LOCUS       sequence-74810-e        8932 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of mCherry-Syk in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8932)
  AUTHORS   Strijbis K, Tafesse FG, Fairn GD, Witte MD, Dougan SK, Watson N, 
            Spooner E, Esteban A, Vyas VK, Fink GR, Grinstein S, Ploegh HL
  TITLE     Bruton's Tyrosine Kinase (BTK) and Vav1 contribute to 
            Dectin1-dependent phagocytosis of Candida albicans in macrophages.
  JOURNAL   PLoS Pathog. 2013 Jun;9(6):e1003446. doi: 
            10.1371/journal.ppat.1003446. Epub 2013 Jun 27.
  PUBMED    23825946
REFERENCE   2  (bases 1 to 8932)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS 
            Pathog."; date: "2013-06"; volume: "9(6):e1003446. doi"; pages: " 
            10.1371/journal.ppat.1003446. Epub 2013 Jun 27"
COMMENT     Sequence Label: pMSCV-mCherry-Syk
FEATURES             Location/Qualifiers
     source          1..8932
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    579..920
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     CDS             987..1403
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1333..1355
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1371..1387
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     regulatory      1416..1425
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1422..2129
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1567..1585)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1835..1854
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     2029..2052
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     promoter        4075..4574
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4110..4133)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     4507..4526
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             4595..5194
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4595..4614)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5091..5111
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             5294..5808
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem 
                     cell virus"
     primer_bind     complement(5977..5993)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5977..5993)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5990..6012)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6001..6017
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6025..6031,6032..6049,6050..6055))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6070..6091
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6208..6225)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6379..6967)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6459..6478)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7138..7929,7930..7998))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7761..7780
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7999..8103)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8171..8189
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8227..8249)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8349..8368
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8562..8584
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8712..8731
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     LTR             join(8931..8932,1..515)
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
ORIGIN
        1 tgaaagaccc cacctgtagg tttggcaagc tagcttaagt aacgccattt tgcaaggcat
       61 ggaaaataca taactgagaa tagagaagtt cagatcaagg ttaggaacag agagacagca
      121 gaatatgggc caaacaggat atctgtggta agcagttcct gccccggctc agggccaaga
      181 acagatggtc cccagatgcg gtcccgccct cagcagtttc tagagaacca tcagatgttt
      241 ccagggtgcc ccaaggacct gaaatgaccc tgtgccttat ttgaactaac caatcagttc
      301 gcttctcgct tctgttcgcg cgcttctgct ccccgagctc aataaaagag cccacaaccc
      361 ctcactcggc gcgccagtcc tccgatagac tgcgtcgccc gggtacccgt attcccaata
      421 aagcctcttg ctgtttgcat ccgaatcgtg gactcgctga tccttgggag ggtctcctca
      481 gattgattga ctgcccacct cgggggtctt tcatttggag gttccaccga gatttggaga
      541 cccctgccca gggaccaccg acccccccgc cgggaggtaa gctggccagc ggtcgtttcg
      601 tgtctgtctc tgtctttgtg cgtgtttgtg ccggcatcta atgtttgcgc ctgcgtctgt
      661 actagttagc taactagctc tgtatctggc ggacccgtgg tggaactgac gagttctgaa
      721 cacccggccg caaccctggg agacgtccca gggactttgg gggccgtttt tgtggcccga
      781 cctgaggaag ggagtcgatg tggaatccga ccccgtcagg atatgtggtt ctggtaggag
      841 acgagaacct aaaacagttc ccgcctccgt ctgaattttt gctttcggtt tggaaccgaa
      901 gccgcgcgtc ttgtctgctg cagcgctgca gcatcgttct gtgttgtctc tgtctgactg
      961 tgtttctgta tttgtctgaa aattagggcc agactgttac cactccctta agtttgacct
     1021 taggtcactg gaaagatgtc gagcggatcg ctcacaacca gtcggtagat gtcaagaaga
     1081 gacgttgggt taccttctgc tctgcagaat ggccaacctt taacgtcgga tggccgcgag
     1141 acggcacctt taaccgagac ctcatcaccc aggttaagat caaggtcttt tcacctggcc
     1201 cgcatggaca cccagaccag gtcccctaca tcgtgacctg ggaagccttg gcttttgacc
     1261 cccctccctg ggtcaagccc tttgtacacc ctaagcctcc gcctcctctt cctccatccg
     1321 ccccgtctct cccccttgaa cctcctcgtt cgaccccgcc tcgatcctcc ctttatccag
     1381 ccctcactcc ttctctaggc gccggaatta gatctgccac catggtgagc aagggcgagg
     1441 aggataacat ggccatcatc aaggagttca tgcgcttcaa ggtgcacatg gagggctccg
     1501 tgaacggcca cgagttcgag atcgagggcg agggcgaggg ccgcccctac gagggcaccc
     1561 agaccgccaa gctgaaggtg accaagggcg gccccctgcc cttcgcctgg gacatcctgt
     1621 cccctcagtt catgtacggc tccaaggcct acgtgaagca ccccgccgac atccccgact
     1681 acttgaagct gtccttcccc gagggcttca agtgggagcg cgtgatgaac ttcgaggacg
     1741 gcggcgtggt gaccgtgacc caggactcct ccctgcagga cggcgagttc atctacaagg
     1801 tgaagctgcg cggcaccaac ttcccctccg acggccccgt aatgcagaag aagaccatgg
     1861 gctgggaggc ctcctccgag cggatgtacc ccgaggacgg cgccctgaag ggcgagatca
     1921 agcagaggct gaagctgaag gacggcggcc actacgacgc cgaggtcaag accacctaca
     1981 aggccaagaa gcccgtgcag ctgcccggcg cctacaacgt caacatcaag ctggacatca
     2041 cctcccacaa cgaggactac accatcgtgg aacagtacga gcgcgccgag ggccgccact
     2101 ccaccggcgg catggacgag ctgtacaagg gaggaggagg atcaggagga ggaggatcac
     2161 ctagggcggg aagtgctgtg gacagcgcca accacctgac ctactttttt ggcaacatca
     2221 cccgggaaga ggctgaagac tacctggtcc agggaggcat gaccgatggg ctctacctgc
     2281 tacgccagag ccgcaattac ctgggtggtt ttgctttgtc ggtggctcac aacaggaagg
     2341 cacaccacta caccatcgag agggaactta atggcaccta cgccatctcc gggggcaggg
     2401 cccatgccag cccagcagac ctctgccatt accactccca ggaacctgat ggccttatct
     2461 gcctccttaa gaagcccttc aaccggcccc cgggagtaca gcccaagacc ggaccctttg
     2521 aggacctgaa ggagaacctc atcagggaat atgtgaaaca gacctggaac cttcagggcc
     2581 aggctctgga gcaagccatc atcagccaga agccccagct ggagaagctg atcgccacca
     2641 cggcccatga gaagatgccc tggttccatg gcaacatctc cagagatgaa tcagagcaga
     2701 cggtcctcat agggtcaaag accaatggaa aattcctgat cagggccaga gacaacagcg
     2761 gctcctatgc tctgtgcctg ctgcacgaag ggaaagtatt gcactaccgc attgacaggg
     2821 acaagaccgg gaagctctcc attcctgagg ggaagaagtt tgacaccctc tggcagctag
     2881 tggaacatta ctcttacaag ccagatgggc tactaagagt cctcacggta ccatgccaaa
     2941 agattggtgc acagatgggc cacccaggaa gcccaaatgc ccatcccgtg acttggtcac
     3001 cgggtggaat aatctcaagg atcaaatcct actccttccc aaagcctggc cacaaaaagc
     3061 ctgccccacc ccaagggagc cgtccagaga gcactgtgtc cttcaacccc tatgagccaa
     3121 cgggagggcc ctggggccca gacagaggcc ttcagagaga agccctgccc atggacacag
     3181 aggtgtacga gagcccttat gctgaccctg aagagatccg gcccaaagag gtctacctgg
     3241 acaggagcct gctgaccctg gaggacaatg aactgggctc cggtaacttc gggactgtga
     3301 aaaagggata ctaccaaatg aaaaaagttg tgaaaaccgt ggctgtgaaa atcctgaaga
     3361 acgaggccaa cgacccggct ttgaaggacg agctgctggc agaggcgaac gtcatgcagc
     3421 agctggacaa cccctacatt gtgcgcatga tcggaatctg cgaggcggag tcctggatgc
     3481 tggtgatgga gatggcggag ctggggccgc tcaacaagta cctgcagcag aacaggcaca
     3541 ttaaggataa gaacatcata gagctggttc accaggtttc catggggatg aagtatttgg
     3601 aagagagcaa ctttgtgcac agagatctgg ctgcgcggaa cgtgcttctg gtcacacagc
     3661 actatgccaa gatcagcgat ttcggtcttt ccaaagccct gcgtgctgat gaaaactact
     3721 acaaggccca gacccacggg aagtggcccg tgaagtggta cgcccccgaa tgcatcaact
     3781 actacaagtt ctccagtaag agtgacgtct ggagcttcgg agtcctgatg tgggaagcgt
     3841 tctcctatgg gcagaagccc tacagaggga tgaaagggag cgaagtgacc gccatgctgg
     3901 agaaaggaga gcggatgggg tgccctgcag gatgcccgag agagatgtac gacctgatga
     3961 acctgtgctg gacttacgat gtggagaaca ggccaggatt cacagctgtg gaactgaggc
     4021 ttcgcaatta ctactacgac gtggttaact aactcgaggt taacgaattc taccgggtag
     4081 gggaggcgct tttcccaagg cagtctggag catgcgcttt agcagccccg ctgggcactt
     4141 ggcgctacac aagtggcctc tggcctcgca cacattccac atccaccggt aggcgccaac
     4201 cggctccgtt ctttggtggc cccttcgcgc caccttctac tcctccccta gtcaggaagt
     4261 tcccccccgc cccgcagctc gcgtcgtgca ggacgtgaca aatggaagta gcacgtctca
     4321 ctagtctcgt gcagatggac agcaccgctg agcaatggaa gcgggtaggc ctttggggca
     4381 gcggccaata gcagctttgc tccttcgctt tctgggctca gaggctggga aggggtgggt
     4441 ccgggggcgg gctcaggggc gggctcaggg gcggggcggg cgcccgaagg tcctccggag
     4501 gcccggcatt ctgcacgctt caaaagcgca cgtctgccgc gctgttctcc tcttcctcat
     4561 ctccgggcct ttcgacctgc agcccaagct taccatgacc gagtacaagc ccacggtgcg
     4621 cctcgccacc cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg cgttcgccga
     4681 ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct
     4741 gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga
     4801 cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc
     4861 cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat
     4921 ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg
     4981 cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga
     5041 ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc
     5101 cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg
     5161 cacctggtgc atgacccgca agcccggtgc ctgacgcccg ccccacgacc cgcagcgccc
     5221 gaccgaaagg agcgcacgac cccatgcatc gataaaataa aagattttat ttagtctcca
     5281 gaaaaagggg ggaatgaaag accccacctg taggtttggc aagctagctt aagtaacgcc
     5341 attttgcaag gcatggaaaa tacataactg agaatagaga agttcagatc aaggttagga
     5401 acagagagac agcagaatat gggccaaaca ggatatctgt ggtaagcagt tcctgccccg
     5461 gctcagggcc aagaacagat ggtccccaga tgcggtcccg ccctcagcag tttctagaga
     5521 accatcagat gtttccaggg tgccccaagg acctgaaatg accctgtgcc ttatttgaac
     5581 taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc tgctccccga gctcaataaa
     5641 agagcccaca acccctcact cggcgcgcca gtcctccgat agactgcgtc gcccgggtac
     5701 ccgtgtatcc aataaaccct cttgcagttg catccgactt gtggtctcgc tgttccttgg
     5761 gagggtctcc tctgagtgat tgactacccg tcagcggggg tctttcatgg gtaacagttt
     5821 cttgaagttg gagaacaaca ttctgagggt aggagtcgaa tattaagtaa tcctgactca
     5881 attagccact gttttgaatc cacatactcc aatactcctg aaatagttca ttatggacag
     5941 cgcagaagag ctggggagaa ttaattcgta atcatggtca tagctgtttc ctgtgtgaaa
     6001 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
     6061 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
     6121 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     6181 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     6241 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     6301 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     6361 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     6421 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     6481 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     6541 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     6601 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     6661 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     6721 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     6781 gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc
     6841 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     6901 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     6961 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     7021 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     7081 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     7141 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     7201 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     7261 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     7321 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     7381 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     7441 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     7501 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     7561 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     7621 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     7681 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     7741 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     7801 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     7861 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     7921 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     7981 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
     8041 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
     8101 gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt
     8161 aacctataaa aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg
     8221 tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc
     8281 cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct
     8341 taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt gtgaaatacc
     8401 gcacagatgc gtaaggagaa aataccgcat caggcgccat tcgccattca ggctgcgcaa
     8461 ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg
     8521 atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgttgtaa
     8581 aacgacggcg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg
     8641 gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga
     8701 tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg
     8761 atgccggcca cgatgcgtcc ggcgtagagg cgattagtcc aatttgttaa agacaggata
     8821 tcagtggtcc aggctctagt tttgactcaa caatatcacc agctgaagcc tatagagtac
     8881 gagccataga taaaataaaa gattttattt agtctccaga aaaagggggg aa
//
