LOCUS       sequence-74795-e         997 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses hTTP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 997)
  TITLE     pFRT-TODestFLAGHAhTTP
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 997)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pFRT-TODestFLAGHAhTTP
FEATURES             Location/Qualifiers
     source          1..997
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    18..36
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    39..57
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     67..91
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             191..214
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             215..241
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    257..281
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ctatataagc agagctctcc ctatcagtga tagagatctc cctatcagtg atagagatcg
       61 tcgacgagct cgtttagtga accgtcagat cgcctggaga cgccatccac gctgttttga
      121 cctccataga agacaccggg accgatccag cctccggact ctagcgttta aacttaagct
      181 tggtaccatg gactacaagg acgacgatga caagtaccct tatgacgtgc ccgattacgc
      241 tttgaaccag atatcaacaa gtttgtacaa aaaagcaggc tccgcggccg cccccttcac
      301 catggatctg actgccatct acgagagcct cctgtcgctg agccctgacg tgcccgtgcc
      361 atccgaccat ggagggactg agtccagccc aggctggggc tcctcgggac cctggagcct
      421 gagcccctcc gactccagcc cgtctggggt cacctcccgc ctgcctggcc gctccaccag
      481 cctagtggag ggccgcagct gtggctgggt gcccccaccc cctggcttcg caccgctggc
      541 tccccgcctg ggccctgagc tgtcaccctc acccacttcg cccactgcaa cctccaccac
      601 cccctcgcgc tacaagactg agctatgtcg gaccttctca gagagtgggc gctgccgcta
      661 cggggccaag tgccagtttg cccatggcct gggcgagctg cgccaggcca atcgccaccc
      721 caaatacaag acggaactct gtcacaagtt ctacctccag ggccgctgcc cctacggctc
      781 tcgctgccac ttcatccaca accctagcga agacctggcg gccccgggcc accctcctgt
      841 gcttcgccag agcatcagct tctccggcct gccctctggc cgccggacct caccaccacc
      901 accaggcctg gccggccctt ccctgtcctc cagctccttc tcgccctcca gctccccacc
      961 accacctggg gaccttccac tgtcaccctc tgccttc
//
