LOCUS       sequence-74812-e        5028 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Integrase removed to increase stability of the plasmid in 
            mycobacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5028)
  AUTHORS   Andreu N, Zelmer A, Sampson SL, Ikeh M, Bancroft GJ, Schaible UE, 
            Wiles S, Robertson BD
  TITLE     Rapid in vivo assessment of drug efficacy against Mycobacterium 
            tuberculosis using an improved firefly luciferase.
  JOURNAL   J Antimicrob Chemother. 2013 Sep;68(9):2118-27. doi: 
            10.1093/jac/dkt155. Epub 2013 Apr 30.
  PUBMED    23633686
REFERENCE   2  (bases 1 to 5028)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/jac/dkt155"; journalName: "J Antimicrob Chemother"; date: 
            "2013-09"; volume: "68"; issue: "9"; pages: "2118-27"
COMMENT     Sequence Label: pMV306DIG13+FFlucRT
FEATURES             Location/Qualifiers
     source          1..5028
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(853..872)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             864..1679
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPHAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHNLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(937..956)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2011..2599
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2500..2519
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      4946..4992
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 gctagagccg tgaacgacag ggcgaacgcc agcccgccga cggcgagggt tccgaccgct
       61 gcaactcccg gtgcaacctt gtcccggtct attctcttca ctgcaccagc tccaatctgg
      121 tgtgaatgcc cctcgtctgt tcgcgcaggc ggggggctct attcgtttgt cagcatcgaa
      181 agtagccaga tcagggatgc gttgcaaccg cgtatgccca ggtcagaaga gtcgcacaag
      241 agttgcagac ccctggaaag aaaaatggcc agagggcgaa aacaccctct gaccagcgga
      301 gcgggcgacg ggaatcgaac ccgcgtagct agtttggaag aatgggtgtc tgccgaccac
      361 atatatgggc cggtcaagat aggtttttac cccctctcgg ctgcatcctc taagtggaaa
      421 gaaattgcag gtcgtagaag cgcgttgaag cctgagagtt gcacaggagt tgcaacccgg
      481 tagccttgtt cacgacgaga ggagacctag ttggcacgtc gcggatgggg atcgctgaag
      541 actcagcgca gcgggaggat ccaagcctca tacgtcaacc cgcaggacgg tgtgaggtac
      601 tacgcgctgc agacctacga caacaagatg gactcttgtc agtacgcgaa gaaccacgcc
      661 tggccgcgag cgccagcacc gccgctctgt gcggagacct gggcaccagc cccgccgccg
      721 ccaggagcat tgccgttccc gccagctagc aacaaagcga cgttgtgtct caaaatctct
      781 gatgttacat tgcacaagat aaaaatatat catcatgaac aataaaactg tctgcttaca
      841 taaacagtaa tacaaggggt gttatgagcc atattcaacg ggaaacgtct tgctcgaggc
      901 cgcgattaaa ttccaacatg gatgctgatt tatatgggta taaatgggct cgcgataatg
      961 tcgggcaatc aggtgcgaca atctatcgct tgtatgggaa gccccatgcg ccagagttgt
     1021 ttctgaaaca tggcaaaggt agcgttgcca atgatgttac agatgagatg gtcagactaa
     1081 actggctgac ggaatttatg cctcttccga ccatcaagca ttttatccgt actcctgatg
     1141 atgcatggtt actcaccact gcgatccccg ggaaaacagc attccaggta ttagaagaat
     1201 atcctgattc aggtgaaaat attgttgatg cgctggcagt gttcctgcgc cggttgcatt
     1261 cgattcctgt ttgtaattgt ccttttaaca gcgatcgcgt atttcgtctc gctcaggcgc
     1321 aatcacgaat gaataacggt ttggttgatg cgagtgattt tgatgacgag cgtaatggct
     1381 ggcctgttga acaagtctgg aaagaaatgc ataatctttt gccattctca ccggattcag
     1441 tcgtcactca tggtgatttc tcacttgata accttatttt tgacgagggg aaattaatag
     1501 gttgtattga tgttggacga gtcggaatcg cagaccgata ccaggatctt gccatcctat
     1561 ggaactgcct cggtgagttt tctccttcat tacagaaacg gctttttcaa aaatatggta
     1621 ttgataatcc tgatatgaat aaattgcagt ttcatttgat gctcgatgag tttttctaat
     1681 cagaattggt taattggttg taacactggc agagcattac gctgacttga cgggacggcg
     1741 gctttgttga ataaatcgaa cttttgctga gttgaaggat cagatcacgc atcttcccga
     1801 caacgcagac cgttccgtgg caaagcaaaa gttcaaaatc accaactggt ccacctacaa
     1861 caaagctctc atcaaccgtg gctccctcac tttctggctg gatgatgggg cgattcaggc
     1921 ctggtatgag tcagcaacac cttcttcacg aggcagacct cactagttcc actgagcgtc
     1981 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     2041 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     2101 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct
     2161 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     2221 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     2281 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     2341 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     2401 gcattgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     2461 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     2521 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     2581 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     2641 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     2701 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcaacgcgtg
     2761 cggccgcgat cgccactagc gccgcggtcg gaatcagcgc actggccgcc ggagcggtca
     2821 gctacctgtt gctgcgccgc ttcgctttct agcaccgacg tcccggcgcc agcagctacc
     2881 tctcgagcaa acgttttgcg cgcccgaaaa ataagtcgtt gcagaacttt catgaattag
     2941 gccttgctgc gcccaggctc cagtagtaga aatggagtca cggcagcccg gtgaagccaa
     3001 ggtcgaaccg gaagagaagg ttcgtcctcc cgacccgggc acccagcacg gcccccggaa
     3061 cccacgcgga gtcatagccg cgataatggc agaagtgttg cgggcctgcg taattgcgaa
     3121 attcagatgg tgccgacggc cctttgggtg gggctgcagc cagaagggtc gcaaaagcgc
     3181 cgaggccaac ccacgcagcc cacaaatgca cgcttggtaa ccgagaattc agaaggagaa
     3241 gtaccgatgg aggacgctaa gaacatcaag aaaggacccg ctccgtttta tccgctggag
     3301 gacggaacgg caggcgaaca actgcacaag gccatgaagc ggtacgccct cgtgccaggc
     3361 accattgcct tcaccgacgc gcacattgag gtcaacatca cctacgctga gtacttcgag
     3421 atgtcggtgc gcctggcgga ggctatgaag cgttacggcc tgaacaccaa ccaccgtatc
     3481 gtggtctgct ccgagaactc gctgcagttt ttcatgccgg tgctgggcgc gttgttcatt
     3541 ggcgtggcag ttgcaccggc gaatgacatc tacaacgaac gggagctgct caactcgatg
     3601 aacatcagcc aacccaccgt tgttttcgtg agcaagaaag gcctgcaaaa gatcctcaac
     3661 gttcagaaga agctcccgat catccagaag atcatcatca tggactcgaa aacggactac
     3721 cagggtttcc agagcatgta cacgttcgtg accagccacc tgcccccagg ttttaatgag
     3781 tacgacttcg tcccggaatc gtttgaccgg gacaagacca tcgctctgat catgaactcg
     3841 agcggatcga ccggtctgcc gaagggcgtg gcattgccgc atcgcgcact gtgcgtgcgt
     3901 ttctcccacg cgcgcgaccc gatcttcgga aaccaaatcg ctccagacac cgcgatcttg
     3961 tcggtcgtgc cgttccatca tggctttggg atgttcacga ccctgggcta cctcatctgc
     4021 ggcttccggg tggtgctgat gtaccggttc gaggaggagc tctttttgcg cagcctgcag
     4081 gactacaaaa tccagaccgc cctgctggtg ccaacgttgt tctcgttctt ggccaaatcg
     4141 accttgatcg acaagtatga cctgagcaac ttgcacgaaa tcgcaagcgg aggcgcccca
     4201 ttgtcgaaag aggtcggcga ggccgtggcg aagcgattcc acctgccagg catccggcaa
     4261 ggttacggct tgaccgaaac cacctcggct atcctcatca cgccgaaggg tgatgacaag
     4321 ccgggagccg tcggtaaggt ggtcccgttc ttcgaggcta aggttgtgga cctcgacacg
     4381 gggaaaaccc tcggcgtgaa tcagcggggc gagctgtgcg tccgcgggcc gatgatcatg
     4441 tccggctatg tgaacaaccc ggaggcgacg aatgccctga tcgataagga cggttggctc
     4501 cactccggcg acctcgcgta ctgggacgag gatgaacact ttttcatcgt ggggcggctg
     4561 aagtccctga ttaaatacaa ggggtatcag gtggcgccag cagaactgga gtcgatcctg
     4621 ctccagcatc cgaacatctt cgatgccggc gtcgcgggcc tgccggatga tgacgcggga
     4681 gagttgccgg cagccgtggt cgtcctggaa cacgggaaaa ccatgacgga gaaggagatc
     4741 gtcgattatg tcgcctcgca ggtgaccacg gcgaaaaagc tccgtggcgg agtcgtgttc
     4801 gtggacgagg tccccaaggg cttgacgggt aagcgcgacg cccggaaaat ccgcgagatc
     4861 ttgattaagg ccaagaaagg tggcaagtcg aaattgtgat ctagagtcga cgtagttaac
     4921 tagcgtacga tcgactgcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc
     4981 ctttcgtttt atctgttgtt tgtccggcca tcatggccgc ggtgatca
//
