LOCUS       sequence-74808-e        9017 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of BTK-mCherry in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9017)
  AUTHORS   Strijbis K, Tafesse FG, Fairn GD, Witte MD, Dougan SK, Watson N, 
            Spooner E, Esteban A, Vyas VK, Fink GR, Grinstein S, Ploegh HL
  TITLE     Bruton's Tyrosine Kinase (BTK) and Vav1 contribute to 
            Dectin1-dependent phagocytosis of Candida albicans in macrophages.
  JOURNAL   PLoS Pathog. 2013 Jun;9(6):e1003446. doi: 
            10.1371/journal.ppat.1003446. Epub 2013 Jun 27.
  PUBMED    23825946
REFERENCE   2  (bases 1 to 9017)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Pathog."; date: "2013-06"; volume: "9(6):e1003446. doi"; pages: " 
            10.1371/journal.ppat.1003446. Epub 2013 Jun 27"
COMMENT     Sequence Label: pMSCV-BTK-mCherry
FEATURES             Location/Qualifiers
     source          1..9017
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    579..920
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     CDS             987..1403
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1333..1355
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1371..1387
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     regulatory      1422..1431
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3441..4148
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(3583..3601)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3851..3870
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     4045..4068
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     promoter        4160..4659
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4195..4218)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     4592..4611
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             4680..5279
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4680..4699)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5176..5196
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             5379..5893
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem 
                     cell virus"
     primer_bind     complement(6062..6078)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6062..6078)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6075..6097)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6086..6102
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6110..6116,6117..6134,6135..6140))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6155..6176
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6293..6310)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6464..7052)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6544..6563)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7223..8014,8015..8083))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(8084..8188)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8256..8274
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8312..8334)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8434..8453
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8647..8669
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8797..8816
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     LTR             join(9016..9017,1..515)
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
ORIGIN
        1 tgaaagaccc cacctgtagg tttggcaagc tagcttaagt aacgccattt tgcaaggcat
       61 ggaaaataca taactgagaa tagagaagtt cagatcaagg ttaggaacag agagacagca
      121 gaatatgggc caaacaggat atctgtggta agcagttcct gccccggctc agggccaaga
      181 acagatggtc cccagatgcg gtcccgccct cagcagtttc tagagaacca tcagatgttt
      241 ccagggtgcc ccaaggacct gaaatgaccc tgtgccttat ttgaactaac caatcagttc
      301 gcttctcgct tctgttcgcg cgcttctgct ccccgagctc aataaaagag cccacaaccc
      361 ctcactcggc gcgccagtcc tccgatagac tgcgtcgccc gggtacccgt attcccaata
      421 aagcctcttg ctgtttgcat ccgaatcgtg gactcgctga tccttgggag ggtctcctca
      481 gattgattga ctgcccacct cgggggtctt tcatttggag gttccaccga gatttggaga
      541 cccctgccca gggaccaccg acccccccgc cgggaggtaa gctggccagc ggtcgtttcg
      601 tgtctgtctc tgtctttgtg cgtgtttgtg ccggcatcta atgtttgcgc ctgcgtctgt
      661 actagttagc taactagctc tgtatctggc ggacccgtgg tggaactgac gagttctgaa
      721 cacccggccg caaccctggg agacgtccca gggactttgg gggccgtttt tgtggcccga
      781 cctgaggaag ggagtcgatg tggaatccga ccccgtcagg atatgtggtt ctggtaggag
      841 acgagaacct aaaacagttc ccgcctccgt ctgaattttt gctttcggtt tggaaccgaa
      901 gccgcgcgtc ttgtctgctg cagcgctgca gcatcgttct gtgttgtctc tgtctgactg
      961 tgtttctgta tttgtctgaa aattagggcc agactgttac cactccctta agtttgacct
     1021 taggtcactg gaaagatgtc gagcggatcg ctcacaacca gtcggtagat gtcaagaaga
     1081 gacgttgggt taccttctgc tctgcagaat ggccaacctt taacgtcgga tggccgcgag
     1141 acggcacctt taaccgagac ctcatcaccc aggttaagat caaggtcttt tcacctggcc
     1201 cgcatggaca cccagaccag gtcccctaca tcgtgacctg ggaagccttg gcttttgacc
     1261 cccctccctg ggtcaagccc tttgtacacc ctaagcctcc gcctcctctt cctccatccg
     1321 ccccgtctct cccccttgaa cctcctcgtt cgaccccgcc tcgatcctcc ctttatccag
     1381 ccctcactcc ttctctaggc gccggaatta gatctctcga ggccaccatg gctgcagtga
     1441 tactggagag catctttctg aagcgctccc agcagaaaaa gaaaacatca cctttaaact
     1501 tcaagaagcg cctgtttctc ttgactgtac acaaactttc atactatgaa tatgactttg
     1561 aacgtgggag aagaggcagt aagaaaggtt caatagatgt tgagaagatc acctgtgttg
     1621 aaacagtaat tcctgaaaaa aatcccccac cagaaagaca gattccgagg agaggtgagg
     1681 agtctagtga aatggaacag atttcaatca ttgaaaggtt cccgtaccca ttccaggttg
     1741 tatatgatga aggacctctc tatgttttct ccccaactga agagctgaga aagcgctgga
     1801 ttcaccagct caaaaatgta atccggtaca atagtgacct ggtacagaaa taccatcctt
     1861 gcttctggat tgatggacag tatctctgct gctctcagac agccaagaat gctatgggct
     1921 gccaaatttt ggagaacagg aatggaagct taaaacctgg gagttctcat cgaaaaacga
     1981 aaaagcctct tccccctacc ccagaggaag atcagatctt gaaaaaaccg cttcccccgg
     2041 agccaacagc agcaccaatc tccacaaccg agctgaaaaa ggtcgtggcc ctttatgatt
     2101 acatgccaat gaacgcaaat gacttacaat tgcgaaaggg cgaggagtat tttatcctgg
     2161 aggagagcaa cctaccgtgg tggcgagcac gagataaaaa tgggcaggaa ggctacatcc
     2221 caagtaacta tatcactgaa gctgaggact ccatagagat gtatgagtgg tattccaagc
     2281 acatgactcg aagtcaagct gagcaactgc taaagcaaga ggggaaagaa ggaggtttca
     2341 ttgtcagaga ctccagcaaa gctggaaaat acaccgtgtc tgtgtttgct aaatctactg
     2401 gggagcctca aggggtgatc cgccattacg ttgtgtgttc cacgccacag agccagtatt
     2461 acctggctga gaaacacctc ttcagcacca tccctgagct cattaactac catcaacaca
     2521 actctgcagg cctcatatcc aggctgaaat atcctgtgtc taaacaaaac aaaaacgcgc
     2581 cttctactgc aggcctgggc tatggatcat gggaaattga tccaaaggac ctcaccttct
     2641 tgaaggagct tgggactgga caattcggtg tcgtgaaata tgggaagtgg aggggccaat
     2701 atgatgtggc catcaagatg atcagagaag gttccatgtc ggaggatgaa ttcattgaag
     2761 aagccaaagt catgatgaat ctttcccatg agaagctggt gcagttgtat ggcgtctgca
     2821 ccaaacaacg ccccatcttc atcatcaccg agtacatggc taatggctgc ctcttgaact
     2881 acctgaggga gatgcggcac cgcttccaga cacagcagct gcttgagatg tgcaaagatg
     2941 tctgtgaagc aatggaatac ttggagtcga agcagttcct tcacagagac ctggcagctc
     3001 gaaactgttt ggtaaacgat caaggagttg tgaaagtatc tgactttggc ctgtctaggt
     3061 atgtccttga tgatgagtac accagctctg taggctccaa gtttccagtc cggtggtctc
     3121 caccagaagt gcttatgtat agcaagttca gcagcaaatc tgacatctgg gcttttgggg
     3181 ttttaatgtg ggagatctac tccctgggga agatgccgta tgagagattt actaacagtg
     3241 agacagcaga acacattgct caaggcttac gtctctacag gcctcatctg gcatcagaga
     3301 gggtatatac catcatgtac agctgctggc acgagaaagc agatgaacgt cctagtttca
     3361 aaattctctt gagtaacatt ctagatgtga tggatgaaga atcccctagg ggaggaggag
     3421 gatcaggagg aggaggatca gtgagcaagg gcgaggagga taacatggcc atcatcaagg
     3481 agttcatgcg cttcaaggtg cacatggagg gctccgtgaa cggccacgag ttcgagatcg
     3541 agggcgaggg cgagggccgc ccctacgagg gcacccagac cgccaagctg aaggtgacca
     3601 agggcggccc cctgcccttc gcctgggaca tcctgtcccc tcagttcatg tacggctcca
     3661 aggcctacgt gaagcacccc gccgacatcc ccgactactt gaagctgtcc ttccccgagg
     3721 gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc gtgacccagg
     3781 actcctccct gcaggacggc gagttcatct acaaggtgaa gctgcgcggc accaacttcc
     3841 cctccgacgg ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc tccgagcgga
     3901 tgtaccccga ggacggcgcc ctgaagggcg agatcaagca gaggctgaag ctgaaggacg
     3961 gcggccacta cgacgccgag gtcaagacca cctacaaggc caagaagccc gtgcagctgc
     4021 ccggcgccta caacgtcaac atcaagctgg acatcacctc ccacaacgag gactacacca
     4081 tcgtggaaca gtacgagcgc gccgagggcc gccactccac cggcggcatg gacgagctgt
     4141 acaagtaggg aattctaccg ggtaggggag gcgcttttcc caaggcagtc tggagcatgc
     4201 gctttagcag ccccgctggg cacttggcgc tacacaagtg gcctctggcc tcgcacacat
     4261 tccacatcca ccggtaggcg ccaaccggct ccgttctttg gtggcccctt cgcgccacct
     4321 tctactcctc ccctagtcag gaagttcccc cccgccccgc agctcgcgtc gtgcaggacg
     4381 tgacaaatgg aagtagcacg tctcactagt ctcgtgcaga tggacagcac cgctgagcaa
     4441 tggaagcggg taggcctttg gggcagcggc caatagcagc tttgctcctt cgctttctgg
     4501 gctcagaggc tgggaagggg tgggtccggg ggcgggctca ggggcgggct caggggcggg
     4561 gcgggcgccc gaaggtcctc cggaggcccg gcattctgca cgcttcaaaa gcgcacgtct
     4621 gccgcgctgt tctcctcttc ctcatctccg ggcctttcga cctgcagccc aagcttacca
     4681 tgaccgagta caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac
     4741 gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc gatccggacc
     4801 gccacatcga gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc gggctcgaca
     4861 tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc acgccggaga
     4921 gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg catggccgag ttgagcggtt
     4981 cccggctggc cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg cccaaggagc
     5041 ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca ccagggcaag ggtctgggca
     5101 gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg
     5161 agacctccgc gccccgcaac ctccccttct acgagcggct cggcttcacc gtcaccgccg
     5221 acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc ggtgcctgac
     5281 gcccgcccca cgacccgcag cgcccgaccg aaaggagcgc acgaccccat gcatcgataa
     5341 aataaaagat tttatttagt ctccagaaaa aggggggaat gaaagacccc acctgtaggt
     5401 ttggcaagct agcttaagta acgccatttt gcaaggcatg gaaaatacat aactgagaat
     5461 agagaagttc agatcaaggt taggaacaga gagacagcag aatatgggcc aaacaggata
     5521 tctgtggtaa gcagttcctg ccccggctca gggccaagaa cagatggtcc ccagatgcgg
     5581 tcccgccctc agcagtttct agagaaccat cagatgtttc cagggtgccc caaggacctg
     5641 aaatgaccct gtgccttatt tgaactaacc aatcagttcg cttctcgctt ctgttcgcgc
     5701 gcttctgctc cccgagctca ataaaagagc ccacaacccc tcactcggcg cgccagtcct
     5761 ccgatagact gcgtcgcccg ggtacccgtg tatccaataa accctcttgc agttgcatcc
     5821 gacttgtggt ctcgctgttc cttgggaggg tctcctctga gtgattgact acccgtcagc
     5881 gggggtcttt catgggtaac agtttcttga agttggagaa caacattctg agggtaggag
     5941 tcgaatatta agtaatcctg actcaattag ccactgtttt gaatccacat actccaatac
     6001 tcctgaaata gttcattatg gacagcgcag aagagctggg gagaattaat tcgtaatcat
     6061 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
     6121 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
     6181 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
     6241 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     6301 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     6361 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     6421 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     6481 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     6541 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     6601 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     6661 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     6721 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     6781 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     6841 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     6901 gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     6961 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     7021 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     7081 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     7141 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     7201 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     7261 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     7321 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     7381 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     7441 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     7501 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
     7561 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     7621 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     7681 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     7741 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     7801 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
     7861 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     7921 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     7981 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     8041 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     8101 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     8161 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct
     8221 aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc
     8281 gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg
     8341 tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg
     8401 gtgttggcgg gtgtcggggc tggcttaact atgcggcatc agagcagatt gtactgagag
     8461 tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc
     8521 gccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc
     8581 tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag
     8641 ggttttccca gtcacgacgt tgtaaaacga cggcgcaagg aatggtgcat gcaaggagat
     8701 ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct
     8761 catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc
     8821 agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggcgatt
     8881 agtccaattt gttaaagaca ggatatcagt ggtccaggct ctagttttga ctcaacaata
     8941 tcaccagctg aagcctatag agtacgagcc atagataaaa taaaagattt tatttagtct
     9001 ccagaaaaag gggggaa
//
