LOCUS       sequence-74817-e         930 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  inducible (dox) expression of hSox2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 930)
  TITLE     McKinnon Lab plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 930)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLP-TRE2-hSox2
FEATURES             Location/Qualifiers
     source          1..930
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    225..258
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(353..372)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 gggtccccaa actcaccctg aagttctcag ctctagagat atcgtcgaca agcttatcga
       61 tgcggccgcg ctagcacgcg tcagctgact aggatccacg tcaggtggca cttttcgggg
      121 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
      181 catgagacaa taaccctgat aaatgcttca ataatattgg atctataact tcgtataatg
      241 tatgctatac gaagttatgg atctttcagg agctaaggaa gctaaaatgg agaaaaaaat
      301 cactggatat accaccgttg atatatccca atggcatcgt aaagaacatt ttgaggcatt
      361 tcagtcagtt gctcaatgta cctataacca gaccgttcag ctggatatta cggccttttt
      421 aaagaccgta aagaaaaata agcacaagtt ttatccggcc tttattcaca ttcttgcccg
      481 cctgatgaat gctcatccgg aatttcgtat ggcaatgaaa gacggtgagc tggtgatatg
      541 ggatagtgtt cacccttgtt acaccgtttt ccatgagcaa actgaaacgt tttcatcgct
      601 ctggagtgaa taccacgacg atttccggca gtttctacac atatattcgc aagatgtggc
      661 gtgttacggt gaaaacctgg cctatttccc taaagggttt attgagaata tgtttttcgt
      721 ctcagccaat ccctgggtga gtttcaccag ttttgattta aacgtggcca atatggacaa
      781 cttcttcgcc cccgttttca ccatgggcaa atattatacg caaggcgaca aggtgctgat
      841 gccgctggcg attcaggttc atcatgccgt ttgtgatggc ttccatgtcg gcagaatgct
      901 taatgaatta caacagtact gcgatgagtg
//
