LOCUS       sequence-74816-e         799 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial S. thermophilus #1 Cas9 (ST1) + tracrRNA expression, 
            cloDF13/spectinomycin.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 799)
  AUTHORS   Esvelt KM, Mali P, Braff JL, Moosburner M, Yaung SJ, Church GM
  TITLE     Orthogonal Cas9 proteins for RNA-guided gene regulation and editing.
  JOURNAL   Nat Methods. 2013 Sep 29. doi: 10.1038/nmeth.2681.
  PUBMED    24076762
REFERENCE   2  (bases 1 to 799)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2013 Sep 29. doi: 10.1038/nmeth.2681."
COMMENT     Sequence Label: DS-ST1cas
FEATURES             Location/Qualifiers
     source          1..799
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 caagcagctg gccaccaaga tccgcctgtg gcaccagcag ggcgagcgct gcctgtacac
       61 cggcaagacc atcagcatcc acgacctgat caacaacagc aaccagttcg aggtggacca
      121 catcctgccc ctgagcatca ccttcgacga cagcctggcc aacaaggtgc tggtgtacgc
      181 caccgccaac caggagaagg gccagcgcac cccctaccag gccctggaca gcatggacga
      241 cgcctggagc ttccgcgagc tgaaggcctt cgtgcgcgag agcaagaccc tgagcaacaa
      301 gaagaaggag tacctgctga ccgaggagga catcagcaag ttcgacgtgc gcaagaagtt
      361 catcgagcgc aacctggtgg acacccgcta cgccagccgc gtggtgctga acgccctgca
      421 ggagcacttc cgcgcccaca agatcgacac caaggtgagc gtggtgcgcg gccagttcac
      481 cagccagctg cgccgccact ggggcatcga gaagacccgc gacacctacc accaccacgc
      541 cgtggacgcc ctgatcattg cggcttctag ccagctgaac ctgtggaaga agcagaagaa
      601 caccctggtg agctacagcg aggaccagct gctggacatc gagaccggcg agctgatcag
      661 cgacgacgag tacaaggaga gcgtgttcaa ggccccctac cagcacttcg tggacaccct
      721 gaagagcaag gagttcgagg acagcatcct gttcagctac caggtggaca gcaagttcaa
      781 ccgcaagatc agcgacgcc
//
