LOCUS       sequence-74809-e        9580 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of mCherry-Vav1 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9580)
  AUTHORS   Strijbis K, Tafesse FG, Fairn GD, Witte MD, Dougan SK, Watson N, 
            Spooner E, Esteban A, Vyas VK, Fink GR, Grinstein S, Ploegh HL
  TITLE     Bruton's Tyrosine Kinase (BTK) and Vav1 contribute to 
            Dectin1-dependent phagocytosis of Candida albicans in macrophages.
  JOURNAL   PLoS Pathog. 2013 Jun;9(6):e1003446. doi: 
            10.1371/journal.ppat.1003446. Epub 2013 Jun 27.
  PUBMED    23825946
REFERENCE   2  (bases 1 to 9580)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Pathog."; date: "2013-06"; volume: "9(6):e1003446. doi"; pages: " 
            10.1371/journal.ppat.1003446. Epub 2013 Jun 27"
COMMENT     Sequence Label: pMSCV-mCherry-Vav1
FEATURES             Location/Qualifiers
     source          1..9580
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    579..920
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     CDS             987..1403
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1333..1355
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1371..1387
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     regulatory      1416..1425
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1422..2129
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1567..1585)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1835..1854
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     2029..2052
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     promoter        4723..5222
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4758..4781)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     5155..5174
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             5243..5842
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5243..5262)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5739..5759
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             5942..6456
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem 
                     cell virus"
     primer_bind     complement(6625..6641)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6625..6641)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6638..6660)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6649..6665
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6673..6679,6680..6697,6698..6703))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6718..6739
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6856..6873)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7027..7615)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7107..7126)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7786..8577,8578..8646))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8409..8428
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8647..8751)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8819..8837
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8875..8897)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8997..9016
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9210..9232
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9360..9379
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     LTR             join(9579..9580,1..515)
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
ORIGIN
        1 tgaaagaccc cacctgtagg tttggcaagc tagcttaagt aacgccattt tgcaaggcat
       61 ggaaaataca taactgagaa tagagaagtt cagatcaagg ttaggaacag agagacagca
      121 gaatatgggc caaacaggat atctgtggta agcagttcct gccccggctc agggccaaga
      181 acagatggtc cccagatgcg gtcccgccct cagcagtttc tagagaacca tcagatgttt
      241 ccagggtgcc ccaaggacct gaaatgaccc tgtgccttat ttgaactaac caatcagttc
      301 gcttctcgct tctgttcgcg cgcttctgct ccccgagctc aataaaagag cccacaaccc
      361 ctcactcggc gcgccagtcc tccgatagac tgcgtcgccc gggtacccgt attcccaata
      421 aagcctcttg ctgtttgcat ccgaatcgtg gactcgctga tccttgggag ggtctcctca
      481 gattgattga ctgcccacct cgggggtctt tcatttggag gttccaccga gatttggaga
      541 cccctgccca gggaccaccg acccccccgc cgggaggtaa gctggccagc ggtcgtttcg
      601 tgtctgtctc tgtctttgtg cgtgtttgtg ccggcatcta atgtttgcgc ctgcgtctgt
      661 actagttagc taactagctc tgtatctggc ggacccgtgg tggaactgac gagttctgaa
      721 cacccggccg caaccctggg agacgtccca gggactttgg gggccgtttt tgtggcccga
      781 cctgaggaag ggagtcgatg tggaatccga ccccgtcagg atatgtggtt ctggtaggag
      841 acgagaacct aaaacagttc ccgcctccgt ctgaattttt gctttcggtt tggaaccgaa
      901 gccgcgcgtc ttgtctgctg cagcgctgca gcatcgttct gtgttgtctc tgtctgactg
      961 tgtttctgta tttgtctgaa aattagggcc agactgttac cactccctta agtttgacct
     1021 taggtcactg gaaagatgtc gagcggatcg ctcacaacca gtcggtagat gtcaagaaga
     1081 gacgttgggt taccttctgc tctgcagaat ggccaacctt taacgtcgga tggccgcgag
     1141 acggcacctt taaccgagac ctcatcaccc aggttaagat caaggtcttt tcacctggcc
     1201 cgcatggaca cccagaccag gtcccctaca tcgtgacctg ggaagccttg gcttttgacc
     1261 cccctccctg ggtcaagccc tttgtacacc ctaagcctcc gcctcctctt cctccatccg
     1321 ccccgtctct cccccttgaa cctcctcgtt cgaccccgcc tcgatcctcc ctttatccag
     1381 ccctcactcc ttctctaggc gccggaatta gatctgccac catggtgagc aagggcgagg
     1441 aggataacat ggccatcatc aaggagttca tgcgcttcaa ggtgcacatg gagggctccg
     1501 tgaacggcca cgagttcgag atcgagggcg agggcgaggg ccgcccctac gagggcaccc
     1561 agaccgccaa gctgaaggtg accaagggcg gccccctgcc cttcgcctgg gacatcctgt
     1621 cccctcagtt catgtacggc tccaaggcct acgtgaagca ccccgccgac atccccgact
     1681 acttgaagct gtccttcccc gagggcttca agtgggagcg cgtgatgaac ttcgaggacg
     1741 gcggcgtggt gaccgtgacc caggactcct ccctgcagga cggcgagttc atctacaagg
     1801 tgaagctgcg cggcaccaac ttcccctccg acggccccgt aatgcagaag aagaccatgg
     1861 gctgggaggc ctcctccgag cggatgtacc ccgaggacgg cgccctgaag ggcgagatca
     1921 agcagaggct gaagctgaag gacggcggcc actacgacgc cgaggtcaag accacctaca
     1981 aggccaagaa gcccgtgcag ctgcccggcg cctacaacgt caacatcaag ctggacatca
     2041 cctcccacaa cgaggactac accatcgtgg aacagtacga gcgcgccgag ggccgccact
     2101 ccaccggcgg catggacgag ctgtacaagg gaggaggagg atcaggagga ggaggatcac
     2161 ctagggagct ctggcgacag tgcacccact ggctgatcca gtgtcgggtg ctgcctccca
     2221 gccaccgtgt gacctgggag ggggcccagg tgtgtgagct ggcacaggca ctgcgggacg
     2281 gtgtcctctt gtgccaattg cttaacaacc tgcttcccca ggccattaat cttcgcgagg
     2341 ttaacttgcg gccccagatg tcccagttcc tttgtcttaa gaacattcga accttcctgt
     2401 ctacttgctg tgagaagttc ggcctcaagc gcagtgaact ctttgaggct tttgacctct
     2461 tcgatgtgca ggactttgga aaggtcatct acaccctgtc tgctctgtca tggacaccca
     2521 ttgcccagaa caaaggaatc atgcccttcc caacagagga cagcgctctg aacgacgaag
     2581 atatttacag tggcctttca gaccagattg atgacaccgc agaggaagac gaggaccttt
     2641 atgactgcgt ggaaaatgag gaggcagagg gggacgagat ctacgaggac ctaatgcgct
     2701 tggagtcggt gcctacgcca cccaagatga cagagtatga taagcgctgc tgctgcctgc
     2761 gggagatcca gcagacggag gagaagtata cagacacact gggctccatc cagcagcact
     2821 tcatgaagcc tctgcagcga ttccttaagc ctcaagacat ggagaccatc tttgtcaaca
     2881 ttgaggagct gttctctgtg catacccact tcttaaagga actgaaggat gccctggctg
     2941 gcccgggagc aacaacactg tatcaggtct tcatcaagta caaggagagg ttcctggttt
     3001 atggccgtta ttgcagtcag gtggagtcag ccagcaagca cttggatcaa gtggccacag
     3061 cacgggagga tgtgcagatg aagctggagg aatgttctca aagagctaac aatggccgat
     3121 tcaccctacg ggatctgctg atggtaccta tgcagcgggt gctgaagtac cacctccttc
     3181 tccaggagct agtgaaacac acacaggatg ctacagagaa ggagaacctg cggttggccc
     3241 tggacgccat gagggacctg gcacagtgcg tgaacgaggt caagagggac aatgaaaccc
     3301 tacggcagat cacaaacttt cagctgtcca ttgagaacct ggaccagtct ctggctaact
     3361 atggccggcc caagattgac ggtgagctca agattacctc agtggagcgt cgctcaaaga
     3421 cagacaggta tgccttcctg ctggacaaag cactgctcat ctgtaaacgc cgcggggact
     3481 cttacgacct caaagcctcg gtgaacttgc acagcttcca agttcgagat gactcctccg
     3541 gggagcgaga caacaagaag tggagccata tgttccttct gattgaggat caaggcgccc
     3601 agggctatga gctgttcttc aagactcggg agctgaagaa gaagtggatg gaacagttcg
     3661 aaatggccat ctccaacatt tacccagaga atgctacagc caatgggcat gattttcaga
     3721 tgttctcctt tgaggagacc acttcctgca aggcctgcca gatgttactc agaggcacat
     3781 tctaccaggg atatcgctgt tacaggtgcc gggcacctgc acacaaggag tgtctgggga
     3841 gagtgcctcc ctgtggtcgc catgggcaag atttcgcagg aaccatgaag aaggacaagc
     3901 tccatcgaag ggcccaggac aagaaaagga atgaattggg tctgcctaag atggaagtgt
     3961 ttcaggaata ctatgggatc cctcctccac ctggagcctt tgggccattt ttacggctca
     4021 accctgggga cattgtggag ctcactaagg cagaggctga gcacaactgg tgggagggaa
     4081 ggaatactgc tacaaatgaa gtcggctggt ttccctgtaa cagagtgcat ccctatgtcc
     4141 acggccctcc tcaggacctg tctgtgcatc tctggtatgc gggccctatg gaacgagcag
     4201 gcgctgaggg catcctcacc aaccgttctg atgggaccta tctggtgcgg cagagggtga
     4261 aagatacagc ggagttcgcc atcagcatta agtataacgt ggaggtcaag catattaaaa
     4321 tcatgacgtc agaggggttg taccggatca cagagaagaa ggctttccgg ggccttctgg
     4381 aactggtaga gttttatcag cagaattccc tcaaagattg cttcaagtcg ttggacacca
     4441 ccttgcagtt tccttataag gaacctgaga ggagagccat cagcaagcca ccagctggaa
     4501 gcaccaagta ttttggcact gccaaagccc gctacgactt ctgtgcccgg gacaggtcgg
     4561 aactgtccct taaggagggt gatatcatca agatcctcaa taagaaggga cagcaaggct
     4621 ggtggcgtgg ggagatctac ggccggatcg gctggttccc ttctaactat gtggaggaag
     4681 actattccga atattgctga ctcgaggtta acgaattcta ccgggtaggg gaggcgcttt
     4741 tcccaaggca gtctggagca tgcgctttag cagccccgct gggcacttgg cgctacacaa
     4801 gtggcctctg gcctcgcaca cattccacat ccaccggtag gcgccaaccg gctccgttct
     4861 ttggtggccc cttcgcgcca ccttctactc ctcccctagt caggaagttc ccccccgccc
     4921 cgcagctcgc gtcgtgcagg acgtgacaaa tggaagtagc acgtctcact agtctcgtgc
     4981 agatggacag caccgctgag caatggaagc gggtaggcct ttggggcagc ggccaatagc
     5041 agctttgctc cttcgctttc tgggctcaga ggctgggaag gggtgggtcc gggggcgggc
     5101 tcaggggcgg gctcaggggc ggggcgggcg cccgaaggtc ctccggaggc ccggcattct
     5161 gcacgcttca aaagcgcacg tctgccgcgc tgttctcctc ttcctcatct ccgggccttt
     5221 cgacctgcag cccaagctta ccatgaccga gtacaagccc acggtgcgcc tcgccacccg
     5281 cgacgacgtc cccagggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac
     5341 gcgccacacc gtcgatccgg accgccacat cgagcgggtc accgagctgc aagaactctt
     5401 cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt
     5461 ggcggtctgg accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc
     5521 gcgcatggcc gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct
     5581 ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga
     5641 ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg
     5701 cgccggggtg cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg
     5761 gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat
     5821 gacccgcaag cccggtgcct gacgcccgcc ccacgacccg cagcgcccga ccgaaaggag
     5881 cgcacgaccc catgcatcga taaaataaaa gattttattt agtctccaga aaaagggggg
     5941 aatgaaagac cccacctgta ggtttggcaa gctagcttaa gtaacgccat tttgcaaggc
     6001 atggaaaata cataactgag aatagagaag ttcagatcaa ggttaggaac agagagacag
     6061 cagaatatgg gccaaacagg atatctgtgg taagcagttc ctgccccggc tcagggccaa
     6121 gaacagatgg tccccagatg cggtcccgcc ctcagcagtt tctagagaac catcagatgt
     6181 ttccagggtg ccccaaggac ctgaaatgac cctgtgcctt atttgaacta accaatcagt
     6241 tcgcttctcg cttctgttcg cgcgcttctg ctccccgagc tcaataaaag agcccacaac
     6301 ccctcactcg gcgcgccagt cctccgatag actgcgtcgc ccgggtaccc gtgtatccaa
     6361 taaaccctct tgcagttgca tccgacttgt ggtctcgctg ttccttggga gggtctcctc
     6421 tgagtgattg actacccgtc agcgggggtc tttcatgggt aacagtttct tgaagttgga
     6481 gaacaacatt ctgagggtag gagtcgaata ttaagtaatc ctgactcaat tagccactgt
     6541 tttgaatcca catactccaa tactcctgaa atagttcatt atggacagcg cagaagagct
     6601 ggggagaatt aattcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct
     6661 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
     6721 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
     6781 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
     6841 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     6901 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     6961 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     7021 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     7081 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     7141 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     7201 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     7261 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     7321 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     7381 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     7441 gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc
     7501 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     7561 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     7621 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     7681 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     7741 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     7801 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     7861 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     7921 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     7981 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     8041 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     8101 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     8161 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     8221 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     8281 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     8341 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     8401 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     8461 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     8521 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     8581 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     8641 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     8701 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     8761 ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa
     8821 taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg
     8881 acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca
     8941 agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta actatgcggc
     9001 atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt
     9061 aaggagaaaa taccgcatca ggcgccattc gccattcagg ctgcgcaact gttgggaagg
     9121 gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag
     9181 gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggcgca
     9241 aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc
     9301 atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg
     9361 gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg
     9421 atgcgtccgg cgtagaggcg attagtccaa tttgttaaag acaggatatc agtggtccag
     9481 gctctagttt tgactcaaca atatcaccag ctgaagccta tagagtacga gccatagata
     9541 aaataaaaga ttttatttag tctccagaaa aaggggggaa
//
