LOCUS       sequence-74777-e        8970 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  TALEColor-mVenus-Chromosome 18-specific Centromere .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8970)
  AUTHORS   Ma H, Reyes-Gutierrez P, Pederson T
  TITLE     Visualization of repetitive DNA sequences in human chromosomes with 
            transcription activator-like effectors.
  JOURNAL   Proc Natl Acad Sci U S A. 2013 Dec 9.
  PUBMED    24324157
REFERENCE   2  (bases 1 to 8970)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2013 Dec 9."
COMMENT     Sequence Label: pTH-Cen18-mVenus
FEATURES             Location/Qualifiers
     source          1..8970
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(43..62)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        234..613
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        614..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     768..788
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    819..837
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    840..858
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     868..892
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      1004..1013
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1013..1078
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             1085..1105
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(3644..3646,3647..3649,3650..4363)
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002; Kremers et al., 2006)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3689..3710)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3950..3969)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4297..4318
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(4395..4412)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4401..4625
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4671..5099
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4758..4777)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4968..4989
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5108..5128)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5113..5442
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5293..5428
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5355..5374
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5491..6516
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             5491..6516
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     polyA_signal    6646..6767
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6683..6702)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6737..6756
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6816..6832)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6816..6832)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6829..6851)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6840..6856
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6864..6870,6871..6888,6889..6894))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6909..6930
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7047..7064)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7218..7803)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7298..7317)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7974..8765,8766..8834))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8597..8616
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8835..8939)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 acggatcggg agatctcccg atcccctatg gtgcactctc agtacaatct gctctgatgc
       61 cgcatagtta agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc
      121 gagcaaaatt taagctacaa caaggcaagg cttgaccgac aattccatga agaatctgct
      181 tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc agatatacgc gttgacattg
      241 attattgact agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat
      301 ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
      361 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
      421 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta
      481 tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
      541 tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat
      601 cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat agcggtttga
      661 ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt tttggcacca
      721 aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc aaatgggcgg
      781 taggcgtgta cggtgggagg tctatataag cagagctctc cctatcagtg atagagatct
      841 ccctatcagt gatagagatc gtcgacgagc tcgtttagtg aaccgtcaga tcgcctggag
      901 acgccatcca cgctgttttg acctccatag aagacaccgg gaccgatcca gcctggcgcg
      961 ccggttctgg atccggttct caattgggtt ctaccggtta ggggccacca tggactataa
     1021 ggaccacgac ggagactaca aggatcatga tattgattac aaagacgatg acgataagat
     1081 ggccccaaag aagaagcgga aggtcggtat ccacggagtc ccagcagccg tagatttgag
     1141 aactttggga tattcacagc agcagcagga aaagatcaag cccaaagtga ggtcgacagt
     1201 cgcgcagcat cacgaagcgc tggtgggtca tgggtttaca catgcccaca tcgtagcctt
     1261 gtcgcagcac cctgcagccc ttggcacggt cgccgtcaag taccaggaca tgattgcggc
     1321 gttgccggaa gccacacatg aggcgatcgt cggtgtgggg aaacagtgga gcggagcccg
     1381 agcgcttgag gccctgttga cggtcgcggg agagctgaga gggcctcccc ttcagctgga
     1441 cacgggccag ttgctgaaga tcgcgaagcg gggaggagtc acggcggtcg aggcggtgca
     1501 cgcgtggcgc aatgcgctca cgggagcacc cctcaacctg accccagagc aggtcgtggc
     1561 aattgcgagc aacggagggg gaaagcaggc actcgaaacc gtccagaggt tgctgcctgt
     1621 gctgtgccaa gcgcacggac ttacgccaga gcaggtcgtg gcaattgcga gcaaccacgg
     1681 gggaaagcag gcactcgaaa ccgtccagag gttgctgcct gtgctgtgcc aagcgcacgg
     1741 actaacccca gagcaggtcg tggcaattgc gagcaacatc gggggaaagc aggcactcga
     1801 aaccgtccag aggttgctgc ctgtgctgtg ccaagcgcac gggttgaccc cagagcaggt
     1861 cgtggcaatt gcgagcaaca tcgggggaaa gcaggcactc gaaaccgtcc agaggttgct
     1921 gcctgtgctg tgccaagcgc acggcctgac cccagagcag gtcgtggcaa ttgcgagcca
     1981 tgacggggga aagcaggcac tcgaaaccgt ccagaggttg ctgcctgtgc tgtgccaagc
     2041 gcacggactg acaccagagc aggtcgtggc aattgcgagc catgacgggg gaaagcaggc
     2101 actcgaaacc gtccagaggt tgctgcctgt gctgtgccaa gcgcacggac ttacacccga
     2161 acaagtcgtg gcaattgcga gcaacatcgg gggaaagcag gcactcgaaa ccgtccagag
     2221 gttgctgcct gtgctgtgcc aagcgcacgg acttacgcca gagcaggtcg tggcaattgc
     2281 gagccatgac gggggaaagc aggcactcga aaccgtccag aggttgctgc ctgtgctgtg
     2341 ccaagcgcac ggactaaccc cagagcaggt cgtggcaatt gcgagccatg acgggggaaa
     2401 gcaggcactc gaaaccgtcc agaggttgct gcctgtgctg tgccaagcgc acgggttgac
     2461 cccagagcag gtcgtggcaa ttgcgagcaa ccacggggga aagcaggcac tcgaaaccgt
     2521 ccagaggttg ctgcctgtgc tgtgccaagc gcacggcctg accccagagc aggtcgtggc
     2581 aattgcgagc aacggagggg gaaagcaggc actcgaaacc gtccagaggt tgctgcctgt
     2641 gctgtgccaa gcgcacggac tgacaccaga gcaggtcgtg gcaattgcga gcaacggagg
     2701 gggaaagcag gcactcgaaa ccgtccagag gttgctgcct gtgctgtgcc aagcgcacgg
     2761 cctcacccca gagcaggtcg tggcaattgc gagcaacgga gggggaaagc aggcactcga
     2821 aaccgtccag aggttgctgc ctgtgctgtg ccaagcgcac ggacttacgc cagagcaggt
     2881 cgtggcaatt gcgagcaacg gagggggaaa gcaggcactc gaaaccgtcc agaggttgct
     2941 gcctgtgctg tgccaagcgc acggactaac cccagagcag gtcgtggcaa ttgcgagcaa
     3001 ccacggggga aagcaggcac tcgaaaccgt ccagaggttg ctgcctgtgc tgtgccaagc
     3061 gcacgggttg accccagagc aggtcgtggc aattgcgagc aacatcgggg gaaagcaggc
     3121 actcgaaacc gtccagaggt tgctgcctgt gctgtgccaa gcgcacggcc tgaccccaga
     3181 gcaggtcgtg gcaattgcga gcaacatcgg gggaaagcag gcactcgaaa ccgtccagag
     3241 gttgctgcct gtgctgtgcc aagcgcacgg actgacacca gagcaggtcg tggcaattgc
     3301 gagcaaccac gggggaaagc aggcactcga aaccgtccag aggttgctgc ctgtgctgtg
     3361 ccaagcgcac ggactcacgc ctgagcaggt agtggctatt gcatccaacc acgggggcag
     3421 acccgcactg gagtcaatcg tggcccagct ttcgaggccg gaccccgcgc tggccgcact
     3481 cactaatgat catcttgtag cgctggcctg cctcggcgga cgacccgcct tggatgcggt
     3541 gaagaagggg ctcccgcacg cgcctgcatt gattaagcgg accaacagaa ggattcccga
     3601 gaggacatca catcgagtgg caggcctgca gggaagtgga agtatggtga gcaagggcga
     3661 ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca
     3721 caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa
     3781 gctgatctgc accaccggca agctgcccgt gccctggccc accctcgtga ccaccctggg
     3841 ctacggcctg cagtgcttcg cccgctaccc cgaccacatg aagcagcacg acttcttcaa
     3901 gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa
     3961 ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct
     4021 gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta
     4081 caacagccac aacgtctata tcaccgccga caagcagaag aacggcatca aggccaactt
     4141 caagatccgc cacaacatcg aggacggcgg cgtgcagctc gccgaccact accagcagaa
     4201 cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga gctaccagtc
     4261 caagctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac
     4321 cgccgccggg atcactctcg gcatggacga gctgtacaag tagtctagag ggcccgttta
     4381 aacccgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc
     4441 ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga
     4501 ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca
     4561 ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc
     4621 tatggcttct gaggcggaaa gaaccagctg gggctctagg gggtatcccc acgcgccctg
     4681 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
     4741 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
     4801 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
     4861 gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg
     4921 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
     4981 ccaaactgga acaacactca accctatctc ggtctattct tttgatttat aagggatttt
     5041 gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaatta
     5101 attctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga
     5161 agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc
     5221 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc
     5281 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
     5341 tgactaattt tttttattta tgcagaggcc gaggccgcct ctgcctctga gctattccag
     5401 aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc gggagcttgt
     5461 atatccattt tcggatctga tcagcacgtg atgaaaaagc ctgaactcac cgcgacgtct
     5521 gtcgagaagt ttctgatcga aaagttcgac agcgtctccg acctgatgca gctctcggag
     5581 ggcgaagaat ctcgtgcttt cagcttcgat gtaggagggc gtggatatgt cctgcgggta
     5641 aatagctgcg ccgatggttt ctacaaagat cgttatgttt atcggcactt tgcatcggcc
     5701 gcgctcccga ttccggaagt gcttgacatt ggggaattca gcgagagcct gacctattgc
     5761 atctcccgcc gtgcacaggg tgtcacgttg caagacctgc ctgaaaccga actgcccgct
     5821 gttctgcagc cggtcgcgga ggccatggat gcgatcgctg cggccgatct tagccagacg
     5881 agcgggttcg gcccattcgg accgcaagga atcggtcaat acactacatg gcgtgatttc
     5941 atatgcgcga ttgctgatcc ccatgtgtat cactggcaaa ctgtgatgga cgacaccgtc
     6001 agtgcgtccg tcgcgcaggc tctcgatgag ctgatgcttt gggccgagga ctgccccgaa
     6061 gtccggcacc tcgtgcacgc ggatttcggc tccaacaatg tcctgacgga caatggccgc
     6121 ataacagcgg tcattgactg gagcgaggcg atgttcgggg attcccaata cgaggtcgcc
     6181 aacatcttct tctggaggcc gtggttggct tgtatggagc agcagacgcg ctacttcgag
     6241 cggaggcatc cggagcttgc aggatcgccg cggctccggg cgtatatgct ccgcattggt
     6301 cttgaccaac tctatcagag cttggttgac ggcaatttcg atgatgcagc ttgggcgcag
     6361 ggtcgatgcg acgcaatcgt ccgatccgga gccgggactg tcgggcgtac acaaatcgcc
     6421 cgcagaagcg cggccgtctg gaccgatggc tgtgtagaag tactcgccga tagtggaaac
     6481 cgacgcccca gcactcgtcc gagggcaaag gaatagcacg tgctacgaga tttcgattcc
     6541 accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg
     6601 atcctccagc gcggggatct catgctggag ttcttcgccc accccaactt gtttattgca
     6661 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     6721 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctgtata
     6781 ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat
     6841 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
     6901 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
     6961 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     7021 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     7081 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     7141 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     7201 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     7261 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     7321 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     7381 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     7441 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     7501 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     7561 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     7621 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     7681 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     7741 accgctggta gcggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     7801 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     7861 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     7921 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     7981 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     8041 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     8101 gcaatgatac cgcgagaacc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     8161 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     8221 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     8281 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     8341 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     8401 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     8461 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     8521 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     8581 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     8641 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     8701 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     8761 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     8821 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     8881 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     8941 acatttcccc gaaaagtgcc acctgacgtc
//
