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     8101 ccgcgagaac cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     8161 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     8221 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     8281 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     8341 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     8401 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     8461 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     8521 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     8581 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     8641 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     8701 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     8761 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     8821 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     8881 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     8941 cgaaaagtgc cacctgacgt c
//
