LOCUS       sequence-74814-e        9507 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Integrase removed to increase stability of the plasmid in 
            mycobacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9507)
  AUTHORS   Andreu N, Zelmer A, Sampson SL, Ikeh M, Bancroft GJ, Schaible UE, 
            Wiles S, Robertson BD
  TITLE     Rapid in vivo assessment of drug efficacy against Mycobacterium 
            tuberculosis using an improved firefly luciferase.
  JOURNAL   J Antimicrob Chemother. 2013 Sep;68(9):2118-27. doi: 
            10.1093/jac/dkt155. Epub 2013 Apr 30.
  PUBMED    23633686
REFERENCE   2  (bases 1 to 9507)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/jac/dkt155"; journalName: "J Antimicrob Chemother"; date: 
            "2013-09"; volume: "68"; issue: "9"; pages: "2118-27"
COMMENT     Sequence Label: pMV306DIhsp+LuxG13
FEATURES             Location/Qualifiers
     source          1..9507
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(853..872)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             864..1679
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPHAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHNLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(937..956)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2011..2599
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2500..2519
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             3278..4360
                     /codon_start=1
                     /gene="luxA"
                     /product="LuxA luciferase subunit"
                     /label=LuxA
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MKFGNFLLTYQPPQFSQTEVMKRLVKLGRISEECGFDTVWLLEHH
                     FTEFGLLGNPYVAAAYLLGATKKLNVGTAAIVLPTAHPVRQLEDVNLLDQMSKGRFRFG
                     ICRGLYNKDFRVFGTDMNNSRALAECWYGLIKNGMTEGYMEADNEHIKFHKVKVNPAAY
                     SRGGAPVYVVAESASTTEWAAQFGLPMILSWIINTNEKKAQLELYNEVAQEYGHDIHNI
                     DHCLSYITSVDHDSIKAKEICRKFLGHWYDSYVNATTIFDDSDQTRGYDFNKGQWRDFV
                     LKGHKDTNRRIDYSYEINPVGTPQECIDIIQKDIDATGISNICCGFEANGTVDEIIASM
                     KLFQSDVMPFLKEKQRSLLY"
     CDS             4375..5358
                     /codon_start=1
                     /gene="luxB"
                     /product="LuxB luciferase subunit"
                     /label=LuxB
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MKFGLFFLNFINSTTVQEQSIVRMQEITEYVDKLNFEQILVYENH
                     FSDNGVVGAPLTVSGFLLGLTEKIKIGSLNHIITTHHPVAIAEEACLLDQLSEGRFILG
                     FSDCEKKDEMHFFNRPVEYQQQLFEECYEIINDALTTGYCNPDNDFYSFPKISVNPHAY
                     TPGGPRKYVTATSHHIVEWAAKKGIPLIFKWDDSNDVRYEYAERYKAVADKYDVDLSEI
                     DHQLMILVNYNEDSNKAKQETRAFISDYVLEMHPNENFENKLEEIIAENAVGNYTECIT
                     AAKLAIEKCGAKSVLLSFEPMNDLMSQKNVINIVDDNIKKYHMKYT"
     CDS             5845..7287
                     /codon_start=1
                     /gene="luxC"
                     /product="LuxC fatty acid reductase"
                     /label=LuxC
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MTKKISFIINGQVEIFPESDDLVQSINFGDNSVYLPILNDSHVKN
                     IIDCNGNNELRLHNIVNFLYTVGQRWKNEEYSRRRTYIRDLKKYMGYSEEMAKLEANWI
                     SMILCSKGGLYDVVENELGSRHIMDEWLPQDESYVRAFPKGKSVHLLAGNVPLSGIMSI
                     LRAILTKNQCIIKTSSTDPFTANALALSFIDVDPNHPITRSLSVIYWPHQGDTSLAKEI
                     MRHADVIVAWGGPDAINWAVEHAPSYADVIKFGSKKSLCIIDNPVDLTSAATGAAHDVC
                     FYDQRACFSAQNIYYMGNHYEEFKLALIEKLNLYAHILPNAKKDFDEKAAYSLVQKESL
                     FAGLKVEVDIHQRWMIIESNAGVEFNQPLGRCVYLHHVDNIEQILPYVQKNKTQTISIF
                     PWESSFKYRDALALKGAERIVEAGMNNIFRVGGSHDGMRPLQRLVTYISHERPSNYTAK
                     DVAVEIEQTRFLEEDKFLVFVP"
     CDS             7299..8222
                     /codon_start=1
                     /gene="luxD"
                     /product="LuxD acyltransferase"
                     /label=LuxD
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MENESKYKTIDHVICVEGNKKIHVWETLPEENSPKRKNAIIIASG
                     FARRMDHFAGLAEYLSRNGFHVIRYDSLHHVGLSSGTIDEFTMSIGKQSLLAVVDWLTT
                     RKINNFGMLASSLSARIAYASLSEINASFLITAVGVVNLRYSLERALGFDYLSLPINEL
                     PDNLDFEGHKLGAEVFARDCLDFGWEDLASTINNMMYLDIPFIAFTANNDNWVKQDEVI
                     TLLSNIRSNRCKIYSLLGSSHDLSENLVVLRNFYQSVTKAAIAMDNDHLDIDVDITEPS
                     FEHLTIATVNERRMRIEIENQAISLS"
     CDS             8248..9360
                     /codon_start=1
                     /gene="luxE"
                     /product="LuxE"
                     /label=LuxE
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MTSYVDKQEITASSEIDDLIFSSDPLVWSYDEQEKIRKKLVLDAF
                     RNHYKHCREYRHYCQAHKVDDNITEIDDIPVFPTSVFKFTRLLTSQENEIESWFTSSGT
                     NGLKSQVARDRLSIERLLGSVSYGMKYVGSWFDHQIELVNLGPDRFNAHNIWFKYVMSL
                     VELLYPTTFTVTEERIDFVKTLNSLERIKNQGKDLCLIGSPYFIYLLCHYMKDKKISFS
                     GDKSLYIITGGGWKSYEKESLKRDDFNHLLFDTFNLSDISQIRDIFNQVELNTCFFEDE
                     MQRKHVPPWVYARALDPETLKPVPDGTPGLMSYMDASATSYPAFIVTDDVGIISREYGK
                     YPGVLVEILRRVNTRTQKGCALSLTEAFDS"
     terminator      9425..9471
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 gctagagccg tgaacgacag ggcgaacgcc agcccgccga cggcgagggt tccgaccgct
       61 gcaactcccg gtgcaacctt gtcccggtct attctcttca ctgcaccagc tccaatctgg
      121 tgtgaatgcc cctcgtctgt tcgcgcaggc ggggggctct attcgtttgt cagcatcgaa
      181 agtagccaga tcagggatgc gttgcaaccg cgtatgccca ggtcagaaga gtcgcacaag
      241 agttgcagac ccctggaaag aaaaatggcc agagggcgaa aacaccctct gaccagcgga
      301 gcgggcgacg ggaatcgaac ccgcgtagct agtttggaag aatgggtgtc tgccgaccac
      361 atatatgggc cggtcaagat aggtttttac cccctctcgg ctgcatcctc taagtggaaa
      421 gaaattgcag gtcgtagaag cgcgttgaag cctgagagtt gcacaggagt tgcaacccgg
      481 tagccttgtt cacgacgaga ggagacctag ttggcacgtc gcggatgggg atcgctgaag
      541 actcagcgca gcgggaggat ccaagcctca tacgtcaacc cgcaggacgg tgtgaggtac
      601 tacgcgctgc agacctacga caacaagatg gactcttgtc agtacgcgaa gaaccacgcc
      661 tggccgcgag cgccagcacc gccgctctgt gcggagacct gggcaccagc cccgccgccg
      721 ccaggagcat tgccgttccc gccagctagc aacaaagcga cgttgtgtct caaaatctct
      781 gatgttacat tgcacaagat aaaaatatat catcatgaac aataaaactg tctgcttaca
      841 taaacagtaa tacaaggggt gttatgagcc atattcaacg ggaaacgtct tgctcgaggc
      901 cgcgattaaa ttccaacatg gatgctgatt tatatgggta taaatgggct cgcgataatg
      961 tcgggcaatc aggtgcgaca atctatcgct tgtatgggaa gccccatgcg ccagagttgt
     1021 ttctgaaaca tggcaaaggt agcgttgcca atgatgttac agatgagatg gtcagactaa
     1081 actggctgac ggaatttatg cctcttccga ccatcaagca ttttatccgt actcctgatg
     1141 atgcatggtt actcaccact gcgatccccg ggaaaacagc attccaggta ttagaagaat
     1201 atcctgattc aggtgaaaat attgttgatg cgctggcagt gttcctgcgc cggttgcatt
     1261 cgattcctgt ttgtaattgt ccttttaaca gcgatcgcgt atttcgtctc gctcaggcgc
     1321 aatcacgaat gaataacggt ttggttgatg cgagtgattt tgatgacgag cgtaatggct
     1381 ggcctgttga acaagtctgg aaagaaatgc ataatctttt gccattctca ccggattcag
     1441 tcgtcactca tggtgatttc tcacttgata accttatttt tgacgagggg aaattaatag
     1501 gttgtattga tgttggacga gtcggaatcg cagaccgata ccaggatctt gccatcctat
     1561 ggaactgcct cggtgagttt tctccttcat tacagaaacg gctttttcaa aaatatggta
     1621 ttgataatcc tgatatgaat aaattgcagt ttcatttgat gctcgatgag tttttctaat
     1681 cagaattggt taattggttg taacactggc agagcattac gctgacttga cgggacggcg
     1741 gctttgttga ataaatcgaa cttttgctga gttgaaggat cagatcacgc atcttcccga
     1801 caacgcagac cgttccgtgg caaagcaaaa gttcaaaatc accaactggt ccacctacaa
     1861 caaagctctc atcaaccgtg gctccctcac tttctggctg gatgatgggg cgattcaggc
     1921 ctggtatgag tcagcaacac cttcttcacg aggcagacct cactagttcc actgagcgtc
     1981 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     2041 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     2101 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct
     2161 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     2221 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     2281 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     2341 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     2401 gcattgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     2461 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     2521 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     2581 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     2641 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     2701 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcaacgcgtg
     2761 cggccgctct agaggtgacc acaacgacgc gcccgctttg atcggggacg tctgcggccg
     2821 accatttacg ggtcttgttg tcgttggcgg tcatgggccg aacatactca cccggatcgg
     2881 agggccgagg acaaggtcga acgaggggca tgacccggtg cggggcttct tgcactcggc
     2941 ataggcgagt gctaagaata acgttggcac tcgcgaccgg tgagtcgtag gtcgggacgg
     3001 tgaggccagg cccgtcgtcg cagcgagtgg cagcgaggac aacttgagcc gtccgtcgcg
     3061 ggcactgcgc ccggccagcg taagtagcgg ggttgccgtc acccggtgac ccccggtttc
     3121 atccccgatc cggaggaatc acttcgcaat ggccaagaca attgcggatc ccccgggctg
     3181 caggaattcg aatggccatg ggacgtcgac ctgaggtaat tataacccgg gccctatata
     3241 tggatccaat tgcaatgatc gtcgacagga ggactctatg aaatttggaa actttttgct
     3301 tacataccaa cctccccaat tttctcaaac agaggtaatg aaacgtttgg ttaaattagg
     3361 tcgcatctct gaggagtgtg gttttgatac cgtatggtta ctggagcatc atttcacgga
     3421 gtttggtttg cttggtaacc cttatgtcgc tgctgcatat ttacttggcg cgactaaaaa
     3481 attgaatgta ggaactgccg ctattgttct tcccacagcc catccagtac gccaacttga
     3541 agatgtgaat ttattggatc aaatgtcaaa aggacgattt cggtttggta tttgccgagg
     3601 gctttacaac aaggactttc gcgtattcgg cacagatatg aataacagtc gcgccttagc
     3661 ggaatgctgg tacgggctga taaagaatgg catgacagag ggatatatgg aagctgataa
     3721 tgaacatatc aagttccata aggtaaaagt aaaccccgcg gcgtatagca gaggtggcgc
     3781 accggtttat gtggtggctg aatcagcttc gacgactgag tgggctgctc aatttggcct
     3841 accgatgata ttaagttgga ttataaatac taacgaaaag aaagcacaac ttgagcttta
     3901 taatgaagtg gctcaagaat atgggcacga tattcataat atcgaccatt gcttatcata
     3961 tataacatct gtagatcatg actcaattaa agcgaaagag atttgccgga aatttctggg
     4021 gcattggtat gattcttatg tgaatgctac gactattttt gatgattcag accaaacaag
     4081 aggttatgat ttcaataaag ggcagtggcg tgactttgta ttaaaaggac ataaagatac
     4141 taatcgccgt attgattaca gttacgaaat caatcccgtg ggaacgccgc aggaatgtat
     4201 tgacataatt caaaaagaca ttgatgctac aggaatatca aatatttgtt gtggatttga
     4261 agctaatgga acagtagacg aaattattgc ttccatgaag ctcttccagt ctgatgtcat
     4321 gccatttctt aaagaaaaac aacgttcgct attatattag ctaaggagaa agaaatgaaa
     4381 tttggattgt tcttccttaa cttcatcaat tcaacaactg ttcaagaaca aagtatagtt
     4441 cgcatgcagg aaataacgga gtatgttgat aagttgaatt ttgaacagat tttagtgtat
     4501 gaaaatcatt tttcagataa tggtgttgtc ggcgctcctc tgactgtttc tggttttctg
     4561 ctcggtttaa cagagaaaat taaaattggt tcattaaatc acatcattac aactcatcat
     4621 cctgtcgcca tagcggagga agcttgctta ttggatcagt taagtgaagg gagatttatt
     4681 ttagggttta gtgattgcga aaaaaaagat gaaatgcatt tttttaatcg cccggttgaa
     4741 tatcaacagc aactatttga agagtgttat gaaatcatta acgatgcttt aacaacaggc
     4801 tattgtaatc cagataacga tttttatagc ttccctaaaa tatctgtaaa tccccatgct
     4861 tatacgccag gcggacctcg gaaatatgta acagcaacca gtcatcatat tgttgagtgg
     4921 gcggccaaaa aaggtattcc tctcatcttt aagtgggatg attctaatga tgttagatat
     4981 gaatatgctg aaagatataa agccgttgcg gataaatatg acgttgacct atcagagata
     5041 gaccatcagt taatgatatt agttaactat aacgaagata gtaataaagc taaacaagag
     5101 acgcgtgcat ttattagtga ttatgttctt gaaatgcacc ctaatgaaaa tttcgaaaat
     5161 aaacttgaag aaataattgc agaaaacgct gtcggaaatt atacggagtg tataactgcg
     5221 gctaagttgg caattgaaaa gtgtggtgcg aaaagtgtat tgctgtcctt tgaaccaatg
     5281 aatgatttga tgagccaaaa aaatgtaatc aatattgttg atgataatat taagaagtac
     5341 cacatgaaat atacctaata ggtaccgatc gccactagcg ccgcggtcgg aatcagcgca
     5401 ctggccgccg gagcggtcag ctacctgttg ctgcgccgct tcgctttcta gcaccgacgt
     5461 cccggcgcca gcagctacct ctcgagcaaa cgttttgcgc gcccgaaaaa taagtcgttg
     5521 cagaactttc atgaattagg ccttgctgcg cccaggctcc agtagtagaa atggagtcac
     5581 ggcagcccgg tgaagccaag gtcgaaccgg aagagaaggt tcgtcctccc gacccgggca
     5641 cccagcacgg cccccggaac ccacgcggag tcatagccgc gataatggca gaagtgttgc
     5701 gggcctgcgt aattgcgaaa ttcagatggt gccgacggcc ctttgggtgg ggctgcagcc
     5761 agaagggtcg caaaagcgcc gaggccaacc cacgcagccc acaaatgcac gcttggtaac
     5821 cgaggtacca ggaggatggc aaatatgact aaaaaaattt cattcattat taacggccag
     5881 gttgaaatct ttcccgaaag tgatgattta gtgcaatcca ttaattttgg tgataatagt
     5941 gtttacctgc caatattgaa tgactctcat gtaaaaaaca ttattgattg taatggaaat
     6001 aacgaattac ggttgcataa cattgtcaat tttctctata cggtagggca aagatggaaa
     6061 aatgaagaat actcaagacg caggacatac attcgtgact taaaaaaata tatgggatat
     6121 tcagaagaaa tggctaagct agaggccaat tggatatcta tgattttatg ttctaaaggc
     6181 ggcctttatg atgttgtaga aaatgaactt ggttctcgcc atatcatgga tgaatggcta
     6241 cctcaggatg aaagttatgt tcgggctttt ccgaaaggta aatctgtaca tctgttggca
     6301 ggtaatgttc cattatctgg gatcatgtct atattacgcg caattttaac taagaatcag
     6361 tgtattataa aaacatcgtc aaccgatcct tttaccgcta atgcattagc gttaagtttt
     6421 attgatgtag accctaatca tccgataacg cgctctttat ctgttatata ttggccccac
     6481 caaggtgata catcactcgc aaaagaaatt atgcgacatg cggatgttat tgtcgcttgg
     6541 ggagggccag atgcgattaa ttgggcggta gagcatgcgc catcttatgc tgatgtgatt
     6601 aaatttggtt ctaaaaagag tctttgcatt atcgataatc ctgttgattt gacgtccgca
     6661 gcgacaggtg cggctcatga tgtttgtttt tacgatcagc gagcttgttt ttctgcccaa
     6721 aacatatatt acatgggaaa tcattatgag gaatttaagt tagcgttgat agaaaaactt
     6781 aatctatatg cgcatatatt accgaatgcc aaaaaagatt ttgatgaaaa ggcggcctat
     6841 tctttagttc aaaaagaaag cttgtttgct ggattaaaag tagaggtgga tattcatcaa
     6901 cgttggatga ttattgagtc aaatgcaggt gtggaattta atcaaccact tggcagatgt
     6961 gtgtaccttc atcacgtcga taatattgag caaatattgc cttatgttca aaaaaataag
     7021 acgcaaacca tatctatttt tccttgggag tcatcattta aatatcgaga tgcgttagca
     7081 ttaaaaggtg cggaaaggat tgtagaagca ggaatgaata acatatttcg agttggtgga
     7141 tctcatgacg gaatgagacc gttgcaacga ttagtgacat atatttctca tgaaaggcca
     7201 tctaactata cggctaagga tgttgcggtt gaaatagaac agactcgatt cctggaagaa
     7261 gataagttcc ttgtatttgt cccataatag gtaaaagtat ggaaaatgaa tcaaaatata
     7321 aaaccatcga ccacgttatt tgtgttgaag gaaataaaaa aattcatgtt tgggaaacgc
     7381 tgccagaaga aaacagccca aagagaaaga atgccattat tattgcgtct ggttttgccc
     7441 gcaggatgga tcattttgct ggtctggcgg aatatttatc gcggaatgga tttcatgtga
     7501 tccgctatga ttcgcttcac cacgttggat tgagttcagg gacaattgat gaatttacaa
     7561 tgtctatagg aaagcagagc ttgttagcag tggttgattg gttaactaca cgaaaaataa
     7621 ataacttcgg tatgttggct tcaagcttat ctgcgcggat agcttatgca agcctatctg
     7681 aaatcaatgc ttcgttttta atcaccgcag tcggtgttgt taacttaaga tattctcttg
     7741 aaagagcttt agggtttgat tatctcagtc tacccattaa tgaattgccg gataatctag
     7801 attttgaagg ccataaattg ggtgctgaag tctttgcgag agattgtctt gattttggtt
     7861 gggaagattt agcttctaca attaataaca tgatgtatct tgatataccg tttattgctt
     7921 ttactgcaaa taacgataat tgggtcaagc aagatgaagt tatcacattg ttatcaaata
     7981 ttcgtagtaa tcgatgcaag atatattctt tgttaggaag ttcgcatgac ttgagtgaaa
     8041 atttagtggt cctgcgcaat ttttatcaat cggttacgaa agccgctatc gcgatggata
     8101 atgatcatct ggatattgat gttgatatta ctgaaccgtc atttgaacat ttaactattg
     8161 cgacagtcaa tgaacgccga atgagaattg agattgaaaa tcaagcaatt tctctgtctt
     8221 aaaggatcct gaggaggaaa aacaggtatg acttcatatg ttgataaaca agaaattaca
     8281 gcaagctcag aaattgatga tttgattttt tcgagcgatc cattagtgtg gtcttacgac
     8341 gagcaggaaa aaatcagaaa gaaacttgtg cttgatgcat ttcgtaatca ttataaacat
     8401 tgtcgagaat atcgtcacta ctgtcaggca cacaaagtag atgacaatat tacggaaatt
     8461 gatgacatac ctgtattccc aacatcggtt tttaagttta ctcgcttatt aacttctcag
     8521 gaaaacgaga ttgaaagttg gtttaccagt agcggcacga atggtttaaa aagtcaggtg
     8581 gcgcgtgaca gattaagtat tgagagactc ttaggctctg tgagttatgg catgaaatat
     8641 gttggtagtt ggtttgatca tcaaatagaa ttagtcaatt tgggaccaga tagatttaat
     8701 gctcataata tttggtttaa atatgttatg agtttggtgg aattgttata tcctacgaca
     8761 tttaccgtaa cagaagaacg aatagatttt gttaaaacat tgaatagtct tgaacgaata
     8821 aaaaatcaag ggaaagatct ttgtcttatt ggttcgccat actttattta tttactctgc
     8881 cattatatga aagataaaaa aatctcattt tctggagata aaagccttta tatcataacc
     8941 ggaggcggct ggaaaagtta cgaaaaagaa tctctgaaac gtgatgattt caatcatctt
     9001 ttatttgata ctttcaatct cagtgatatt agtcagatcc gagatatatt taatcaagtt
     9061 gaactcaaca cttgtttctt tgaggatgaa atgcagcgta aacatgttcc gccgtgggta
     9121 tatgcgcgag cgcttgatcc tgaaacgttg aaacctgtac ctgatggaac gccggggttg
     9181 atgagttata tggatgcgtc agcaaccagt tatccagcat ttattgttac cgatgatgtc
     9241 gggataatta gcagagaata tggtaagtat cccggcgtgc tcgttgaaat tttacgtcgc
     9301 gtcaatacga ggacgcagaa agggtgtgct ttaagcttaa ccgaagcgtt tgatagttga
     9361 tatcctgcaa tcaagcttat cgatgtcgac gtagttaact agcgtacgat cgactgccag
     9421 gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt
     9481 gtccggccat catggccgcg gtgatca
//
