LOCUS       sequence-74811-e        6071 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Integrating vector with an improved Red-shifted Thermostable Firefly
            Luciferase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6071)
  AUTHORS   Andreu N, Zelmer A, Sampson SL, Ikeh M, Bancroft GJ, Schaible UE, 
            Wiles S, Robertson BD
  TITLE     Rapid in vivo assessment of drug efficacy against Mycobacterium 
            tuberculosis using an improved firefly luciferase.
  JOURNAL   J Antimicrob Chemother. 2013 Sep;68(9):2118-27. doi: 
            10.1093/jac/dkt155. Epub 2013 Apr 30.
  PUBMED    23633686
REFERENCE   2  (bases 1 to 6071)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/jac/dkt155"; journalName: "J Antimicrob Chemother"; date: 
            "2013-09"; volume: "68"; issue: "9"; pages: "2118-27"
COMMENT     Sequence Label: pMV306G13+FFlucRT
FEATURES             Location/Qualifiers
     source          1..6071
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(1896..1915)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1907..2722
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPHAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHNLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1980..1999)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3054..3642
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3543..3562
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      5989..6035
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 gctagagccg tgaacgacag ggcgaacgcc agcccgccga cggcgagggt tccgaccgct
       61 gcaactcccg gtgcaacctt gtcccggtct attctcttca ctgcaccagc tccaatctgg
      121 tgtgaatgcc cctcgtctgt tcgcgcaggc ggggggctct attcgtttgt cagcatcgaa
      181 agtagccaga tcagggatgc gttgcaaccg cgtatgccca ggtcagaaga gtcgcacaag
      241 agttgcagac ccctggaaag aaaaatggcc agagggcgaa aacaccctct gaccagcgga
      301 gcgggcgacg ggaatcgaac ccgcgtagct agtttggaag aatgggtgtc tgccgaccac
      361 atatatgggc cggtcaagat aggtttttac cccctctcgg ctgcatcctc taagtggaaa
      421 gaaattgcag gtcgtagaag cgcgttgaag cctgagagtt gcacaggagt tgcaacccgg
      481 tagccttgtt cacgacgaga ggagacctag ttggcacgtc gcggatgggg atcgctgaag
      541 actcagcgca gcgggaggat ccaagcctca tacgtcaacc cgcaggacgg tgtgaggtac
      601 tacgcgctgc agacctacga caacaagatg gacgccgaag cctggctcgc gggcgagaag
      661 cggctcatcg agatggagac ctggacccct ccacaggacc gggcgaagaa ggcagccgcc
      721 agcgccatca cgctggagga gtacacccgg aagtggctcg tggagcgcga cctcgcagac
      781 ggcaccaggg atctgtacag cgggcacgcg gagcgccgca tctacccggt gctaggtgaa
      841 gtggcggtca cagagatgac gccagctctg gtgcgtgcgt ggtgggccgg gatgggtagg
      901 aagcacccga ctgcccgccg gcatgcctac aacgtcctcc gggcggtgat gaacacagcg
      961 gtcgaggaca agctgatcgc agagaacccg tgccggatcg agcagaaggc agccgatgag
     1021 cgcgacgtag aggcgctgac gcctgaggag ctggacatcg tcgccgctga gatcttcgag
     1081 cactaccgga tcgcggcata catcctggcg tggacgagcc tccggttcgg agagctgatc
     1141 gagcttcgcc gcaaggacat cgtggacgac ggcatgacga tgaagctccg ggtgcgccgt
     1201 ggcgcttccc gcgtggggaa caagatcgtc gttggcaacg ccaagaccgt ccggtcgaag
     1261 cgtcctgtga cggttccgcc tcacgtcgcg gagatgatcc gagcgcacat gaaggaccgt
     1321 acgaagatga acaagggccc cgaggcattc ctggtgacca cgacgcaggg caaccggctg
     1381 tcgaagtccg cgttcaccaa gtcgctgaag cgtggctacg ccaagatcgg tcggccggaa
     1441 ctccgcatcc acgacctccg cgctgtcggc gctacgttcg ccgctcaggc aggtgcgacg
     1501 accaaggagc tgatggcccg tctcggtcac acgactccta ggatggcgat gaagtaccag
     1561 atggcgtctg aggcccgcga cgaggctatc gctgaggcga tgtccaagct ggccaagacc
     1621 tcctgaaacg caaaaagccc ccctcccaag gacactgagt cctaaagagg ggggtttctt
     1681 gtcagtacgc gaagaaccac gcctggccgc gagcgccagc accgccgctc tgtgcggaga
     1741 cctgggcacc agccccgccg ccgccaggag cattgccgtt cccgccagct agcaacaaag
     1801 cgacgttgtg tctcaaaatc tctgatgtta cattgcacaa gataaaaata tatcatcatg
     1861 aacaataaaa ctgtctgctt acataaacag taatacaagg ggtgttatga gccatattca
     1921 acgggaaacg tcttgctcga ggccgcgatt aaattccaac atggatgctg atttatatgg
     1981 gtataaatgg gctcgcgata atgtcgggca atcaggtgcg acaatctatc gcttgtatgg
     2041 gaagccccat gcgccagagt tgtttctgaa acatggcaaa ggtagcgttg ccaatgatgt
     2101 tacagatgag atggtcagac taaactggct gacggaattt atgcctcttc cgaccatcaa
     2161 gcattttatc cgtactcctg atgatgcatg gttactcacc actgcgatcc ccgggaaaac
     2221 agcattccag gtattagaag aatatcctga ttcaggtgaa aatattgttg atgcgctggc
     2281 agtgttcctg cgccggttgc attcgattcc tgtttgtaat tgtcctttta acagcgatcg
     2341 cgtatttcgt ctcgctcagg cgcaatcacg aatgaataac ggtttggttg atgcgagtga
     2401 ttttgatgac gagcgtaatg gctggcctgt tgaacaagtc tggaaagaaa tgcataatct
     2461 tttgccattc tcaccggatt cagtcgtcac tcatggtgat ttctcacttg ataaccttat
     2521 ttttgacgag gggaaattaa taggttgtat tgatgttgga cgagtcggaa tcgcagaccg
     2581 ataccaggat cttgccatcc tatggaactg cctcggtgag ttttctcctt cattacagaa
     2641 acggcttttt caaaaatatg gtattgataa tcctgatatg aataaattgc agtttcattt
     2701 gatgctcgat gagtttttct aatcagaatt ggttaattgg ttgtaacact ggcagagcat
     2761 tacgctgact tgacgggacg gcggctttgt tgaataaatc gaacttttgc tgagttgaag
     2821 gatcagatca cgcatcttcc cgacaacgca gaccgttccg tggcaaagca aaagttcaaa
     2881 atcaccaact ggtccaccta caacaaagct ctcatcaacc gtggctccct cactttctgg
     2941 ctggatgatg gggcgattca ggcctggtat gagtcagcaa caccttcttc acgaggcaga
     3001 cctcactagt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     3061 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     3121 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     3181 gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac
     3241 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     3301 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     3361 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     3421 gaactgagat acctacagcg tgagcattga gaaagcgcca cgcttcccga agggagaaag
     3481 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     3541 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     3601 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     3661 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     3721 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
     3781 cgaacgaccg agcgcaacgc gtgcggccgc gatcgccact agcgccgcgg tcggaatcag
     3841 cgcactggcc gccggagcgg tcagctacct gttgctgcgc cgcttcgctt tctagcaccg
     3901 acgtcccggc gccagcagct acctctcgag caaacgtttt gcgcgcccga aaaataagtc
     3961 gttgcagaac tttcatgaat taggccttgc tgcgcccagg ctccagtagt agaaatggag
     4021 tcacggcagc ccggtgaagc caaggtcgaa ccggaagaga aggttcgtcc tcccgacccg
     4081 ggcacccagc acggcccccg gaacccacgc ggagtcatag ccgcgataat ggcagaagtg
     4141 ttgcgggcct gcgtaattgc gaaattcaga tggtgccgac ggccctttgg gtggggctgc
     4201 agccagaagg gtcgcaaaag cgccgaggcc aacccacgca gcccacaaat gcacgcttgg
     4261 taaccgagaa ttcagaagga gaagtaccga tggaggacgc taagaacatc aagaaaggac
     4321 ccgctccgtt ttatccgctg gaggacggaa cggcaggcga acaactgcac aaggccatga
     4381 agcggtacgc cctcgtgcca ggcaccattg ccttcaccga cgcgcacatt gaggtcaaca
     4441 tcacctacgc tgagtacttc gagatgtcgg tgcgcctggc ggaggctatg aagcgttacg
     4501 gcctgaacac caaccaccgt atcgtggtct gctccgagaa ctcgctgcag tttttcatgc
     4561 cggtgctggg cgcgttgttc attggcgtgg cagttgcacc ggcgaatgac atctacaacg
     4621 aacgggagct gctcaactcg atgaacatca gccaacccac cgttgttttc gtgagcaaga
     4681 aaggcctgca aaagatcctc aacgttcaga agaagctccc gatcatccag aagatcatca
     4741 tcatggactc gaaaacggac taccagggtt tccagagcat gtacacgttc gtgaccagcc
     4801 acctgccccc aggttttaat gagtacgact tcgtcccgga atcgtttgac cgggacaaga
     4861 ccatcgctct gatcatgaac tcgagcggat cgaccggtct gccgaagggc gtggcattgc
     4921 cgcatcgcgc actgtgcgtg cgtttctccc acgcgcgcga cccgatcttc ggaaaccaaa
     4981 tcgctccaga caccgcgatc ttgtcggtcg tgccgttcca tcatggcttt gggatgttca
     5041 cgaccctggg ctacctcatc tgcggcttcc gggtggtgct gatgtaccgg ttcgaggagg
     5101 agctcttttt gcgcagcctg caggactaca aaatccagac cgccctgctg gtgccaacgt
     5161 tgttctcgtt cttggccaaa tcgaccttga tcgacaagta tgacctgagc aacttgcacg
     5221 aaatcgcaag cggaggcgcc ccattgtcga aagaggtcgg cgaggccgtg gcgaagcgat
     5281 tccacctgcc aggcatccgg caaggttacg gcttgaccga aaccacctcg gctatcctca
     5341 tcacgccgaa gggtgatgac aagccgggag ccgtcggtaa ggtggtcccg ttcttcgagg
     5401 ctaaggttgt ggacctcgac acggggaaaa ccctcggcgt gaatcagcgg ggcgagctgt
     5461 gcgtccgcgg gccgatgatc atgtccggct atgtgaacaa cccggaggcg acgaatgccc
     5521 tgatcgataa ggacggttgg ctccactccg gcgacctcgc gtactgggac gaggatgaac
     5581 actttttcat cgtggggcgg ctgaagtccc tgattaaata caaggggtat caggtggcgc
     5641 cagcagaact ggagtcgatc ctgctccagc atccgaacat cttcgatgcc ggcgtcgcgg
     5701 gcctgccgga tgatgacgcg ggagagttgc cggcagccgt ggtcgtcctg gaacacggga
     5761 aaaccatgac ggagaaggag atcgtcgatt atgtcgcctc gcaggtgacc acggcgaaaa
     5821 agctccgtgg cggagtcgtg ttcgtggacg aggtccccaa gggcttgacg ggtaagcgcg
     5881 acgcccggaa aatccgcgag atcttgatta aggccaagaa aggtggcaag tcgaaattgt
     5941 gatctagagt cgacgtagtt aactagcgta cgatcgactg ccaggcatca aataaaacga
     6001 aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtccgg ccatcatggc
     6061 cgcggtgatc a
//
